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应用生态学报 ›› 2016, Vol. 27 ›› Issue (8): 2636-2644.doi: 10.13287/j.1001-9332.201608.013

• 目次 • 上一篇    下一篇

黄顶菊入侵对土壤中主要功能细菌的影响

宋振1, 纪巧凤1, 付卫东1, 张瑞海1, 张婷1, 晏静1,2, 张国良1*   

  1. 1中国农业科学院农业环境与可持续发展研究所, 北京 100081;
    2山东农业大学植物保护学院, 山东泰安 271018
  • 收稿日期:2015-12-30 发布日期:2016-08-18
  • 通讯作者: * E-mail: zhangguoliang@caas.cn
  • 作者简介:宋振,男,1985年生,博士,助理研究员.主要从事环境微生物学研究.E-mail:songzhen@caas.cn
  • 基金资助:
    本文由公益性行业(农业)科研专项(201103027)和国家自然科学基金项目(41501280)资助

Effects of Flaveria bidentis invasion on the diversity of functional bacteria in rhizosphere soil.

SONG Zhen1, JI Qiao-feng1, FU Wei-dong1, ZHANG Rui-hai1, ZHANG Ting1, YAN Jing1,2, ZHANG Guo-liang1*   

  1. 1Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    2College of Plant Protection, Shandong Agricultural University, Tai’an 271018, Shandong, China
  • Received:2015-12-30 Published:2016-08-18
  • Contact: * E-mail: zhangguoliang@caas.cn
  • Supported by:
    This work was supported by Special Fund for Agro-scientific Research in the Public Interest (201103027) and Natio-nal Natural Science Foundation of China (41501280)

摘要: 以我国北方大面积发生的入侵植物黄顶菊为研究对象,对黄顶菊根际土壤中可培养的主要功能细菌进行了分离筛选,通过rep-PCR聚类和多样性分析研究了其群落结构的变化,并利用16S rRNA序列比对,对主要优势菌群进行鉴定.结果表明: 相对于本地植物万寿菊和空白对照,黄顶菊显著增加了根际土壤中固氮菌、有机磷细菌、无机磷细菌和钾细菌的数量.rep-PCR分析显示,黄顶菊根际4种功能细菌的种群结构与本地植物和对照相比有显著差异,3种土壤中相同的聚类群极少.多样性分析表明,黄顶菊根际微生物物种多样性更加丰富,群落结构更加复杂,优势种群比较明显,生态多样性较高.对从3种土壤中分离得到的主要优势菌群的鉴定结果也进一步证明了这一结论.研究结果为阐明黄顶菊入侵的微生态机制提供了理论基础.

Abstract: Flaveria bidentis is an invasive plant found in large areas of northern China. This study conducted a separation and screening of the main functional bacteria in the soil of F. bidentis and investigated the change in the community structure. A variety of functional microbes were isolated using selective media and rep-PCR clustering, and a diversity analysis was carried out. In addition, the dominant populations of various functional bacteria were identified using 16S rRNA sequence alignment. The results showed that F. bidentis increased the contents of the major available nutrients in the soil, and the levels of azotobacteria, organic phosphorus bacteria, inorganic phosphorus bacteria, and silicate bacteria in the soil of F. bidentis were significantly higher than those of the native plant Tagetes erecta and the control. rep-PCR analysis indicated that the structure of the four functional bacterial microfloras in the soil of F. bidentis was significantly different from those of the native plant and control. The diversity analysis demonstrated that the diversity of functional microorganisms in the soil of F. bidentis was richer, the community structure was more complex, the predominant microflora comprised a greater proportion of the total population, and the ecological diversity was higher. This was further evidenced by identification of the main functional isolates from the three soil samples. Our findings indicated a mechanism of invasion by F. bidentis.