配施磷细菌肥对复垦土壤细菌多样性及磷有效性的影响

doi:10.13287/j.1001-9332.201609.040

应用生态学报 ›› 2016, Vol. 27 ›› Issue (9): 3016-3022.doi: 10.13287/j.1001-9332.201609.040

配施磷细菌肥对复垦土壤细菌多样性及磷有效性的影响

孟会生¹, 洪坚平^1*, 杨毅¹, 王向英², 李廷亮¹, 栗丽¹

1山西农业大学资源环境学院, 山西太谷 030801;
2山西农业大学生命科学学院, 山西太谷 030801

收稿日期:2016-03-11 发布日期:2016-09-18
通讯作者: * E-mail: hongjpsx@163.com
作者简介:孟会生,男,1977年生,博士研究生. 主要从事土壤复垦与生态重建研究. E-mail: huishengmeng@126.com
基金资助:
国家自然科学基金项目(31272257,4140011839)和国家公益性行业专项(201103004-5)资助

Effect of applying phosphorus bacteria fertilizer on bacterial diversity and phosphorus availability in reclaimed soil.

MENG Hui-sheng¹, HONG Jian-ping^1*, YANG Yi¹, WANG Xiang-ying², LI Ting-liang¹, LI Li¹

1College of Resources and Environmental Science, Shanxi Agricultural University, Taigu 030801,Shanxi, China;
2College of Life Science, Shanxi Agricultural University, Taigu 030801, China

Received:2016-03-11 Published:2016-09-18
Contact: * E-mail: hongjpsx@163.com
Supported by:
This work was supported by the National Natural Science Foundation of China (31272257, 4140011839) and the special fund for the Scientific Research in the Public Interest of China (201103004-5)

摘要/Abstract

摘要： 为了解磷细菌肥对复垦土壤微生物群落结构变化特征和磷有效性的影响,本研究以连续配施磷细菌肥5年的定位试验为背景,利用16S rDNA基因序列测序方法对土壤细菌群落多样性进行分析,探讨土壤细菌群落与土壤Olsen-P、碱性磷酸酶的关系.试验共设对照、单施化肥、有机肥、有机肥化肥、化肥磷细菌肥、有机肥磷细菌肥和有机肥化肥磷细菌肥7个处理.结果表明: 复垦土壤中放线菌门和变形菌门菌群的相对丰度最大,分别为21.6％～32.2％和13.8％～28.9％.有机肥化肥磷细菌肥处理的OTU数和Chao1指数分别为809和26190,均属最高.磷细菌肥处理能提高土壤中放线菌门和变形菌门菌群的相对丰度,降低土壤中酸杆菌门、热袍菌门和硝化螺旋菌门菌群的相对丰度,对诺卡氏菌属、屈挠杆菌属有一定的促进作用.有机肥化肥磷细菌肥处理能够提高复垦土壤Olsen-P及碱性磷酸酶活性.复垦土壤变形菌门与Olsen-P、碱性磷酸酶的相关系数最高(0.900、0.955),在一定程度上可以作为土壤磷有效性的灵敏性指标.

Abstract: A located field experiment of applying phosphorus bacteria fertilizer for five years was carried out to study the effects of applying phosphorus bacteria fertilizer on the characteristics of microbial community structure in reclaimed soil. We studied the diversity of bacterial community using 16S rDNA gene sequencing and analyzed the relationship between bacterial community and Olsen-P, alkaline phosphatase. Seven treatments including control, chemical fertilizer, manure, manure and chemical fertilizer, chemical fertilizer and phosphorus bacteria, manure and phosphorus bacteria, and, manure,chemical fertilizer and phosphorus bacteria were conducted. The results showed that the relative abundance of Actinobacteria and Proteobacteria in reclaimed soil was the largest, which was 21.6％-32.2％ and 13.8％-28.9％, respectively. Operational taxonomic units (OTU) number and Chao1 index of the treatment of manure, chemical fertilizer and phosphorus bacteria fertilizer was 809 and 26190, which was the highest. Phosphorus bacteria fertilizer could improve the relative abundance of soil Actinomycetes and Proteobacteria and decrease that of soil Acidobacteria, Thermotogae and Nitrospira, and had stimulatory effect on Nocardioides and Flexibacter. The treatment of manure, chemical fertilizer and phosphorus bacteria fertilizer could improve the Olsen-P and alkaline phosphatase activities in reclaimed soil. Correlation coefficients between Proteobacteria and Olsen-P, alkaline phosphatase were the highest (0.900 and 0.955). To a certain extent, Proteobacteria could be used as the sensitivity index of soil phosphorus availability.

