欢迎访问《应用生态学报》官方网站,今天是 分享到:

应用生态学报 ›› 2017, Vol. 28 ›› Issue (10): 3274-3280.doi: 10.13287/j.1001-9332.201710.004

• 目录 • 上一篇    下一篇

外源水杨酸对干旱胁迫下小冠花种子萌发及幼芽生理特性的影响

马乐元,陈年来,韩国君,李良   

  1. 甘肃农业大学资源与环境学院, 兰州 730070
  • 收稿日期:2017-03-27 修回日期:2017-07-12 出版日期:2017-10-18 发布日期:2017-10-18
  • 作者简介:马乐元,男,1984年生,博士研究生.主要从事植物逆境生理生态研究.E-mail:119256800@qq.com
  • 基金资助:

    本文由国家自然基金青年科学基金项目(31200320)和甘肃农业大学盛彤笙科技创新基金项目(GSAU-STS-1332)资助

Effects of exogenous salicylic acid on seed germination and physiological characteristics of Coronilla varia under drought stress.

MA Le-yuan, CHEN Nian-lai*, HAN Guo-jun, LI Liang   

  1. College of Resources and Environmental Sciences, Gansu Agricultural University, Lanzhou 730070, China
  • Received:2017-03-27 Revised:2017-07-12 Online:2017-10-18 Published:2017-10-18
  • Supported by:

    This work was supported by the National Natural Foundation for Young Scientists of China (31200320) and the Science and Technology Innovation Foundation of Sheng Tong-sheng in Gansu Agricultural University (GSAU-STS-1332).

摘要: 以豆科牧草“绿宝石”小冠花为试材,研究PEG-6000(浓度8%和12%)模拟干旱胁迫下不同浓度外源水杨酸(0、0.5、1.0和2.0 mmol·L-1)对小冠花种子萌发和幼芽生理特性的影响.结果表明: 0.5~1.0 mmol·L-1水杨酸显著提高了干旱胁迫下小冠花种子发芽率、发芽势、发芽指数、活力指数和芽长,12%PEG胁迫下1.0 mmol·L-1水杨酸处理小冠花幼芽干质量显著高于干旱处理.0.5~1.0 mmol·L-1水杨酸处理显著提高了干旱胁迫下小冠花幼芽脯氨酸、可溶性蛋白含量,显著提高了过氧化氢酶、过氧化物酶、超氧化物歧化酶活性,显著降低了幼芽细胞电解质渗透率、H2O2含量、O2产生速率,其中以1.0 mmol·L-1水杨酸处理效果最好.水杨酸浓度超过2.0 mmol·L-1时对干旱胁迫没有缓解效应.表明适宜浓度的水杨酸(0.5~1.0 mmol·L-1)可以提高小冠花幼芽渗透调节能力和抗氧化能力,促进小冠花生长,缓解干旱胁迫伤害.

Abstract: This research investigated the effects of different concentrations (0, 0.5, 1.0, 2.0 mmol·L-1) of salicylic acid on the seed germination and physiological characteristics of legume forage Coronilla varia (cultivar ‘Lvbaoshi’) under PEG-6000 (concentration 8% and 12%) simulated drought stress. The results showed that under drought stress, 0.5-1.0 mmol·L-1salicylic acid significantly increased germination percentage, germination vigour, germination index, vitality index and bud length of C. varia. Under the stress of 12% PEG, the dry mass of C. varia seedlings processed by 1.0 mmol·L-1 salicylic acid was significantly higher than that under drought stress. 0.5-1.0 mmol·L-1 salicylic acid processing significantly increased proline, soluble protein content, the activities of catalase, peroxidase and superoxide dismutase of C. varia seedlings under drought stress, but cell electrolyte permeability, H2O2 content and O2 production rate of seedlings were significantly decreased. 1.0 mmol·L-1 salicylic acid produced the best results. When the concentration of salicylic acid was beyond 2.0 mmol·L-1, no mitigation effect was observed on the seed germination and growth of seedlings under drought stress. It was concluded that salicylic acid at appropriate concentrations could effectively improve osmotic regulation, antioxidation and mitigate the damage of drought stress so as to promote the growth of C. varia seedlings.