土壤盐分对Bt棉铃壳中Bt蛋白含量和氮代谢生理的影响

doi:10.13287/j.1001-9332.201808.030

应用生态学报 ›› 2018, Vol. 29 ›› Issue (8): 2583-2589.doi: 10.13287/j.1001-9332.201808.030

土壤盐分对Bt棉铃壳中Bt蛋白含量和氮代谢生理的影响

王永慧^1,2, 陈建平¹, 孙艳茹¹, 张祥², 陈德华^2*

¹江苏沿海地区农业科学研究所/农业部沿海盐碱地农业科学观测实验站, 江苏盐城 224401;
²扬州大学江苏省作物遗传生理重点实验室, 江苏扬州 225009

收稿日期:2017-11-19 出版日期:2018-08-20 发布日期:2018-08-20
通讯作者: E-mail: cdh@yzu.edu.cn
作者简介:王永慧, 男, 1983年生, 博士, 副研究员. 主要从事作物栽培及生理生态研究. E-mail: huiyw2008@163.com
基金资助:
本文由国家自然科学基金项目(31501267)、国家重点研发计划项目 (2016YFD0101421)、中国农业科学院科技创新工程项目(SYSFHT)、江苏省三新工程项目[SXGC(2016)320]和江苏省农业科技自主创新资金[CX(14)2065]资助

Effects of soil salinity on Bt protein content and nitrogen metabolic physiology in boll shell of Bt cotton

WANG Yong-hui^1,2, CHEN Jian-ping¹, SUN Yan-ru¹, ZHANG Xiang², CHEN De-hua^2*

¹Institute of Agricultural Sciences of Jiangsu Coastal Area/Observation and Experimental Station of Saline Land of Costal Area, Ministry of Agriculture, Yancheng 224401, Jiangsu, China;
²Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou 225009, Jiangsu, China.

Received:2017-11-19 Online:2018-08-20 Published:2018-08-20
Supported by:
This work was supported by the National Natural Science Foundation of China (31501267), the National Key Research and Development Support Program of China (2016YFD0101421), the Technology Innovation Project of Chinese Academy of Agricultural Sciences (SYSFHT), the Jiangsu “Three-innovation” Agricultural Project [SXGC(2016)320], and the Jiangsu Agricultural Science and Technology Innovation Fund [CX(14)2065].

摘要/Abstract

摘要： 为探讨土壤盐分对转Bt基因抗虫棉棉铃抗虫性的影响,以2个Bt棉品种‘新棉33B’(盐敏感)和‘中07’(耐盐)为试验材料,设置5个土壤盐分水平(0、0.15%、0.30%、0.45%和0.60%),采用盆栽法研究了不同土壤盐分下Bt棉盛铃期铃壳Bt蛋白含量变化及氮代谢生理特征.结果表明: 铃壳中Bt蛋白含量随土壤盐分的升高而降低,与对照相比(0%),盐敏感型品种在土壤盐分0.15%、耐盐型品种在0.30%以上时,铃壳中Bt蛋白含量显著下降.相同土壤盐分水平下,花后30 d(DPA)铃壳Bt蛋白含量下降幅度明显大于10 DPA.在铃壳Bt杀虫蛋白含量下降显著时,其可溶性蛋白含量明显下降,硝酸还原酶(NR)和谷氨酸丙酮酸转氨酶(GPT)活性显著下降;游离氨基酸含量、蛋白酶和肽酶活性在土壤盐分0.30%以上时显著增加.以上结果说明,土壤盐分影响铃壳的氮代谢,从而引起Bt蛋白合成减弱,中度以上土壤盐分水平会引起Bt蛋白分解加强,从而导致杀虫蛋白表达水平进一步下降.

Abstract: In order to clarify the effects of soil salinity on the insect-resistance of boll in transgenic Bt cotton, potted plants of two Bt cotton cultivars Xinmian 33B (salt-sensitive) and Zhong 07 (salt-tolerant) were exposed to five levels of soil salinity (0, 0.15%, 0.30%, 0.45% and 0.60%). The results showed that Bt protein content of boll shell decreased with increasing soil salinity. Compared with the control (0% soil salinity level), the Bt protein content of boll shell decreased significantly when the soil salinity level was above 0.15% for Xinmian 33B and above 0.30% for Zhong 07. The reduction extent of Bt protein content of boll shell at 30 days post anthesis (DPA) was greater than that at 10 DPA under the same soil salinity level. Significant reductions of soluble protein contents, nitrate reductase (NR), and glutamate pyruvate transaminase (GPT) activities were observed when the boll shell Bt protein content was significantly reduced. The content of free amino acid, protease, and peptidase activity of boll shell significantly increased when the soil salinity level was above 0.30%. In conclusion, soil salinity affected boll shell nitrogen metabolism and reduced Bt protein synthesis. Middle and high soil salinity levels could enhance decomposition of Bt protein, which further decreases the expression level of insecticidal protein.