孟会生, 洪坚平, 杨毅, 王向英, 李廷亮, 栗丽. 配施磷细菌肥对复垦土壤细菌多样性及磷有效性的影响[J]. 应用生态学报, 2016, 27(9): 3016-3022.

MENG Hui-sheng, HONG Jian-ping, YANG Yi, WANG Xiang-ying, LI Ting-liang, LI Li. Effect of applying phosphorus bacteria fertilizer on bacterial diversity and phosphorus availability in reclaimed soil.[J]. Chinese Journal of Applied Ecology, 2016, 27(9): 3016-3022.

参考文献

[1] Liu F (刘飞), Lu L (陆林). Progress on the study of ecological restoration of coal mining subsidence areas. Journal of Natural Resources (自然资源学报), 2009, 24(4): 612-620 (in Chinese)
[2] Li J-H (李建华), Gao C-H (郜春花), Lu C-D (卢朝东), et al. Ecological effects of arbuscular mycorrhiza and rhizobium on mining land reclamation. Soil and Fertilizer Sciences in China (中国土壤与肥料), 2009(5): 77-80 (in Chinese)
[3] Thomas B, Newman JA, Silverman BW, et al. The contribution of species richness and composition to bacterial services. Nature, 2005, 436: 1157-1160
[4] Griffiths BS, Ritz K, Bardgett RD, et al. Ecosystem response of pasture soil communities to fumigation-induced microbial diversity reductions: An examination of the biodiversity-ecosystem function relationship. Oikos, 2000, 90: 279-294
[5] Dangi SR, Stahl PD, Wick AF, et al. Soil microbial community recovery in reclaimed soils on a surface coal mine site. Soil Science Society of America Journal, 2012, 76: 915-924
[6] Guo C-J (郭春景), Guan Z-H (关兆红), Li Y-W (李玉文), et al. Effects of bio-organic fertilizer on the micro-ecology of ginseng-culturing fields. Biotechnology (生物技术), 2004, 14(3): 55-56 (in Chinese)
[7] Li L (栗丽), Hong J-P (洪坚平), Xie Y-H (谢英荷), et al. Effect of bacterial manure on soil biological activity, yield and quality of rape in reclaimed core-mining area. Chinese Journal of Eco-Agriculture (中国生态农业学报), 2010, 18(5): 939-944 (in Chinese)
[8] Sun Z-T (孙中涛), Yao L-T (姚良同), Sun F-M (孙凤鸣), et al. Effects of microbial fertilizer on the soil ecosystem and growth of cotton. Chinese Journal of Eco-Agriculture (中国生态农业学报), 2005, 13(3): 54-56 (in Chinese)
[9] Li J-L (李金岚), Hong J-P (洪坚平), Xie Y-H (谢英荷), et al. Effects of different fertilization treatments on reclaimed soil microbial community structure in core-mining subsidence area. Acta Ecologica Sinica (生态学报), 2010, 30(22): 6193-6200 (in Chinese)
[10] Zhong S-T (钟书堂), Shen Z-Z (沈宗专), Sun Y-F (孙逸飞), et al. Effects of continuous application of bio-organic fertilizer on banana production and cultural microflora of bulk soil in orchard with serious disease incidence. Chinese Journal of Applied Ecology (应用生态学报), 2004, 15(2): 237-240 (in Chinese)
[11] Niu X (牛旭). Effects of Bio-organic Fertilizer on Reclaimed Soil Microbial Diversity and Wheat Growth. Master’s Thesis. Taiyuan: Shanxi University, 2014 (in Chinese)
[12] Lou J (楼骏), Liu Y (柳勇), Li Y (李延). Review of high-throughput sequencing techniques in studies of soil microbial diversity. Chinese Agricultural Science Bulletin (中国农学通报), 2014, 30(15): 256-260 (in Chinese)
[13] Chu H-Y (褚海燕), Fierer N, Christian LL, et al. Soil bacterial diversity in the Arctic is not fundamentally different from that found in other biomes. Environmental Microbiology, 2010, 12: 2998-3006
[14] Bao S-D (鲍士旦). Methods of Soil and Agricultural Chemistry Analysis. 3rd Ed. Beijing: Chinese Agriculture Press, 2008
[15] Malik MA, Marschner P, Khan KS. Addition of organic and inorganic P sources to soil-effects on P pools and microorganisms. Soil Biology and Biochemistry, 2012, 49: 106-113
