稻田土壤有机碳矿化及其激发效应对磷添加的响应

doi:10.13287/j.1001-9332.201803.023

应用生态学报 ›› 2018, Vol. 29 ›› Issue (3): 857-864.doi: 10.13287/j.1001-9332.201803.023

稻田土壤有机碳矿化及其激发效应对磷添加的响应

唐美玲^1,2, 魏亮², 祝贞科², 李欢², 周萍², 葛体达², 吴金水², 王光军^1*

¹中南林业科技大学生命科学与技术学院,长沙 410004;
²中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 长沙 410125

收稿日期:2017-05-18 出版日期:2018-03-18 发布日期:2018-03-18
通讯作者: * E-mail: wanggj652@163.com
作者简介:唐美玲,女,1993年生,硕士研究生. 主要从事稻田土壤碳循环的研究. E-mail: 3193067323@qq.com
基金资助:
本文由国家自然科学基金项目(41430860,41371304)、中国科学院战略性先导科技专项(XDB15020401)和湖南省自然科学基金项目(2016JJ3132)资助

Responses of organic carbon mineralization and priming effect to phosphorus addition in paddy soils.

TANG Mei-ling^1,2, WEI Liang², ZHU Zhen-ke², LI Huan², ZHOU Ping², GE Ti-da², WU Jin-shui² , WANG Guang-jun^1*

¹College of Life Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China;
²Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China

Received:2017-05-18 Online:2018-03-18 Published:2018-03-18
Contact: * E-mail: wanggj652@163.com
Supported by:
This work was supported by the National Natural Science Foundation of China (41430860, 41371304), the Special Sub-Subject of Strategic Leading Science and Technology of Chinese Academy of Sciences (XDB15020401), and the Natural Science Foundation of Hunan Province (2016JJ3132)

摘要/Abstract

摘要： 采用室内模拟培养和¹³C同位素标记技术相结合的研究方法,探讨了在葡萄糖与无机氮肥共施的条件下,土壤有机碳矿化及其激发效应对外源磷添加的响应,以揭示土壤有机碳矿化的碳磷耦合调控机制.结果表明: 外源磷的输入加快了CO₂的释放,但抑制了CH₄的释放;在整个土壤淹水培养期间,磷添加抑制了土壤碳矿化释放CH₄总量的53.1%,其中外源葡萄糖-¹³C矿化成¹³CH₄的总量降低了70.5%;磷添加促使通过微生物转化的葡萄糖-¹³C向易利用态碳库的分配比例增加了3.6%,显著提高土壤有机碳快库矿化速率,缩短土壤碳矿化周期.土壤培养前期,外源有机质的添加表现为短暂的负激发效应;随着葡萄糖不断矿化分解, CO₂累积激发效应(PE_CO₂)总体上呈现先增加后下降的趋势,而CH₄累积激发效应(PE_CH₄)稳步增加最终保持基本稳定状态;培养结束时(100 d),在磷添加条件下,PE_CO₂增强32.3%,PE_CH₄显著降低93.4%.冗余分析和Pearson分析表明,电导率、氧化还原电位和溶解有机碳对稻田土壤碳矿化的影响最为显著;速效磷与¹³CH₄、PE_CH₄呈极显著负相关.在外源有机质添加条件下,磷的添加能够抑制CH₄排放及其激发效应,促进土壤有机质的矿化和养分释放,提高土壤原有有机碳的可利用性,促进稻田土壤有机碳循环.

