川滇柳与青杨属间嫁接幼苗对氮素缺乏的生理响应

doi:10.13287/j.1001-9332.201712.004

应用生态学报 ›› 2017, Vol. 28 ›› Issue (12): 3833-3840.doi: 10.13287/j.1001-9332.201712.004

川滇柳与青杨属间嫁接幼苗对氮素缺乏的生理响应

韩清泉^{1, 2}, 宋海凤^{1, 2}, 唐铎腾^{1, 2}, 张胜^1*

¹中国科学院、水利部成都山地灾害与环境研究所, 山地表生过程与生态调控重点实验室, 成都 610041
²中国科学院大学, 北京 100049

收稿日期:2017-05-10 出版日期:2017-12-18 发布日期:2017-12-18
通讯作者: * E-mail: zhangsheng@imde.ac.cn
作者简介:韩清泉,女,1987年生,博士研究生.主要从事树木逆境生理及分子生物学研究.E-mail:hanqq@imde.ac.cn
基金资助:
本文由国家自然科学基金优秀青年基金项目(31322014)和中国科学院前沿重点研究项目(QYZDB-SSW-DQC037)资助

Physiological responses of Salix rehderiana and Populus cathayana grafted seedlings to nitrogen deficiency

HAN Qing-quan^1,2, SONG Hai-feng^1,2, TANG Duo-teng^1,2, ZHANG Sheng^1*

¹Key Laboratory of Mountain Surface Processes and Ecological Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences and Ministry of Water Sources, Chengdu 610041, China
²University of Chinese Academy of Sciences, Beijing 100049, China

Received:2017-05-10 Online:2017-12-18 Published:2017-12-18
Contact: * E-mail: zhangsheng@imde.ac.cn
Supported by:
This work was supported by the Excellent Young Scientist Program of the National Natural Science Foundation of China (31322014) and the Frontier Science Key Research Program of Chinese Academy of Sciences (QYZDB-SSW-DQC037)

摘要/Abstract

摘要： 同为杨柳科的柳属和杨属植物对氮素缺乏的形态和生理响应不同.本文通过川滇柳和青杨远缘嫁接,研究不同嫁接组合幼苗在氮缺乏条件下的成活率、生长状况、生物量累积与分配、气体交换参数及非结构性碳水化合物的差异响应,分析杨柳科植物远缘嫁接的可行性及嫁接对植物抗缺氮能力的影响.结果表明:川滇柳和青杨远缘嫁接的亲和性较好,川滇柳/青杨(S/P)和青杨/川滇柳(P/S)嫁接组合的成活率分别为74%和96%;缺氮显著降低了所有嫁接组合幼苗的成活率,其中S/P幼苗的成活率降低最明显,仅为53.3%,而P/S幼苗的成活率为86.7%.无论对照还是缺氮条件下,青杨做接穗的嫁接组合(P/P和P/S)的株高、基茎、生物量积累、净光合速率均大于川滇柳做接穗的嫁接组合(S/S和S/P);缺氮显著减少了所有嫁接组合的生长、生物量及净光合速率.川滇柳做砧木的嫁接组合(S/S和P/S)的根冠比显著高于青杨做砧木的嫁接组合(S/P和P/P),表明川滇柳可将更多的光合产物分配到地下部分,而青杨则将更多的光合产物投入到地上部分.各嫁接组合根中非结构性碳水化合物受缺氮胁迫最明显,除S/P嫁接组合外,P/P、S/S和P/S根中的淀粉、果糖、蔗糖和可溶性总糖浓度均显著升高,且表现为P/P和P/S显著高于S/S和S/P.