[16] Zou Y-D (邹原东), Fan J-H (范继红). Review on effect of organic fertilizer on soil fertility. Chinese Agricultural Science Bulletin (中国农学通报), 2013, 29(3): 12-16 (in Chinese)
[17] Quadros PDD, Zhalnina K, Davis-Richardson AG, et al. Coal mining practices reduce the microbial biomass, richness and diversity of soil. Applied Soil Ecology, 2016, 98: 195-203
[18] Li Y-Y (李媛媛), Zhang Y-K (张怡康). Bacteria community diversity coal mining subsidence in Xuzhou land reclamation soil. Jiangsu Agricultural Sciences (江苏农业科学), 2014, 42(9): 312-315 (in Chinese)
[19] Long J (龙健), Huang C-Y (黄昌勇), Teng Y (滕应), et al. Microbial eco-characterization and its restoration in copper reclaimed wasteland in red soil area of China. Ⅱ. Effects on soil microbial characteristics and community structure. Chinese Journal of Applied Ecology (应用生态学报), 2015, 26(2): 481-489 (in Chinese)
[20] Sun J-J (孙家骏), Fu Q-X (付青霞), Gu J (谷洁), et al. Effects of bio-organic fertilizer on soil enzyme activities and microbial community in kiwifruit orchard. Chinese Journal Applied Ecology (应用生态学报), 2016, 27(3): 829-837 (in Chinese)
[21] Chen L (陈琳), Gu J (谷洁), Hu T (胡婷), et al. Effects of biological organic on microbial community’s metabolic activity in a soil planted with chestnut. Chinese Journal of Applied Ecology (应用生态学报), 2013, 24(6): 1627-1632 (in Chinese)
[22] Yang Y-L (杨玉莲), Wu F-Z (吴福忠), He Z-H (何振华), et al. Effects of snow pack removal on soil microbial biomass carbon and nitrogen and the number of soil culturable microorganisms during wintertime in alpine Abies faxoniana forest of western Sichuan, Southwest China. Chinese Journal of Applied Ecology (应用生态学报), 2012, 23(7): 1809-1816 (in Chinese)
[23] Ma X-X (马晓霞), Wang L-L (王莲莲), Li Q-H (黎青慧), et al. Effects of long-term fertilization on soil microbial biomass carbon and nitrogen and enzyme acti-vities during maize growing season. Acta Ecologica Sinica (生态学报), 2012, 32(17): 5502-5511 (in Chinese)
[24] Sessitsch A, Weilharter A, Gerzabek MH, et al. Microbial population structures in soil particle size fractions of a long-term fertilizer field experiment. Applied and Environmental Microbiology, 2001, 67: 4215-4224
[25] Hansel CM, Fendorf S, Jardine PM, et al. Changes in bacterial and archaeal community structure and functional diversity along a geochemically variable soil profile. Applied and Environmental Microbiology, 2008, 74: 1620-1633
[26] Grégoire P, Fardeau ML, Joseph M, et al. Isolation and characterization of Thermanaerothrix daxensis gen. nov., sp. nov., a thermophilic anaerobic bacterium pertaining to the phylum “Chloroflexi”, isolated from a deep hot aquifer in the Aquitaine Basin. Systematic and Applied Microbiology, 2011, 34: 494-497
[27] Costello EK, Schmidt SK. Microbial diversity in alpine tundra wet meadow soil: Novel Chloroflexi from a cold, water-saturated environment. Environmental Microbiology, 2006, 8: 1471-1486
[28] Rashid MI, Mujawar LH, Shahzad T, et al. Bacteria and fungi can contribute to nutrients bioavailability and aggregate formation in degraded soils. Microbiological Research, 2016, 183: 26-41
[29] Kanchikerimath M, Singh D. Soil organic matter and bio-logical properties after 26 years of maize-wheat -cowpea cropping as affected by manure and fertilization in a Cambisol in semiarid region of India. Agriculture, Ecosystems and Environment, 2001, 86: 155-162

配施磷细菌肥对复垦土壤细菌多样性及磷有效性的影响

Effect of applying phosphorus bacteria fertilizer on bacterial diversity and phosphorus availability in reclaimed soil.

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价