关键词: 稻田土, 磷添加, 有机碳矿化, 激发效应

Abstract: To understand the coupled controlling of carbon (C) and phosphorus (P) on the minera-lization of soil organic carbon and amended substrates in paddy soil, we investigated the effects of P addition on the decomposition of organic carbon and its induced priming effect by using ¹³C isotope probing technique in microcosm. The results showed that P addition accelerated the release of CO₂ but inhibited the release of CH₄, leading to 53.1% reduction of total accumulated CH₄ and 70.5% reduction of the ¹³CH₄ derived from exotic glucose-¹³C. P addition altered the carbon distribution during the microbial turnover progress, with 3.6% of glucose-¹³C being transferred into the labile carbon pool, therein significantly increased potential of the mineralization rate of exogenous C. A transient negative priming effect was observed in the early stage of incubation. With time prolonging, the priming effect on CO₂ emission (PE_CO₂) generally increased and then decreased after a peak. The priming effect on CH₄ emission (PE_CH₄) kept increasing and finally fluctuated at a relative stable value until the end of the experiment (100 days). P addition increased PE_CO₂ by 32.3% but reduced PE_CH₄ by 93.4%. Results from the RDA and Pearson analysis showed that electric conductivity, oxidation-reduction potential and dissolved organic carbon significantly affected soil C mineralization. There were significantly negative correlations between available phosphorus (Olsen-P) and ¹³CH₄, and between Olsen-P and PE_CH₄. In conclusion, with the addition of exogenous organic matter, P application could reduce CH₄ emissions and inhibit its priming effect, acce-lerate the mineralization of SOC, probably improve the nutrient supply, and thus enhance the avai-lability of organic C and promote C cycling in paddy soil.

Key words: paddy soil, priming effect, organic carbon mineralization, phosphorus addition

唐美玲, 魏亮, 祝贞科, 李欢, 周萍, 葛体达, 吴金水, 王光军. 稻田土壤有机碳矿化及其激发效应对磷添加的响应[J]. 应用生态学报, 2018, 29(3): 857-864.

TANG Mei-ling, WEI Liang, ZHU Zhen-ke, LI Huan, ZHOU Ping, GE Ti-da, WU Jin-shui,WANG Guang-jun. Responses of organic carbon mineralization and priming effect to phosphorus addition in paddy soils.[J]. Chinese Journal of Applied Ecology, 2018, 29(3): 857-864.