关键词: 缺氮, 光合作用, 非结构性碳水化合物, 嫁接

Abstract: Morphological and physiological responses of Salix plants are different from Populus to nitrogen (N) deficiency. In this study, grafting technology was used in S. rehderiana and P. cathayana to investigate the graft compatibility of Salix and Populus, and whether grafting could improve the resistance to N deficiency in Salicaceae plants. The survival rate, growth, biomass accumulation and allocation, gas exchange parameters and non-structural carbohydrates (NSCs) were measured to evaluate the resistance to N deficiency among different grafting combinations. The results showed that the graft compatibility between S. rehderiana and P. cathayana was quite high. The survival rate was 74% and 96% in S/P (S. rehderiana was used as scions and P. cathayana as rootstocks) and P/S (P. cathayana was used as scions and S. rehderiana as rootstocks) combinations, respectively. N deficiency reduced the survival rate in all grafting combinations, which were 53.3% and 86.7% in S/P and P/S, respectively. The survival rate of S/P was lower than that of the other grafting combinations. Under control and N-deficient conditions, the height, basal diameter, biomass and net photosynthetic rate (P_n) of P/P and P/S combinations were higher than those of S/S and S/P combinations. N deficiency significantly reduced growth rate, biomass accumulation and P_n in all grafting combinations. The rate between root biomass and aboveground biomass of S. rehderiana rootstock combinations (S/S and P/S) was significantly higher than those of P. catha-yana rootstock combinations (P/P and S/P) under both control and N-deficient conditions. It indicated that more photosynthates might be allocated to belowground in S. rehderiana, while to aboveground in P. cathayana. The NSCs in roots of all grafting combinations were more sensitive to N deficiency than in stems and leaves. Except for the S/P combination, the starch, fructose, sucrose and total soluble sugar concentrations were significantly increased in roots in P/P, S/S and P/S combinations. Additionally, under N-deficient condition, the NSCs contents were significantly higher in P/P and P/S combinations than in S/S and S/P combinations.

Key words: non-structural carbohydrate, graft, nitrogen deficiency, photosynthesis

韩清泉, 宋海凤, 唐铎腾, 张胜. 川滇柳与青杨属间嫁接幼苗对氮素缺乏的生理响应[J]. 应用生态学报, 2017, 28(12): 3833-3840.

HAN Qing-quan, SONG Hai-feng, TANG Duo-teng, ZHANG Sheng. Physiological responses of Salix rehderiana and Populus cathayana grafted seedlings to nitrogen deficiency[J]. Chinese Journal of Applied Ecology, 2017, 28(12): 3833-3840.

