栽培模式对沙棘人工林土壤微生物群落结构和参与氮循环功能基因的影响

应用生态学报

栽培模式对沙棘人工林土壤微生物群落结构和参与氮循环功能基因的影响

杨丹^1,2,余旋^1,2**,刘旭³,刘金良^1,2,张顺祥^1,2,于泽群^1,2

(¹西北农林科技大学林学系, 陕西杨凌 712100; ^2西北农林科技大学教育部西部环境与生态重点实验室, 陕西杨凌 712100;
^3西北农林科技大学葡萄酒学院, 陕西杨凌 712100)

出版日期:2015-12-18 发布日期:2015-12-18

Effect of afforestation modes on soil microbial community and nitrogen functional genes in Hippophae rhamnoides plantation.

YANG Dan^1,2, YU Xuan^1,2, LIU Xu³, LIU Jin-liang^1,2, ZHANG Shun-xiang^1,2, YU Ze-qun^1,2

(¹Department of Forestry, Northwest A&F University, Yangling 712100, Shaanxi, China; ^2Ministry of Education Key Laboratory of Environment and Ecology in West China, Yangling 712100, Shaanxi, China; ³College of Enology, Northwest A&F University, Yangling 712100, Shaanxi, China)

Online:2015-12-18 Published:2015-12-18

摘要/Abstract

摘要： 以沙棘×油松混交林、沙棘×侧柏混交林、沙棘×刺槐混交林、沙棘纯林的土壤为研究对象,对其土壤微生物种群结构、参与氮循环功能基因丰度进行了检测.结果表明: 沙棘与油松或侧柏混交能显著增加林地土壤总磷脂脂肪酸(总PLFA)、细菌脂肪酸(BPLFA)和革兰氏阳性菌脂肪酸(G⁺PLFA)含量,而真菌脂肪酸 (FPLFA)含量无明显变化.两种混交林地土壤的nifH、amoA、nirK和narG基因丰度明显高于沙棘纯林土壤.土壤总PLFA、G⁺PLFA、革兰氏阴性菌生物量(G^-PLFA)和4种功能基因的丰度均与土壤pH、土壤有机碳、总氮、NH₄⁺-N、速效钾呈显著正相关.不同栽培模式人工林土壤微生物群落和基因丰度的差异主要与土壤理化特性有关.沙棘与油松或侧柏混交为当地2种较好的栽培模式,能有效地改善土壤质量.

Abstract:

The study aimed to assess the effect of different afforestation modes on microbial composition and nitrogen functional genes in soil. Soil samples from a pure Hippophae rhamnoides stand (SS) and three mixed stands, namely, H. rhamnoides and Pinus tabuliformis (SY), H. rhamnoides and Platycladus orientalis (SB), H. rhamnoides and Robinia pseucdoacacia (SC) were selected. The results showed that the total PLFA (TPLFA), bacterial PLFA, gram positive bacterial PLFA (G⁺PLFA) were significantly higher in soil samples from other three stands than those of the pure one. However, no significant difference was found for fungal PLFA among them. The abundance of nifH, amoA, nirK and narG genes were higher in SY and SC than in SS. The TPLFA, G⁺PLFA, gram negative bacterial PLFA (G^-PLFA), and all of the detected gene abundance were significantly and positively correlated with soil pH, total organic carbon, total nitrogen, ammonium nitrogen and available potassium. Afforestation modes affected indirectly soil microbial composition and functional genes through soil properties. Mixing P. tabuliformis or P. orientalis with H. rhamnoides might be suitable afforestation modes, which might improve soil quality.

杨丹1,2,余旋1,2**,刘旭3,刘金良1,2,张顺祥1,2,于泽群1,2. 栽培模式对沙棘人工林土壤微生物群落结构和参与氮循环功能基因的影响[J]. 应用生态学报.

YANG Dan1,2, YU Xuan1,2, LIU Xu3, LIU Jin-liang1,2, ZHANG Shun-xiang1,2, YU Ze-qun1,2. Effect of afforestation modes on soil microbial community and nitrogen functional genes in Hippophae rhamnoides plantation.[J]. Chinese Journal of Applied Ecology.