参考文献

[1] Lao X-R (劳秀荣), Wu Z-Y (吴子一), Gao Y-C (高燕春). Effect of long-term returning straw to soil fertility. Transactions of the Chinese Society of Agricultural Engineering (农业工程学报), 2002, 18(2): 49-52 (in Chinese)
[2] Sun L-Y (孙丽英). Atmospheric Nitrogen and Phosphorus Deposition in Nanjing and Effects of Simulated Nitrogen Deposition on Gaseous Emissions of Soils. PhD Thesis. Nanjing: Nanjing Agricultural University, 2014 (in Chinese)
[3] Kuzyakov Y, Friedel JK, Stahr K. Review of mechanisms and quantification of priming effects. Soil Biology and Biochemistry, 2000, 32: 1485-1498
[4] Wang H, Xu WH, Hu GQ, et al. The priming effect of soluble carbon inputs in organic and mineral soils from a temperate forest. Oecologia, 2015, 178: 1239-1250
[5] Zhu P-L (朱培立), Huang D-M (黄东迈). Discussion on priming effect of soil nitrogen. Scientia Agricultura Sinica (中国农业科学), 1994, 27(4): 45-52 (in Chinese)
[6] Tian Y-W (田耀武), Wang N (王宁), Liu J (刘晶). The priming effect of soil organic carbon induced by nustedge. Journal of Nuclear Agricultural Sciences (核农学报), 2016, 30(12): 2418-2424 (in Chinese)
[7] Abel S, Ticconi CA, Delatorre CA. Phosphate sensing in higher plants. Physiologia Plantarum, 2010, 115: 1-8
[8] Zhao Q (赵琼), Zeng D-H (曾德慧). Phosphorus cycling in terrestrial ecosystems and its controlling factors. Acta Phytoecologica Sinica (植物生态学报), 2005, 29(1): 153-163 (in Chinese)
[9] Wang Y-Z (王永状), Chen X (陈欣), Shi Y (史奕). Phosphorus availability in cropland soils of China and related affecting factors. Chinese Journal of Applied Ecology (应用生态学报), 2013, 24(1): 260-268 (in Chinese)
[10] Zhou QX, Zhu YM. Potential pollution and recommended critical levels of phosphorus in paddy soils of the southern Lake Tai area, China. Geoderma, 2003, 115: 45-54
[11] Turner BL, Lambers H, Condron LM, et al. Soil microbial biomass and the fate of phosphorus during long-term ecosystem development. Plant and Soil, 2013, 367: 225-234
[12] Huang M (黄敏). Variation Characteristics and Driving Factors of Soil Organic Carbon and Phosphorus in Hilly Regions of Subtropical China. PhD Thesis. Wuhan: Huazhong Agricultural University, 2005 (in Chinese)
[13] Wang J-L (王建林), Zhong Z-M (钟志明), Wang Z-H (王忠红), et al. Soil C/P distribution characteristics of alpine steppe ecosystems in the Qinghai-Tibetan Plateau. Acta Pratacultura Sinica (草业学报), 2014, 23(2): 9-19 (in Chinese)
[14] Creamer CA, Jones DL, Baldock JA, et al. Stoichiome-tric controls upon low molecular weight carbon decomposition. Soil Biology and Biochemistry, 2014, 79: 50-56
[15] Sinsabaugh RL, Manzoni S, Moorhead DL, et al. Carbon use efficiency of microbial communities: Stoichiometry, methodology and modeling. Ecology Letters, 2013, 16: 930-939
[16] Chen X-Y (陈小云), Guo J-H (郭菊花), Liu M-Q (刘满强), et al. Effects of fertilization on lability and recalcitrancy of organic carbon of red paddy soils. Acta Pedologica Sinica (土壤学报), 2011, 48(1): 125-131 (in Chinese)
[17] Liu D-Y (刘德燕), Song C-C (宋长春). Effects of phosphorus enrichment on mineralization of organic and contents of dissolved carbon in a freshwater marsh soil. China Environmental Science (中国环境科学), 2008, 28(9): 769-774 (in Chinese)
[18] Li X (李霞), Tian G-M (田光明), Zhu J (朱军), et al. Effects of rate of phosphorus fertilizer on organic carbon mineralization and bacterial community diversity in paddy soil. Acta Pedologica Sinica (土壤学报), 2014, 51(2): 360-372 (in Chinese)
[19] Liu Y (刘洋). Effects of Nitrogen and Phosphorus Addition on Soil Organic Carbon in Sub-alpine Meadows of Qinghai-Tibetan Plateau. Master Thesis. Lanzhou: Lanzhou University, 2014 (in Chinese)
[20] Keller JK, Bridgham SD, Chapin CT, et al. Limited effects of six years of fertilization on carbon mineralization dynamics in a Minnesota fen. Soil Biology and Biochemistry, 2005, 37: 1197-1204