参考文献

[1] Guo W (郭炜), Hu N (胡南), Li X-P (李小平), et al. Comparative analysis of the performance of different lysis buffers for measuring the ploidy level of Salicaceae species. Bulletin of Botanical Research (植物研究), 2016, 36(5): 782-789 (in Chinese)
[2] Ding T-Y (丁托娅). Origin, divergence and geographi-cal, distribution of Salicaceae. Acta Botanica Yunnanica (云南植物研究), 1995, 17(3): 277-290 (in Chinese)
[3] Tang G-M (唐桂梅), Jiang W-B (姜卫兵), Weng M-L (翁忙玲). Willow family and its application in landscaping. Chinese Agricultural Science Bulletin (中国农学通报), 2007, 23(3): 318-323 (in Chinese)
[4] Zamora DS, Apostol KG, Wyatt GJ. Biomass production and potential ethanol yields of shrub willow hybrids and native willow accessions after a single 3-year harvest cycle on marginal lands in central Minnesota, USA. Agroforestry Systems, 2014, 88: 593-606
[5] Körner C. Alpine Plant Life-Functional Plant Ecology of High Mountain Ecosystems. Berlin: Springer-Verlag, 2003
[6] Marschner P. Marschners’s Mineral Nutrition of Higher Plants. 3nd Ed. Amsterdam: Elsevier, 2012
[7] Boyce RL, Larson JR, Sanford RL. Phosphorus and nitrogen limitations to photosynthesis in Rocky Mountain bristlecone pine (Pinas aristata) in Colorado. Tree Physiology, 2006, 26: 1477-1486
[8] Zhang S, Jiang H, Zhao HX, et al. Sexually different physiological responses of Populus cathayana to nitrogen and phosphorus deficiencies. Tree Physiology, 2014, 34: 343-354
[9] Chen J, Dong TF, Duan BL, et al. Sexual competition and N supply interactively affect the dimorphism and competiveness of opposite sexes in Populus cathayana. Plant, Cell & Environment, 2015, 38: 1285-1298
[10] Song MY, Yu L, Jiang YL, et al. Nitrogen-controlled intra- and interspecific competition between Populus purdomii and Salix rehderiana drive primary succession in the Gongga Mountain glacier retreat area. Tree Physiology, 2017, 3: 1-16
[11] Pina A, Errea P. A review of new advances in mechanism of graft compatibility-incompatibility. Scientia Horticulturae, 2005, 106: 1-11
[12] García-Sánchez F, Syvertsen JP, Gimeno V, et al. Responses to flooding and drought stress by two citrus rootstock seedlings with different water-use efficiency. Phy-siologia Plantarum, 2007, 130: 532-542
[13] Han Y, Wang YH, Jiang H, et al. Reciprocal grafting separates the roles of the root and shoot in sex-related drought responses in Populus cathayana males and females. Plant, Cell & Environment, 2013, 36: 356-364
[14] Poor RE. Investigating the effect of grafted watermelon on tolerance to drought and salinity. Journal of Novel Applied Sciences, 2015, 4: 670-673
[15] Stegemann S, Bock R. Exchange of genetic material between cells in plant tissue grafts. Science, 2009, 324: 649-651
[16] Gurdon G, Svab Z, Feng YP, et al. Cell-to-cell movement of mitochondria in plants. Proceedings of the National Academy of Sciences of the United States of America, 2016, 113: 3395-3400
[17] Lewsey MG, Hardcastle TJ, Melnyk CW, et al. Mobile small RNAs regulate genome-wide DNA methylation. Proceedings of the National Academy of Sciences of the United States of America, 2016, 113: E801-E810
[18] Fuentes I, Stegemann S, Golczyk H, et al. Horizontal genome transfer as an asexual path to the formation of new species. Nature, 2014, 511: 232-235
[19] Li B-Y (李保印), Liu Y-S (刘用生), Zhou X-M (周秀梅), et al. Study on distant grafting of Salix and Popu-lus. Bulletin of Biology (生物学通报), 2004, 39(10): 19-20 (in Chinese)
[20] Zhang S, Zhou R, Zhao HX, et al. iTRAQ-based quantitative proteomic analysis gives insight into sexually different metabolic processes of poplars under nitrogen and phosphorus deficiencies. Proteomics, 2016, 16: 614-628
[21] Yemm EW, Willis AJ. The estimation of carbohydrates in plant extracts by anthrone. Biochemical Journal, 1954, 57: 508-514
[22] Murata T, Akazawa T, Fukuchi S. Enzymic mechanism of starch of starch breakdown in germinating rice seeds. I. An analytical study. Plant Physiology, 1968, 43: 1899-1905
[23] Dubois M, Gilles KA, Hamilton JK, et al. Colorimetric method of determination of sugars and related substances. Analytical Chemistry, 1956, 28: 350-356
