Abstract: Addition of urease inhibitors is one of the important measures to increase nitrogen (N) use efficiency of crop, due to retardant of urea hydrolysis and reduction of ammonia volatilization loss. An incubation experiment was conducted to investigate the urease inhibition effect of a new phosphoramide urease inhibitor, NPPT (N-(n-propyl) thiophosphoric triamide) in different texture soils under dark condition at 25 ℃, and NBPT (N-(n-butyl) thiophosphoric triamide) was obtained to compare the inhibition effect on urease in different soil textures by different dosages of urea adding. Results showed that the effective reaction time of urea was less than 9 d in the loamy and clay soil. Addition of inhibitors for retardation of urea hydrolysis was more than 3 d. In sandy soil, urea decomposition was relatively slow, and adding inhibitor significantly inhibited soil urease acti-vity, and reduced NH₄⁺-N content. During the incubation time, the inhibition effect of high dosage urea in the soil was better than that of low dosage. At day 6, the urease inhibition rate of NBPT and NPPT (N 250 mg·kg^-1) were 56.3% and 53.0% in sandy soil, 0.04% and 0.3% in loamy soil, 4.1% and 6.2% in clay soil; the urease inhibition rate of NBPT and NPPT (N 500 mg·kg^-1) were 59.4% and 65.8% in sandy soil, 14.5% and 15.1% in loamy soil, 49.1% and 48.1% in clay soil. The urease inhibition effects in different texture soil were in order of sandy soil > clay soil> loamy soil. The soil NH₄⁺-N content by different inhibitors during incubation time increased at first and then decreased, while soil NO₃^--N content and apparent nitrification rate both showed rising trends. Compared with urea treatment, addition of urease inhibitors (NBPT and NPPT) significantly increased urea-N left in the soil and reduced NH₄⁺-N content. In short, new urease inhibitor NPPT in different texture is an effective urease inhibitor.