[21] Bao S-D (鲍士旦). Soil and Agricultural Chemical Analysis. Beijing: China Agriculture Press, 2000 (in Chinese)
[22] Spohn M, Kuzyakov Y. Phosphorus mineralization can be driven by microbial need for carbon. Soil Biology and Biochemistry, 2013, 61: 69-75
[23] Fisk M, Santangelo S, Minick K. Carbon mineralization is promoted by phosphorus and reduced by nitrogen addition in the organic horizon of northern hardwood forests. Soil Biology and Biochemistry, 2015, 81: 212-218
[24] Tan H, Barret M, Mooij MJ, et al. Long-term phosphorus fertilisation increased the diversity of the total bacterial community and the phod phosphorus mineraliser group in pasture soils. Biology and Fertility of Soils, 2013, 49: 661-672
[25] Li Z-Q (李增强), Zhao B-Z (赵炳梓), Zhang J-B (张佳宝). Application of ¹³C-labeled PLFA analysis in soil microbial ecology studies. Chinese Journal of Eco-Agriculture (中国生态农业学报), 2016, 24(4): 470-477 (in Chinese)
[26] Zhang J-C (张坚超), Xu Y-Q (徐镱钦), Lu Y-H (陆雅海). Microbial mechanisms of methane production and oxidation in terrestrial ecosystems. Acta Ecologica Sinica (生态学报), 2015, 35(20): 6592-6603 (in Chinese)
[27] Tang Z-Z (汤珍珠), Zhu Z-K (祝贞科), Shen B-J (沈冰洁), et al. Effect of stoichiometric ratio of soil nutrients on mineralization and priming effect of glucose in paddy soil. Acta Pedologica Sinica (土壤学报), 2017, 54(1): 246-254 (in Chinese)
[28] Nemergut DR, Townsend AR, Sattin SR, et al. The effect of chronic nitrogen fertilization on alpine tundra soil microbial communities: Implication for carbon and nitrogen cycling. Environmental Microbiology, 2010, 10: 3093-3105
[29] Liu JN, Xu HS, Jiang Y, et al. Methane emissions and microbial communities as influenced by dual cropping of Azolla along with early rice. Scientific Reports, 2017, 7: 40635
[30] Han B (韩冰), Su T (苏涛), Li X (李信), et al. Research progresses of methanotrophs and methane monooxygenases. Chinese Journal of Biotechnology (生物工程学报), 2008, 24(9): 1511-1519 (in Chinese)
[31] Zheng Y, Zhang LM, He JZ. Immediate effects of nitrogen, phosphorus, and potassium amendments on the methanotrophic activity and abundance in a Chinesepaddy soil under short-term incubation experiment. Journal of Soils and Sediments, 2013, 13: 189-196
[32] Xue J-Y (薛晶月), Zhang H-X (张洪轩), Quan Q (全权), et al. Effect of land-use type on soil carbon mineralization and its priming effect on red soils in the mid-subtropics of China. Chinese Journal of Applied and Environmental Biology (应用与环境生物学报), 2014, 20(3): 516-522 (in Chinese)
[33] Spohn M, Chodak M. Microbial respiration per unit biomass increases with carbon-to-nutrient ratios in forest soils. Soil Biology and Biochemistry, 2015, 81: 128-133
[34] Wu J-M (吴家梅), Ji X-H (纪雄辉), Huo L-J (霍莲杰), et al. Fraction changes of oxidation organic carbon in paddy soil and its correlation with CH₄ emission fluxes. Acta Ecologica Sinica (生态学报), 2013, 33(15): 4599-4607 (in Chinese)
[35] Tang H (汤宏), Shen J-L (沈健林), Liu J-Y (刘杰云), et al. Effects of rice straw fraction on methane and carbon dioxide emission from rice paddy soil. Ecology and Environmental Sciences (生态环境学报), 2016, 25(7): 1125-1133 (in Chinese)
[36] Li X (李霞). Soil Phosphorus Eco-stoichiometric Behavior Integrated with Carbon and Nitrogen Sequestration in Paddy Field: A Mesocosm Investigation. PhD Thesis. Hangzhou: Zhejiang University, 2014 (in Chinese)
[37] Liao C (廖畅), Tian Q-X (田秋香), Wang D-Y (汪东亚), et al. Effects of labile carbon addition on organic carbon mineralization and microbial growth stra-tegies in subtropical forest soils. Chinese Journal of Applied Ecology (应用生态学报), 2016, 27(9): 2848-2854 (in Chinese)