[24] Liu Y-S (刘用生). The theoretical and practical significance of plant distant grafting in ancient and modern China. Studies in the History of Natural Sciences (自然科学史研究), 2001, 20(4): 352-361 (in Chinese)
[25] Shao G-Y (邵果园), Lu F-F (陆方方). In vitro distant grafting with an ISSR analysis. Journal of Zhejiang Forestry College (浙江农林大学学报), 2010, 27(4): 630-634 (in Chinese)
[26] Lu Y-T (卢吟涛), Shao G-Y (邵果园), Lu F-F (陆方方). Experiment on in-vitro distant grafting of seven plant species. Journal of Zhejiang Forestry Science and Technology (浙江林业科技), 2013, 33(6): 57-60 (in Chinese)
[27] Mudge K, Janick J, Scofield S, et al. A history of graf-ting. Horticultural Reviews, 2009, 35: 437-493
[28] Zhao Y (赵燕), Dong W-Y (董雯怡), Zhang Z-Y (张志毅), et al. Effects of fertilization on seedling growth and photosynthesis of hybrid clone seedlings of Populus tomentosa. Scientia Silvae Sinicae (林业科学), 2010, 46(4): 70-77 (in Chinese)
[29] McDowell N, Pockman WT, Allen CD, et al. Mechanisms of plant survival and mortality during drought: Why do some plants survive while others succumb to drought? New Phytologist, 2008, 178: 719-739
[30] Temperini O, Calabrese N, Temperini A, et al. Grafting artichoke onto cardoon rootstocks: Graft compatibility, yield and Verticillium wilt incidence. Scientia Horticulturae, 2013, 149: 22-27
[31] Dong TF, Duan BL, Zhang S, et al. Growth, biomass allocation and photosynthetic responses are related to intensity of root severance and soil moisture conditions in the plantation tree Cunninghamia lanceolata. Tree Physio-logy, 2016, 36: 807-817
[32] Kano M, Inukai Y, Kitano H, et al. Root plasticity as the key root trait for adaptation to various intensities of drought stress in rice. Plant and Soil, 2011, 342: 117-128
[33] Lu G-C (卢广超), Xu J-X (许建新), Xue L (薛立), et al. Comprehensive evaluation on photosynthetic and fluorescence characteristics in seedlings of 4 drought resistance species. Acta Ecologica Sinica (生态学报), 2013, 33(24): 7872-7881 (in Chinese)
[34] Way DA, Seegobin SD, Sage RF. The effect of carbon and nutrient loading during nursery culture on the growth of black spruce seedlings: A six-year field study. New Forests, 2007, 34: 307-312
[35] Adams HD, Germino MJ, Breshears DD, et al. Nonstructural leaf carbohydrate dynamics of Pinus edulis during drought-induced tree mortality reveal role for carbon metabolism in mortality mechanism. New Phytologist, 2013, 197: 1142-1151
[36] Guo Q, Li J, Zhang YB, et al. Species-specific competition and N fertilization regulate non-structural carbohydrate contents in two Larix species. Forest Ecology and Management, 2016, 364: 60-69
[37] Muller B, Pantin F, Génard M, et al. Water deficits uncouple growth from photosynthesis, increase C content, and modify the relationships between C and growth in sink organs. Journal of Experimental Botany, 2011, 62: 1715-1729
[38] Blum A. Osmotic adjustment is a prime drought stress adaptive engine in support of plant production. Plant, Cell & Environment, 2017, 40: 4-10
[39] Myers JA, Kitajima K. Carbohydrate storage enhances seedling shade and stress tolerance in a neotropical forest. Journal of Ecology, 2007, 95: 383-395
[40] Wiley E, Huepenbecker S, Casper BB, et al. The effects of defoliation on carbon allocation: Can carbon limitation reduce growth in favour of storage? Tree Physio-logy, 2013, 33: 1216-1228
[41] O’Brien MJ, Leuzinger S, Philipson CD, et al. Drought survival of tropical tree seedlings enhanced by non-structural carbohydrate levels. Nature Climate Change, 2014, 4: 710-714
[42] Pan Q-M (潘庆民), Han X-G (韩兴国), Bai Y-F (白永飞), et al. Advances in physiology and ecology studies on stored non-structure carbohydrates in plants. Chinese Bulletin of Botany (植物学通报), 2002, 19(1): 30-38 (in Chinese)
[43] Li N-N (李娜妮), He N-P (何念鹏), Yu G-R (于贵瑞). Evaluation of leaf non-structural carbohydrate contents in typical forest ecosystems in northeast China. Acta Ecologica Sinica (生态学报), 2016, 36(2): 430-438 (in Chinese)
[44] Aronsson P, Perttu K. Willow vegetation filters for wastewater treatment and soil remediation combined with biomass production. The Forestry Chronicle, 2001, 77: 293-299
[45] Kuzovkina YA, Knee M, Quigley MF. Cadmium and copper uptake and translocation in five willow (Salix L.) species. International Journal of Phytoremediation, 2004, 6: 269-287

