不同光脉冲方案对光下最大荧光参数及其计算参数的影响

doi:10.13287/j.1001-9332.201704.025

应用生态学报 ›› 2017, Vol. 28 ›› Issue (4): 1137-1144.doi: 10.13287/j.1001-9332.201704.025

不同光脉冲方案对光下最大荧光参数及其计算参数的影响

唐星林^1,2, 曹永慧^1,2, 周本智^1,2*, 周燕³, 顾连宏⁴

¹中国林业科学研究院亚热带林业研究所, 杭州 311400
²国家林业局钱江源森林生态系统定位观测研究站, 杭州 311400
³建德市新安江林场, 杭州 311600
⁴美国橡树岭国家实验室气候变化研究所, 橡树岭, 田纳西州 37831, 美国

收稿日期:2016-10-13 出版日期:2017-04-18 发布日期:2017-04-18
通讯作者: * E-mail: benzhi_zhou@126.com
作者简介:唐星林,男,1988年生,博士研究生.主要从事森林生态学研究.E-mail:txl_insist@163.com
基金资助:
本文由国家重点基础研究发展计划项目(2016YFD0600202)、国家林业局948项目(2014-4-57)、浙江省自然科学基金项目(LY13C160002)、中央级公益性科研院所基本科研业务费专项(RISF2013002)和中国林业科学研究院基本科研业务费专项资金项目——海内外杰出学者讲习计划(CAFYBB2011007)资助

Effects of flash scheme on maximum chlorophyll fluorescence under illumination and its derived parameters

TANG Xing-lin^1,2, CAO Yong-hui^1,2, ZHOU Ben-zhi^1,2*, ZHOU Yan³, GU Lian-hong⁴

¹Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China
²Qianjiangyuan Forest Ecosystem Research Station, State Forestry Administration, Hangzhou 311400, China
³Xin’anjiang Forest Center of Jiande, Hangzhou 311600, China
⁴Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA

Received:2016-10-13 Online:2017-04-18 Published:2017-04-18
Contact: * E-mail: benzhi_zhou@126.com
Supported by:
This work was supported by the National Key Research and Development Program of China (2016YFD0600202), the 948 Importing Program of State Forestry Administration (2014-4-57), the Natural Science Foundation of Zhejiang Province, China (LY13C160002), the Special Fund for Scientific Research in the Central Public-interest Research Institutes (RISF2013002) and the Special Fund for Basis Research of Chinese Academy of Forestry: Lecture and Study Program for Outstanding Scholars from Home and Abroad (CAFYBB2011007)

摘要/Abstract

摘要： 光下最大荧光(F_m′)是植物生理生态研究中的重要参数,一般采用饱和脉冲(RF)方案来估计.然而,光系统Ⅱ(PSⅡ)受体库的反馈调节会影响RF方案对F_m′估计的准确性.为消除PSⅡ受体库反馈调节的影响,根据光脉冲强度(Q′)与叶绿素荧光(F′)的线性关系提出多相脉冲(MPF)方案,估算Q′无穷大时的F′(即F_m′).本研究采用MPF和RF方案分别对苦槠、青冈和乌桕3个树种叶片的叶绿素荧光和气体交换数据进行同步测量,并对两种方案估计的F_m′及其计算参数PSⅡ光化学效率(Φ_PSII)、PSⅡ的电子传递速率(J)、最大电子传递速率(J_max)、叶肉导度(g_m)和叶绿体内CO₂浓度(C_c)等光合参数进行比较,分析两种方案对3个树种叶片6个光合参数的影响.结果表明: 当光合有效辐射(PAR)<200 μmol·m^-2·s^-1时,两种方案对苦槠、青冈和乌桕叶片F_m′、Φ_PSII和J的估计无显著影响;当PAR>200 μmol·m^-2·s^-1时,采用MPF方案获得的苦槠、青冈和乌桕的F_m′分别比RF方案获得的F_m′高3.5%～5.2%、11.7%～18.0%和3.2%～7.1%;当PAR>200 μmol·m^-2·s^-1时,采用MPF方案获得的Φ_PSII、J和J_max分别不同程度地大于RF方案获得的参数,g_m和C_c分别不同程度地小于RF方案获得的参数.说明当PAR较低(<200μmol·m^-2·s^-1)时,MPF与RF方案对植物叶片F_m′、Φ_PSII、J的估计没有显著影响;当PAR较高(≥200μmol·m^-2·s^-1)时,MPF与RF方案对植物叶片F_m′、Φ_PSII、J、J_max、g_m和C_c的估计有显著影响,且RF方案对植物叶片的F_m′、Φ_PSII、J和J_max比MPF方案分别有不同程度的低估,对g_m和C_c则有不同程度的高估.