稻田土壤有机碳矿化及其激发效应对磷添加的响应

Responses of organic carbon mineralization and priming effect to phosphorus addition in paddy soils.

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	刘珊珊, 王全成, 史加勉, 刘子恺, 沈菊培, 贺纪正, 郑勇. 亚热带森林菌根植物根系真菌群落结构对氮磷添加的响应 [J]. 应用生态学报, 2023, 34(6): 1547-1554.
[2]	豆梦珂, 张伟东, 杨庆朋, 陈龙池, 刘晔嘉, 胡亚林. 杉木种植和磷添加对土壤微生物生物量及胞外酶活性的影响 [J]. 应用生态学报, 2023, 34(3): 631-638.
[3]	黄雪梅, 陈龙池, 田宁, 关欣, 胡亚林, 黄苛, 宿秀江, 陶晓. 松毛虫虫食叶和排泄物对土壤激发效应的影响 [J]. 应用生态学报, 2023, 34(3): 770-776.
[4]	陈甜, 元方慧, 张琳梅, 胡亚林. 不同化学性质叶凋落物添加对土壤有机碳矿化及激发效应的影响 [J]. 应用生态学报, 2022, 33(10): 2602-2610.
[5]	徐敏, 刘苑苑, 元晓春, 曾泉鑫, 林惠瑛, 吴晓霞, 崔琚琰, 陈文伟, 陈岳民. 氮沉降下不同碳添加模式对亚热带毛竹林土壤激发效应的影响 [J]. 应用生态学报, 2022, 33(10): 2619-2627.
[6]	田茂辉, 沈李东, 刘心, 杨王挺, 金靖昊, 杨钰铃, 刘佳琦. 稻田土壤亚硝酸盐型甲烷厌氧氧化菌群落结构的时空特征 [J]. 应用生态学报, 2022, 33(1): 239-247.
[7]	李佳玉, 吕茂奎, 李晓杰, 姜永孟, 谢锦升. 水分对武夷山草甸土壤有机碳激发效应的影响 [J]. 应用生态学报, 2021, 32(4): 1250-1258.
[8]	刘斌, 陈维, 陈伏生, 唐润钰, 王小东, 程怡晴, 卜文圣. 九连山次生阔叶林幼苗生长对氮磷添加的响应 [J]. 应用生态学报, 2020, 31(8): 2533-2540.
[9]	魏夏新, 熊俊芬, 李涛, 文炯, 曾希柏, 余德海. 有机物料还田对双季稻田土壤有机碳及其活性组分的影响 [J]. 应用生态学报, 2020, 31(7): 2373-2380.
[10]	杨静怡, 王旭, 孙立飞, 王超, 白娥. 氮磷添加对长白山温带森林土壤微生物群落组成和氨基糖的影响 [J]. 应用生态学报, 2020, 31(6): 1948-1956.
[11]	史登林, 王小利, 段建军, 刘安凯, 罗安焕, 李瑞东, 侯再芬. 氮肥减量配施生物炭对黄壤稻田土壤有机碳活性组分和矿化的影响 [J]. 应用生态学报, 2020, 31(12): 4117-4124.
[12]	胡伟, 向建华, 向言词, 陈燕. 氮掺杂碳纳米颗粒对稻田根际土壤细菌群落的影响 [J]. 应用生态学报, 2020, 31(11): 3842-3850.
[13]	黄双双, 霍常富, 解宏图, 王朋, 程维信. 表层和下层免耕黑土有机碳矿化速率及激发效应 [J]. 应用生态学报, 2019, 30(6): 1877-1884.
[14]	曹乾斌, 王邵军, 任玉连, 张哲, 陈闽昆, 李少辉, 曹润, 王平, 左倩倩. 蚂蚁筑巢对西双版纳热带森林土壤碳矿化动态的影响 [J]. 应用生态学报, 2019, 30(12): 4231-4239.
[15]	杨全, 陈志飞, 周俊杰, 赖帅彬, 简春霞, 王智, 徐炳成. 黄土丘陵区草地植被群落优势种叶片功能性状对氮磷添加的响应 [J]. 应用生态学报, 2019, 30(11): 3697-3706.