欢迎访问《应用生态学报》官方网站,今天是 分享到:

应用生态学报 ›› 2017, Vol. 28 ›› Issue (9): 2840-2848.doi: 10.13287/j.1001-9332.201709.032

• 目次 • 上一篇    下一篇

高山林线土壤微生物群落结构对模拟增温的响应

郑海峰, 陈亚梅, 杨林, 李洪杰, 王利峰, 谌贤, 刘洋*   

  1. 高山森林生态系统定位研究站/四川省长江上游林业生态工程重点实验室/四川农业大学生态林业研究所, 成都 611300
  • 收稿日期:2017-03-21 出版日期:2017-09-18 发布日期:2017-09-18
  • 通讯作者: * E-mail: sicauliuyang@163.com
  • 作者简介:郑海峰, 男, 1992年生, 硕士研究生. 主要从事高山森林土壤生态学研究. E-mail: 791561291@qq.com
  • 基金资助:

    本文由国家自然科学基金项目(31570605,31200345)资助

Responses of soil microbial community structure to simulated warming in alpine timberline in western Sichuan, China.

ZHENG Hai-feng, CHEN Ya-mei, YANG Lin, LI Hong-jie, WANG Li-feng, SHEN Xian, LIU Yang*   

  1. Long-term Research Station of Alpine Forest Ecosystems/Sichuan Province Key Laboratory of Ecological Forestry Engineering/Institute of Ecology & Forest, Sichuan Agricultural University, Chengdu 611130, China.
  • Received:2017-03-21 Online:2017-09-18 Published:2017-09-18
  • Contact: * E-mail: sicauliuyang@163.com
  • Supported by:

    This work was supported by the National Natural Science Foundation of China (31570605, 31200345).

摘要: 研究土壤微生物群落结构对模拟增温的响应,对预测全球气候变化背景下土壤碳氮磷循环具有重要意义.采用开顶式生长室(OTC)模拟增温,研究了土壤有机质层和矿质土壤层真菌(F)、细菌(B)、革兰氏阳性菌(G+)和革兰氏阴性菌(G-)PLFAs微生物量,以及真菌/细菌(F/B)和革兰氏阴性菌/革兰氏阳性菌(G-/G+)比值对模拟增温的响应.结果表明: OTC模拟增温使空气温度增加0.87 ℃,土壤有机质层温度增加0.5 ℃,矿质土壤层温度增加0.23 ℃.土壤有机质层微生物群落组成比矿质土壤层对模拟增温的响应更敏感.细菌比真菌对模拟增温的响应更加敏感,模拟增温显著影响了土壤有机质层的F/B和G-/G+比值,对矿质土壤层的所有PLFAs含量或比值均没有显著影响.微生物的PLFAs含量及真菌/细菌和G-/G+比值总体呈现非生长季低于生长季前期和生长季后期.冗余分析表明,土壤中的碳含量(可溶性有机碳DOC 12.1%、凋落物可溶性碳DC 9.5%和全碳TC 3%)是微生物群落结构的决定性因素,可溶性组分(DOC和DC)对微生物群落结构的影响大于全量养分(全碳和总氮).

Abstract: Studying the response of soil microbial community structure to simulate warming is of great significance for predicting soil carbon, nitrogen and phosphorus cycles in the context of global climate change. Therefore, the effect of simulated warming on the soil microbial community structure was studied with the open-top chamber (OTC), as determined by phospholipid fatty acids (PLFAs) in the alpine timberline ecosystem in western Sichuan. The results showed that simulated warming enhanced the daily mean air temperature by 0.87 ℃, the daily mean temperature of soil organic layer (OL) and mineral soil layer (ML) increased 0.5 ℃ and 0.23 ℃, respectively. The microbial community composition of soil organic layer was more sensitive to the simulated warming than that of the mineral soil layer. Bacteria (TB) were more sensitive to the simulated warming than fungi (TF). Simulated warming also significantly affected the ratios of fungi to bacteria (F/B) and gram-negative bacteria to Gram-positive bacteria (G-/G+) of the soil organic layer, whereas it had no significant effect on the microbial PLFAs content of mineral soil layer. In all treatments, the content of microbial PLFAs and the ratios of F/B and G-/G+ were in order of non-growing season (NGS) < early growing season (EGS) < late growing season (LGS). The redundancy analysis suggested that the soil carbon contents (DOC 12.1%, DC 9.5% and TC 3%) were the determination factors affecting microbial community structure, and the dissolved nutrient (DOC and DC) had a greater effect on microbial community structure than the total nutrient (TC and TN).