Abstract: The maximum chlorophyll fluorescence yield under illumination (F_m′) is one of the most important parameters in plant eco-physiological research, and usually was estimated with rectangular flush scheme (RF). However, the estimation accuracy of RF for F_m′ was affected by the rapid turnover of photosystem Ⅱ (PSⅡ). In order to eliminate the effect of the rapid turnover of photosystem Ⅱ (PSⅡ), the multiphase flush scheme (MPF) based on the linear relationship between the flush (Q′) and chlorophyll fluorescence (F′) is proposed to estimate F_m′ at infinite irradiance. Leaf gas exchange and chlorophyll fluorescence of three woody species (Castanopsis sclerophylla, Cyclobalanopsis glauca, and Sapium sebiferum) were respectively measured with RF and MPF, F_m′ and the derived parameters [the quantum efficiency of PSⅡ (Φ_PSII), the electron flux through PSⅡ (J), the maximum electron transfer rate (J_max), mesophyll conductance (g_m) and chloroplast CO₂ concentration (C_c)] were compared between the two different schemes, and the effects of RF and MPF on these parameters were analyzed. The results showed that no significant difference was found in the parameters for the three species between RF and MPF at the light intensity lower than 200 μmol·m^-2·s^-1. F_m′ estimated with MPF for the three species were 3.5%-5.2%, 11.7%-18.0%, and 3.2%-7.1% higher than those with RF, respectively, at the light intensity higher than 200 μmol·m^-2·s^-1. The derived parameters (Φ_PSII, J and J_max) estimated with MPF for the three species were higher than those with RF, while the derived parameters(g_m and C_c) estimated with MPF were lower at the light intensity higher than 200 μmol·m^-2·s^-1. In conclusion, estimates of parameters (F_m′, Φ_PSII, and J) were not significantly affected by the two different schemes at the light intensity lower than 200 μmol·m^-2·s^-1. The estimates of parameters (F_m′, Φ_PSII, J, J_max, g_m, and C_c) were significantly affected by the two different schemes at the light intensity higher than 200 μmol·m^-2·s^-1. Compared with MPF, parameters of F_m′, Φ_PSII, J and J_max estimated with RF were underestimated, while parameters of g_m and C_c were overestimated.

唐星林, 曹永慧, 周本智, 周燕, 顾连宏. 不同光脉冲方案对光下最大荧光参数及其计算参数的影响[J]. 应用生态学报, 2017, 28(4): 1137-1144.

TANG Xing-lin, CAO Yong-hui, ZHOU Ben-zhi, ZHOU Yan, GU Lian-hong. Effects of flash scheme on maximum chlorophyll fluorescence under illumination and its derived parameters[J]. Chinese Journal of Applied Ecology, 2017, 28(4): 1137-1144.

