接种刺状无梗囊霉对番茄生长及psy1与psy2基因表达的影响

应用生态学报

接种刺状无梗囊霉对番茄生长及psy1与psy2基因表达的影响

李可¹,许建军²,王路遥¹,王楚南¹,于琛¹,郭坚华^1*

(¹南京农业大学植物保护学院/农作物生物灾害综合治理教育部重点实验室/江苏省生物源农药工程中心, 南京 210095;
²新疆农业科学院植物保护研究所, 乌鲁木齐 830091)

出版日期:2016-02-18 发布日期:2016-02-18

Effects of Acaulospora spinosa on plant growth and lycopene related genes (psy1 and psy2) expression of tomato.

LI Ke¹, XU Jian-jun², WANG Lu-yao¹, WANG Chu-nan¹,YU Chen¹, GUO Jian-hua^1*

(¹College of Plant Protection, Nanjing Agricultural University/Key Laboratory of Integrated Management of Crop Diseases and Pest, Ministry of Education/Jiangsu Province Engineering Center of Bioresource Pesticide, Nanjing 210095, China; ²Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China).

Online:2016-02-18 Published:2016-02-18

摘要/Abstract

摘要： 本研究利用前期筛选获得的丛枝菌根真菌(AMF)刺状无梗囊霉XJ27侵染矮化观赏型番茄品种“小汤姆”，分析番茄植株生物量、叶绿素含量以及果实番茄红素含量等相关指标，并采用半定量RT-PCR与实时PCR检测对番茄红素合成相关基因psy1与psy2的表达情况.结果表明：与对照组相比，刺状无梗囊霉处理组番茄的生物量显著增加，增产效果明显；番茄红素合成相关基因psy1与psy2表达增强，果实中的番茄红素含量显著提高.表明刺状无梗囊霉XJ27是一株很有应用潜力的AM真菌.

Abstract: In the present study, an arbuscular mycorrhizal fungus (AMF) strain Acaulospora spinosa XJ27, previously isolated from tomato root rhizosphere in Xinjiang’s suburbs, was used to inoculate root systems of tomato cultivar “MicroTom”. The biomass, contents of chlorophyll in leaves and lycopene in fruits of plants between the treatments with and without XJ27 were analyzed, and the expression level of lycopene biosynthesis relevant genes (psy1 and psy2) investigated by semi quantitativePCR and realtime PCR. The results indicated that the application of XJ27 to the rhizosphere of tomato increased the biomass and yield of the treated plants, the expression of psy1 and psy2 was improved, and the amount of lycopene accumulation in tomato. It suggested Acaulospora spinosa XJ27 has a great potential in utilization.

李可1,许建军2,王路遥1,王楚南1,于琛1,郭坚华1*. 接种刺状无梗囊霉对番茄生长及psy1与psy2基因表达的影响[J]. 应用生态学报.

LI Ke1, XU Jian-jun2, WANG Lu-yao1, WANG Chu-nan1,YU Chen1, GUO Jian-hua1*. Effects of Acaulospora spinosa on plant growth and lycopene related genes (psy1 and psy2) expression of tomato.[J]. Chinese Journal of Applied Ecology.