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应用生态学报 ›› 2018, Vol. 29 ›› Issue (1): 231-237.doi: 10.13287/j.1001-9332.201801.027

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对羟基苯甲酸和间苯三酚对棉花幼苗根系线粒体功能和根系生长的影响

张国伟, 杨长琴, 刘瑞显*, 倪万潮   

  1. 江苏省农业科学院经济作物研究所/农业部长江下游棉花与油菜重点实验室, 南京 210014
  • 收稿日期:2017-03-22 出版日期:2018-01-18 发布日期:2018-01-18
  • 通讯作者: * E-mail: liuruixian2008@163.com
  • 作者简介:张国伟,男,1981年生,副研究员.主要从事棉花栽培生理研究.E-mail: zgw_0721@163.com
  • 基金资助:
    本文由国家重点研发计划项目(2017YFD0201900)和农业部长江下游棉花与油菜重点实验室开放课题(2017-CR01)资助

Effects of p-hydroxybenzoic acid and phloroglucinol on mitochondria function and root growth in cotton (Gossypium hirsutum L.) seedling roots.

ZHANG Guo-wei, YANG Chang-qin, LIU Rui-xian*, NI Wan-chao   

  1. Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Cotton and Rapeseed in the Lower Reaches of the Yangtze River, Ministryof Agriculture, Nanjing 210014, China
  • Received:2017-03-22 Online:2018-01-18 Published:2018-01-18
  • Contact: * E-mail: liuruixian2008@163.com
  • Supported by:
    This work was supported by the National Key Research and Development Plan of China (2017YFD0201900) and the Open Fund from Key Laboratory of Cotton and Rapeseed in the Lower Reaches of the Yangtze River, Ministry of Agriculture (2017-CR01).

摘要: 以中国大面积种植的早熟棉中棉所50(CCRI-50)为材料,设置水培试验,研究不同浓度(0.8、4.0、20.0 mmol·L-1)的对羟基苯甲酸和间苯三酚对棉花苗期根系线粒体活性氧产生、抗氧化酶活性变化及线粒体特性的影响.结果表明: 对羟基苯甲酸和间苯三酚抑制了棉花根系生长,降低了根系线粒体超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)和线粒体膜H+-ATPase活性,增加了O2产生速率和H2O2含量;对羟基苯甲酸和间苯三酚处理还增大了线粒体细胞通透性转换孔(MPTP)开放程度,降低了线粒体膜流动性和细胞色素Cyt c/a 值.0.8 mmol·L<sup>-1的对羟基苯甲酸和间苯三酚处理间线粒体功能差异相对较小,4.0和20.0 mmol·L-1时,对羟基苯甲酸处理对根系生长和线粒体功能的抑制作用高于间苯三酚处理.总之,对羟基苯甲酸和间苯三酚均抑制了棉苗根系生长和线粒体功能,且浓度越高,抑制作用越强.对羟基苯甲酸和间苯三酚处理间存在差异,浓度高于4.0 mmol·L-1时,对羟基苯甲酸比间苯三酚具有更强的抑制作用.

Abstract: With early-maturing cotton cultivar CCRI-50 widely grown in China as experimental material, water culture experiment was conducted to study the effects of p-hydroxybenzoic acid and phloroglucinol with different concentrations (0.8, 4.0, and 20.0 mmol·L-1) on generation rate of reactive oxygen, changes of antioxidant enzyme activities and mitochondria function of cotton roots. Results showed that p-hydroxybenzoic acid and phloroglucinol treatments inhibited the cotton root growth, reduced SOD, POD, CAT and H+-ATPase activities in root mitochondria, increased the generation rate of O2-· and H2O2 content. In addition, they also increased the opening of mitochondrial permeability transition pores (MPTP), decreased the membrane fluidity and cytochrome c/a (Cyt c/a). Difference of mitochondria function between p-hydroxybenzoic acid and phloroglucinol treatments was minor at concentration of 0.8 mmol·L-1, while the inhibition to root growth and mitochondria function under treatment of p-hydroxybenzoic acid at concentration of 4.0 and 20.0 mmol·L-1 was stronger than that of phloroglucinol. Above all, p-hydroxybenzoic acid and phloroglucinol inhibited antioxidant enzyme activity and mitochondrial function in cotton seedling roots, and the inhibition depended on dose of phenolic acids. The inhibition to root growth and mitochondria function between p-hydroxybenzoic acid and phloroglucinol treatment was different, and p-hydroxybenzoic acid had stronger inhibition than phloroglucinol at the concentration more than 4.0 mmol·L-1.