参考文献

[1] Perezmartin A, Michelazzo C, Torresruiz JM, et al. Regulation of photosynthesis and stomatal and mesophyll conductance under water stress and recovery in olive trees: Correlation with gene expression of carbonic anhydrase and aquaporins. Journal of Experimental Botany, 2014, 65: 3143-3156
[2] Xiong D, Liu X, Liu L, et al. Rapid responses of mesophyll conductance to changes of CO₂ concentration, temperature and irradiance are affected by N supplements in rice. Plant, Cell and Environment, 2015, 38: 2541-2550
[3] Xu D-Q (许大全). Photosynthesis. Beijing: Science Press, 2013 (in Chinese)
[4] Wu G-L (吴甘霖), Duan R-Y (段仁燕), Wang Z-G (王志高), et al. Effects of drought stress and rehydration on chlorophyll fluorescence characteristics in Fragaria ananassa Duch. Acta Ecologica Sinica (生态学报), 2010, 30(14): 3941-3946 (in Chinese)
[5] Song Y-Z (宋玉芝), Cai W (蔡炜), Qin B-Q (秦伯强). Photosynthetic fluorescence characteristics of floating-leaved and submersed macrophytes commonly found in Taihu Lake. Chinese Journal of Applied Ecology (应用生态学报), 2009, 20(3): 569-573 (in Chinese)
[6] Long SP, Bernacchi CJ. Gas exchange measurements, what can they tell us about the underlying limitations to photosynthesis? Procedures and sources of error. Journal of Experimental Botany, 2003, 54: 2393-2401
[7] Lal A, Ku MSB, Edwards GE. Analysis of inhibition of photosynthesis due to water stress in the C₃ species Hordeum vulgare and Vicia faba: Electron transport, CO₂ fixation and carboxylation capacity. Photosynthesis Research, 1996, 49: 57-69
[8] Valentini R, Epron D, Angelis P, et al. In situ estimation of net CO₂ assimilation, photosynthetic electron flow and photorespiration in Turkey oak (Q. cerris L.) lea-ves: Diurnal cycles under different levels of water supply. Plant, Cell and Environment, 1995, 18: 631-640
[9] Harley PC, Loreto F, Marco GD, et al. Theoretical considerations when estimating the mesophyll conductance to CO₂ flux by analysis of the response of photosynthesis to CO₂. Plant Physiology, 1992, 98: 1429-1436
[10] Loreto F, Harley PC, Marco GD, et al. Estimation of mesophyll conductance to CO₂ flux by three different methods. Plant Physiology, 1992, 98: 1437-1443
[11] Ögren E, Baker NR. Evaluation of a technique for the measurement of chlorophyll fluorescence from leaves exposed to continuous white light. Plant, Cell and Environment, 1985, 8: 539-547
[12] Schreiber U, Schliwa U, Bilger W. Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosynthesis Research, 1986, 10: 51-62
[13] Schreiber U. Pulse-Amplitude-Modulation (PAM) Fluorometry and Saturation Pulse Method: An Overview. Dordrecht: Springer, 2004
[14] Markgraf T, Berry J. Measurement of Photochemical and Non-photochemical Quenching: Correction for Turnover of PS2 during Steady-state Photosynthesis.Dordrecht: Kluwer Academic Publishers, 1990
[15] Loriaux SD, Avenson TJ, Welles JM, et al. Closing in on maximum yield of chlorophyll fluorescence using a single multiphase flash of sub-saturating intensity. Plant, Cell and Environment, 2013, 36: 1755-1770
[16] Earl HJ, Ennahli S. Estimating photosynthetic electron transport via chlorophyll fluorometry without photosystem Ⅱ light saturation. Photosynthesis Research, 2004, 82: 177-186
[17] Yang X-H (杨兴洪), Zou Q (邹琦), Zhao S-J (赵世杰). Photosynthetic characteristics and chlorophyll fluorescence in leaves of cotton plants grown in full light and 40% sunlight. Acta Phytoecologica Sinica (植物生态学报), 2005, 29(1): 8-15 (in Chinese)
[18] Sun G-C (孙谷畴), Zhao P (赵平). Responses of mesophyllic conductance in leaves of 4 dominant subtropical forest tree species to moderate high temperature. Chinese Journal of Applied Ecology (应用生态学报), 2007, 18(6): 1187-1193 (in Chinese)
[19] Cano FJ, López R, Warren CR. Implications of the mesophyll conductance to CO₂ for photosynthesis and water-use efficiency during long-term water stress and recovery in two contrasting Eucalyptus species. Plant, Cell and Environment, 2014, 37: 2470-2490
[20] Théroux-Rancourt G, thier G, Pepin S. Threshold response of mesophyll CO₂ conductance to leaf hydraulics in highly transpiring hybrid poplar clones exposed to soil drying. Journal of Experimental Botany, 2014, 65: 741-753
[21] Genty B, Briantais JM, Baker NR. The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochi-mica et Biophysica Acta, 1989, 990: 87-92
[22] Major KM, Dunton KH. Variations in light-harvesting characteristics of the seagrass, Thalassia testudinum: Evidence for photoacclimation. Journal of Experimental Marine Biology and Ecology, 2002, 275: 173-189
[23] von Caemmerer S. Biochemical Models of Leaf Photosynthesis. Collingwood: CSIRO Publishing, 2000
[24] Walker BJ, SkabelundDC, Busch FA, et al. An improved approach for measuring the impact of multiple CO₂ conductances on the apparent photorespiratory CO₂ compensation point through slope-intercept regression. Plant, Cell and Environment, 2016, 39: 1198-1203
[25] Gu L, Sun Y. Artefactual responses of mesophyll conductance to CO₂ and irradiance estimated with the variable J and online isotope discrimination methods. Plant, Cell and Environment, 2014, 37: 1231-1249

不同光脉冲方案对光下最大荧光参数及其计算参数的影响

Effects of flash scheme on maximum chlorophyll fluorescence under illumination and its derived parameters

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价