Abstract: The carbon flux of subtropical bamboo forest ecosystem was continuously measured using eddy covariance technique in Anji County of Zhejiang Province, China. The monthly net ecosystem productivity (NEP), ecosystem respiration (R_e) and gross ecosystem productivity (GEP) data from 2011 to 2013 were selected to analyze the impacts of seasonal high temperature and drought on the carbon flux of bamboo forest ecosystem. The results showed that there were big differences among annual NEP of bamboo forest from 2011 to 2013. Because of the asynchronization of precipitation and heat, the seasonal high temperature and drought in July and August of 2013 caused significant decline in NEP by 59.9% and 80.0% when compared with the same months in 2011. Correlation analysis of the NEP, R_e, GEP and environmental factors suggested that the atmosphere temperatures were significantly correlated with R_e and GEP in 2011 and 2013 （P<0.05）. However, to air and soil moisture, R_e and GEP had different responses, that was, GEP was more vulnerable by the decrease of the soil moisture compared with R_e. Besides, the raising of saturation vapour pressure promoted the R_e modestly but inhibited the GEP, which was supposed to be the main reason for NEP decrease of bamboo forest ecosystem in Anji from July to August in 2013.