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应用生态学报 ›› 2016, Vol. 27 ›› Issue (10): 3361-3370.doi: 10.13287/j.1001-9332.201610.034

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余姚滨海不同盐碱度土壤微生物群落组成及土壤酶活性的变化

孙慧1, 张建锋1*, 许华森1, 陈光才1, 王利平2   

  1. 1中国林业科学研究院亚热带林业研究所, 浙江富阳 311400;
    2浙江省余姚市林业特产技术推广总站, 浙江余姚 315400;
  • 收稿日期:2016-03-03 发布日期:2016-10-18
  • 通讯作者: * E-mail: zhangk126@126.com
  • 作者简介:孙 慧, 女, 1990年生, 硕士. 主要从事退化土地生态修复研究. E-mail: kikotilamisu@163.com
  • 基金资助:
    本文由国家林业局“948”项目(2013-4-83)资助

Variations of soil microbial community composition and enzyme activities with different salinities on Yuyao coast, Zhejiang, China

SUN Hui1, ZHANG Jian-feng1*, XU Hua-sen1, CHEN Guang-cai1, WANG Li-ping2   

  1. 1Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China;
    2Yuyao Forestry Technological Extension Station, Yuyao 315400, Zhejiang, China;
  • Received:2016-03-03 Published:2016-10-18
  • Contact: * E-mail: zhangk126@126.com
  • Supported by:
    This work was supported by the ‘948’ Project (2013-4-83) from State Forestry Administration of China.

摘要: 在2015年10月于余姚滨海采集不同盐碱度的土壤,对盐碱地土壤微生物群落组成、土壤过氧化氢酶、脲酶活性及土壤理化性质的变化规律进行了研究.结果表明:硝化螺菌属在细菌群落中占绝对优势,其与全钾最相关;枝孢属和镰刀菌属在真菌群落中占主要优势,脲酶、全氮与枝孢属的相关性最大,同时脲酶与镰刀菌属也最相关.各盐碱地表层土细菌和真菌群落的物种组成最丰富且均匀程度高.重度、中度及轻度盐碱地土壤过氧化氢酶活性分别为3.52~4.56、3.08~4.61及5.81~6.91 mL·g-1,过氧化氢酶活性均表现为随土层加深而逐渐增大的特征;土壤全钾和过氧化氢酶直接相关,pH、有机质、全氮和全磷通过全钾与过氧化氢酶间接相关.各盐碱度土壤脲酶活性分别为0.04~0.52、0.08~1.07及0.27~8.21 mg·g-1,脲酶活性均呈现为随土层加深而逐渐减小的趋势;土壤全氮和脲酶直接相关,pH、有机质和全钾通过全氮与脲酶间接相关.CCA排序表明,全磷对细菌群落的影响程度最大,而脲酶对真菌群落的影响最大.

关键词: 土壤酶, 盐碱土, 植被构建, 通径分析, 土壤微生物, 典范对应分析

Abstract: In October 2015, soil samples with different salinity were collected in a coast area in Yuyao, Zhejiang, and soil microbial community composition, soil catalase, urease activities, as well as soil physical and chemical properties were studied. The results showed that Nitrospira took absolute advantage in the bacterial community, and showed good correlations to total potassium. Cladosporium and Fusarium were predominant in the fungal community. Meanwhile, Cladosporium was related to soil urease and total nitrogen, and same correlation was found between Fusarium and soil urease. Catalase activity ranged from 3.52 to 4.56 mL·g-1, 3.08 to 4.61 mL·g-1 and 5.81 to 6.91 mL·g-1 for soils with heavy, medium and weak salinity, respectively. Catalase activity increased with the soil layer deepening, which was directly related to soil total potassium, and indirectly related to pH, organic matter, total nitrogen and total phosphorus through total potassium. Soil urease activity ranged among 0.04 to 0.52 mg·g-1, 0.08 to 1.07 mg·g-1 and 0.27 to 8.21 mg·g-1 for each saline soil, respectively. Urease activity decreased with soil layer deepening which was directly related to soil total nitrogen, and was indirectly related to pH, organic matter and total potassium through total nitrogen. The total phosphorus was the largest effect factor on the bacterial community CCA ordination, and the urease was on fungal community.

Key words: soil microorganism, soil enzyme, saline soil, vegetation restoration, canonical correspondence analysis, path analysis