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应用生态学报 ›› 2017, Vol. 28 ›› Issue (2): 519-527.doi: 10.13287/j.1001-9332.201702.028

• 中国生态学学会2016年学术年会会议专栏 • 上一篇    下一篇

川西亚高山不同林龄云杉人工林土壤微生物群落结构

罗达1, 2, 刘顺1, 史作民1, 3*, 冯秋红4, 刘千里5, 张利5, 黄泉5, 何建社5   

  1. 1中国林业科学研究院森林生态环境与保护研究所, 国家林业局森林生态环境重点实验室, 北京100091;
    2新疆林业科学院经济林研究所, 乌鲁木齐 830063;
    3南京林业大学南方现代林业协同创新中心, 南京 210037;
    4四川省林业科学研究院, 成都 610081;
    5阿坝州林业科学技术研究所, 四川汶川 623000
  • 收稿日期:2016-06-10 出版日期:2017-02-18 发布日期:2017-02-18
  • 通讯作者: * E-mail: shizm@caf.ac.cn
  • 作者简介:罗达, 男, 1984年生, 博士. 主要从事森林土壤碳氮循环关键过程研究. E-mail: luoda2010@163.com
  • 基金资助:
    本文由中央级公益性科研院所基本科研业务费专项(CAFYBB2014MA004)和国家重点研发计划项目(2016YFC0502104-02)资助

Soil microbial community structure in Picea asperata plantations with different ages in subalpine of western Sichuan, Southwest China.

LUO Da1, 2, LIU Shun1, SHI Zuo-min1, 3*, FENG Qiu-hong4, LIU Qian-li5, ZHANG Li5, HUANG Quan5, HE Jian-she5   

  1. 1State Forestry Administration Key Laboratory on Forest Ecology and Environmental Sciences, Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China;
    2Research Institute of Economic Forestry, Xinjiang Academy of Forestry Science, Urumqi 830063, China;
    3Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China;
    4Sichuan Academy of Forestry, Chengdu 610081, China;
    5Aba Autonomous Prefecture Forestry of Science and Technology, Wenchuan 623000, Sichuan, China.

  • Received:2016-06-10 Online:2017-02-18 Published:2017-02-18
  • Contact: * E-mail: shizm@caf.ac.cn
  • Supported by:
    This work was supported by the National Nonprofit Institute Research Grant of Chinese Academy of Forestry (CAFYBB2014MA004), and the National Key Research and Development Program (2016YFC0502104-02).

摘要: 以川西亚高山云杉人工林林地土壤为对象,采用磷脂脂肪酸(PLFA)法研究了4种不同林龄(50、38、27和20年)的人工林土壤微生物多样性和群落结构特征.结果表明: 随着林龄的增加,土壤有机碳和全氮含量逐步增加;土壤微生物Shannon多样性和Pielou均匀度指数则呈现先增后减的趋势.土壤微生物总PLFAs量、细菌PLFAs量、真菌PLFAs量、放线菌PLFAs量以及丛枝菌根真菌PLFAs量均表现为随林龄的增加而增加.主成分分析(PCA)表明,不同林龄人工林的土壤微生物群落结构之间存在显著差异,其中,第1主成分(PC1)和第2主成分(PC2)共同解释了土壤微生物群落结构总变异的66.8%.冗余分析(RDA)表明,对土壤微生物群落结构产生显著影响的环境因子分别为土壤有机碳、全氮、全钾以及细根生物量.随着人工造林时间的延长,土壤肥力和微生物生物量增加,森林生态系统的恢复进程稳定.

Abstract: The effects of four Picea asperata plantations with different ages (50-, 38-, 27- and 20-year-old), in subalpine of western Sichuan, on the characteristics of soil microbial diversity and microbial community structure were studied by the method of phospholipid fatty acid (PLFA) profiles. The results showed that, with the increase of age, the contents of soil organic carbon and total nitrogen gradually improved, while Shannon’s diversity index and Pielou’s evenness index of soil microorganisms increased at first and then decreased. The amounts of microbial total PLFAs, bacterial PLFAs, fungal PLFAs, actinobacterial PLFAs, and arbuscular mycorrhizal fungal (AMF) PLFAs in soils consistently increased with increasing age. The principal component analysis (PCA) indicated that the soil microbial communities in different plantations were structurally distinct from each other. The first principal component (PC1) and the second principal component (PC2) together accounted for 66.8% of total variation of the soil microbial community structure. The redundancy analysis (RDA) of soil microbial community structure and environmental factors showed that soil organic carbon, total nitrogen, total potassium, and fine root mass were the key determinants influencing the microbial community structure. Our study suggested that, with the extension of artificialafforestation time, the soil fertility and microbial biomass were enhanced, and the restoration processes of forest ecosystem were stable.