生物有机肥对猕猴桃土壤酶活性和微生物群落的影响

doi:10.13287/j.1001-9332.201603.012

应用生态学报 ›› 2016, Vol. 27 ›› Issue (3): 829-837.doi: 10.13287/j.1001-9332.201603.012

生物有机肥对猕猴桃土壤酶活性和微生物群落的影响

孙家骏¹, 付青霞², 谷洁^1,3*, 王小娟¹, 高华^1,3

¹西北农林科技大学资源环境学院, 陕西杨凌 712100;
²江苏省南通滨海园区管委会, 江苏南通 226000;
³陕西省循环农业工程技术研究中心, 陕西杨凌 712100

收稿日期:2015-07-08 出版日期:2016-03-18 发布日期:2016-03-18
通讯作者: * E-mail: gujoyer@sina.com
作者简介:孙家骏,女,1990年生,硕士研究生.主要从事环境微生物研究.E-mail:sunjiajun09@126.com
基金资助:
本文由国家自然科学基金项目(41171203)、公益性行业(农业)科研专项(201303094)和杨凌示范区科技计划项目(2015NY-25)资助

Effects of bio-organic fertilizer on soil enzyme activities and microbial community in kiwifruit orchard

SUN Jia-jun¹, FU Qing-xia², GU Jie^1,3*, WANG Xiao-juan¹, GAO Hua^1,3

¹Collage of Resource and Environment, Northwest A＆F University, Yangling 712100, Shaanxi, China;
²Nantong Binhai Park Administrative Committee, Nantong 226000, Jiangsu, China;
³Shaanxi Province Research Center of Recycle Agricultural Engineering and Technology, Yangling 712100, Shaanxi, China

Received:2015-07-08 Online:2016-03-18 Published:2016-03-18
Contact: * E-mail: gujoyer@sina.com
Supported by:
This work was supported by the National Natural Science Foundation of China (41171203), the Special Fund for Agro-scientific Research in the Public Interest (201303094) and the Scientific Planning Project of Yangling Demonstration Zone, China (2015NY-25).

摘要/Abstract

摘要： 采用田间试验,研究了不施肥、施用生物有机肥、传统有机肥和化肥对猕猴桃土壤酶活性、微生物群落结构及其代谢的影响.结果表明: 施用生物有机肥在猕猴桃生育期内可显著提高土壤蔗糖酶和荧光素二乙酸酯水解酶活性,分别比对照高出12.2%～129.4%、18.8%～87.4%.施用生物有机肥在膨大期和成熟期的土壤脲酶和酸性磷酸酶活性显著高于其他处理.Biolog微平板法测定结果表明,生物有机肥在猕猴桃生育期内提高了土壤微生物的平均每孔颜色变化率,增加了物种多样性、丰富度和均匀度,改变了微生物对6大类碳源的相对利用率,降低了微生物对氨基酸类碳源的相对利用能力,提高了微生物对多酚类和多胺类碳源的相对利用能力;主成分分析表明,对土壤中微生物起分异作用的碳源种类主要是糖类、氨基酸类和羧酸类.

Abstract: A field experiment was conducted to compare the effects of three fertilizer managements (bio-organic fertilizer, traditional organic fertilizer and chemical fertilizer) and a no-fertilizer control on soil enzyme activities and microbial community functional diversity in a kiwifruit orchard. The results showed that the soil invertase and FDA hydrolase activities in the bio-organic fertilizer treatment were 12.2%-129.4% and 18.8%-87.4% higher than those in the no-fertilizer control during kiwifruit growth period, respectively. The application of bio-organic fertilizer also increased soil urease and acid phosphatase activities at the expanding stage and maturity stage. The Biolog results suggested that bio-organic fertilizer treatment improved the average well color development (AWCD) and increased the species diversity, richness and evenness. The relative ratios of six groups of carbon sources by microbes were changed to some extent after the application of bio-organic fertilizer. Compared with the no-fertilizer control, bio-organic fertilizer application decreased the capacity of microbes in using amino acids, but enhanced the utilization of polyphenols and polyamines. The principal components analysis demonstrated that the differentiation of microbial community was mainly in utilization of carbohydrates, amino acids and carboxylic acids.

孙家骏, 付青霞, 谷洁, 王小娟, 高华. 生物有机肥对猕猴桃土壤酶活性和微生物群落的影响[J]. 应用生态学报, 2016, 27(3): 829-837.

SUN Jia-jun, FU Qing-xia, GU Jie, WANG Xiao-juan, GAO Hua. Effects of bio-organic fertilizer on soil enzyme activities and microbial community in kiwifruit orchard[J]. Chinese Journal of Applied Ecology, 2016, 27(3): 829-837.

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生物有机肥对猕猴桃土壤酶活性和微生物群落的影响

Effects of bio-organic fertilizer on soil enzyme activities and microbial community in kiwifruit orchard

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