Abstract: Based on the small scale characteristics of the basin, which is a kind of special landform influenced by the human activities obviously in Karst mountain area, the system grid method and the semivariogram model were used to analyze the 15 basins which belonged to three types in the territory of Guizhou Province to reveal the general pattern of spatial evolution of land use intensity and the mutual relationship with the landscape pattern change of the typical basins in the Karst mountainous area of Guizhou Province in 2005, 2010 and 2015. The results showed that the dynamic spatial evolution of land use intensity and response mode of landscape pattern showed obvious difference under the different basin types. In general, with the decrease of economic level of the basins from high to low, the land use intensity decreased in the form of a gradient. At the level of microcosmic evolution, the land use intensity of basins in the center of the cities and counties descended first and then ascended in the form of concentric band around the periphery of the center in the basin, and the spatial evolution trend of this kind of basin exhibited a stable and expanded form. The land use intensity of basins surrounding cities and towns, with a high intensity center, that could either increase or decrease, exhibited a fluctuating spatial evolution trend. The land use intensity of remote basins was basically stable, even slightly weakened and its space evolution was stable and debilitating. Because of the divergence of dominant function of the basins, there were five different modes of response between the change of land use intensity and landscape pattern of basins. During the study period, the spatial evolution of land use intensity of these 3 basin types was driven by natural and human factors. The interaction among various factors formed the typical characteristics of the land use intensity spatial evolution type of different basins.