川滇柳与青杨属间嫁接幼苗对氮素缺乏的生理响应

Physiological responses of Salix rehderiana and Populus cathayana grafted seedlings to nitrogen deficiency

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	王复标, 杨小龙, 康华靖, 叶子飘. 不同环境条件下光合作用对光响应模型的研究进展 [J]. 应用生态学报, 2023, 34(7): 1995-2005.
[2]	张慧玉, 岳丹斐, 潘晓娇, 郝露露, 刘伟成, 郑春芳. 5-羟色胺在红树植物秋茄幼苗抗寒中的作用 [J]. 应用生态学报, 2023, 34(5): 1263-1271.
[3]	张鸿志, 张文娥, 樊卫国, 欧阳章维, 蔡虎, 郝振坤, 王瑞璞, 潘学军. 核桃/刺梨复合种植对刺梨生长、果实产量、品质和土壤性质的影响 [J]. 应用生态学报, 2023, 34(3): 699-707.
[4]	张恒, 贾辉, 崔莹莹, 何露露, 肖好燕, 邹秉章, 王思荣, 万晓华. 亚热带人工幼林土壤呼吸与植物功能性状的关系 [J]. 应用生态学报, 2023, 34(11): 2898-2906.
[5]	赵雷, 靳海笛, 曹晓云, 邓文辉, 杜灵娟. 六个东方铁筷子品种对干旱胁迫的生理响应及抗旱性评价 [J]. 应用生态学报, 2023, 34(10): 2644-2654.
[6]	时一平, 宋彦涛, 那木汗, 彭擎天, 吕林有, 申悦, 倩娜, 乌云娜. 羊草和紫花苜蓿人工草地光合特性 [J]. 应用生态学报, 2023, 34(10): 2655-2662.
[7]	朱静, 金星, 何中声, 肖倩茹, 陈佳嘉, 邢聪, 刘金福, 沈彩霞. 格氏栲幼苗叶绿素荧光和非结构性碳水化合物积累对种子散布位置的响应 [J]. 应用生态学报, 2022, 33(8): 2129-2138.
[8]	翟培凤, 关家欣, 何鹏, 刘贺永, 满良, 姜勇, 马成仓. 沿干旱梯度樟子松人工林针叶和枝条非结构性碳水化合物及氮含量的变化 [J]. 应用生态学报, 2022, 33(6): 1518-1524.
[9]	刘梅朔, 王传宽, 全先奎. 兴安落叶松叶光合与氮代谢对环境变化响应的转录组分析 [J]. 应用生态学报, 2022, 33(4): 957-962.
[10]	李月灵, 金则新, 罗光宇, 陈超, 孙中帅, 王晓燕. 干旱胁迫下接种丛枝菌根真菌对七子花非结构性碳水化合物积累及C、N、P化学计量特征的影响 [J]. 应用生态学报, 2022, 33(4): 963-971.
[11]	郑悦, 王爱英, 苏立新, 郭晶晶, 段春旸, 殷笑寒, 龚雪伟, 郝广友. 沈阳市区不同环境下银杏水力特征和非结构性碳水化合物含量 [J]. 应用生态学报, 2022, 33(3): 711-719.
[12]	周光, 徐玮泽, 万静, 汪雁楠, 刘丽婷, 刘琪璟. 长白山阔叶红松林不同演替阶段林下红松幼苗能量与养分季节动态 [J]. 应用生态学报, 2021, 32(5): 1663-1672.
[13]	方笑堃, 陈志炜, 程兆康, 姜海波, 邱丹, 罗小三. 太阳辐射减弱对水稻光合生理特性和中微量元素积累的影响 [J]. 应用生态学报, 2021, 32(4): 1345-1351.
[14]	吴龙龙, 田仓, 张露, 黄晶, 朱练峰, 张均华, 曹小闯, 金千瑜. 稻田水氮氧环境因子对水稻生长发育、光合作用和氮利用的调控研究进展 [J]. 应用生态学报, 2021, 32(4): 1498-1508.
[15]	韦小练, 范泽鑫, Arisa Kaewmano, 林友兴, 陈礼敏, 付培立. 热带季节雨林多花白头树年内径向生长动态及其对环境因子的响应 [J]. 应用生态学报, 2021, 32(10): 3567-3575.