Abstract: Nitrogen limitation is common in terrestrial ecosystems, and it is particularly severe in damaged ecosystems in arid regions. Biological soil crusts (BSCs), as a crucial component of recovered vegetation, play a vital role in nitrogen fixation during the ecological restoration processes of damaged ecosystems in arid and semiarid regions. In this study, two dominant types of BSCs (i.e., cyanobacterialalgal crusts and moss crusts) that are widely distributed in the revegetated area of Heidaigou open pit coal mine were investigated. Samples were collected in the field and their nitrogenase activities (NA) were measured in the laboratory. The responses of NA to different hydrothermal factors and the relationships between NA and herbs in addition to crust coverage were analyzed. The results indicated that BSCs under reconstructed vegetation at different succession stages, abandoned land and natural vegetation showed values of NA ranging from 9 to 150 μmol C₂H₄·m^-2·h^-1, and the NA value of algae crust (77 μmol C₂H₄·m^-2·h^-1) was markedly higher than that of moss crust (17 μmol C₂H₄·m^-2·h^-1). In the revegetated area, cyanobacterialalgal crust and moss crust under shrubherb had higher NA values than those of crusts under arborshrub and arborshrubherb. The relationship between NA of the two BSCs and soil relative water content (10%-100%) as well as culture temperature (5-45 ℃) were of quadratic function. With elevated water content and cultural temperature, the NA values increased at the initial stage and then decreased, and reached the maximum value at 25 ℃ of cultural temperature and 60% or 80% of relative water content. The NA of cyanobacterialalgal crust had a significant quadratic function with herb coverage, as NA declined when herb coverage was higher than 20%. A significant negative correlation was observed between the NA of moss crusts and herb coverage. The NA values of the two types of BSCs had a significant positive correlation with crust coverage, since the NA was enhanced when the crust coverage was increased. We concluded that the different NA of the two BSCs in the re-vegetated area of Heidaigou open pit coal mine were caused by the composition of cryptograms. In addition, the differences of hydrothermal conditions and the composition of herb or crust coverage at different succession stages were also the contribution factors. Therefore, BSC construction and nitrogen fixation in re-vegetated areas is an important symbol for sustainable development in ecosystems.