王永慧, 陈建平, 孙艳茹, 张祥, 陈德华. 土壤盐分对Bt棉铃壳中Bt蛋白含量和氮代谢生理的影响[J]. 应用生态学报, 2018, 29(8): 2583-2589.

WANG Yong-hui, CHEN Jian-ping, SUN Yan-ru, ZHANG Xiang, CHEN De-hua. Effects of soil salinity on Bt protein content and nitrogen metabolic physiology in boll shell of Bt cotton[J]. Chinese Journal of Applied Ecology, 2018, 29(8): 2583-2589.

参考文献

[1] Clive J. Global biotechnology/GM crops commercialization development trend in 2015. China Biotechnology (中国生物工程杂志), 2016, 36(4): 1-11 (in Chinese)
[2] Huang J-K (黄季焜), Mi J-W (米建伟), Lin H (林海) , et al. A decade of Bt cotton in farmer fields in China: Assessing the direct effects and indirect externa-lities of Bt cotton adoption in China. Scientia Sinica Vitae (中国科学: 生命科学), 2010, 40(3): 260-272 (in Chinese)
[3] Kumar R, Dahiya KK, Kumar D. Evaluation of Bt cotton hybrids against bollworms in cotton. Annals of Agri Bio Research, 2013, 18: 39-43
[4] Wang D-M (王冬梅), Li H-Q (李海强), Ding R-F (丁瑞丰), et al. Spatio-temporal expression of foreign Bt insecticidal protein in transgenic Bt cotton varieties in northern Xinjiang, China. Cotton Science (棉花学报), 2012, 24(1): 18-26 (in Chinese)
[5] Olsena KM, Dalya JC, Holta HE, et al. Season-long variation in expression of Cry1Ac gene and efficacy of Bacillus thuringiensis toxin in transgenic cotton against Helicoverpa armigera (Lepidoptera: Noctuidae). Journal of Economic Entomology, 2005, 98: 1007-1017
[6] Downes S, Mahon R. Evolution, ecology and management of resistance in Helicoverpa spp. to Bt cotton in Australia. Journal of Invertebrate Pathology, 2012, 110: 281-286
[7] Manjunatha R, Pradeep S, Sridhara S, et al. Quantification of Cry1Ac protein over time in different tissues of Bt cotton hybrids. Karnataka Journal of Agricultural Sciences, 2009, 22: 609-610
[8] Wan P, Zhang YJ, Wu KM, et al. Seasonal expression profiles of insecticidal protein and control efficacy against Helicoverpa armigera for Bt cotton in the Yangtze River valley of China. Journal of Economic Entomology, 2005, 98: 195-201
[9] Chen WP, Hou ZA, Wu LS, et al. Effects of salinity and nitrogen on cotton growth in arid environment. Plant and Soil, 2010, 326: 61-73
[10] Jiang LJ, Duan LS, Tian XL, et al. NaCl salinity stress decreased Bacillus thuringiensis (Bt) protein content of transgenic Bt cotton (Gossypium hirsutum L.) seedlings. Environmental and Experimental Botany, 2006, 55: 315-320
[11] Luo Z, Dong HZ, Li WJ, et al. Individual and combined effects of salinity and waterlogging on Cry1Ac expression and insecticidal efficacy of Bt cotton. Crop Protection, 2008, 27: 1485-1490
[12] Xin C-S (辛承松), Dong H-Z (董合忠), Wen S-M (温四民), et al. Salt tolerance appraisal of Bt cotton with different genotypes in coastal saline soil. Chinese Agricultural Science Bulletin (中国农学通报), 2008, 24(2): 188-192 (in Chinese)
[13] Zhang L-N (张丽娜), Ye W-W (叶武威), Wang J-J (王俊娟), et al. Genetic diversity analysis of salinity related germplasm in cotton. Biodiversity Science (生物多样性), 2010, 18(2): 142-149 (in Chinese)
[14] Wang Y-H (王永慧), Chen J-P (陈建平), Cai L-W (蔡立旺), et al. Effect of combination stress of tempe-rature and humidity on insecticidal protein expression of Bt transgenic cotton. Cotton Science (棉花学报), 2013, 25(1): 63-67 (in Chinese)
[15] Zhang X-Z (张宪政). Research Methods of Crops’ Physiology. Beijing: China Agriculture Press, 1992 (in Chinese)
[16] Institute of Shanghai Plant Physiology, Chinese Academy of Sciences (中国科学院上海植物生理研究所). Guide to the Experiment of Modern Plant Physiology. Shanghai: Science Press, 1999 (in Chinese)
[17] Carrasco P, Carbonell J. Changes in the level of peptidase activities in pea ovaries during senescence and fruit set induced by gibberellic acid. Plant Physiology, 1990, 92: 1070-1074
[18] Setlow P. Protease and peptidase activities in growing and sporulating cells and dormant spores of Bacillus megaterium. Journal of Bacteriology, 1975, 122: 642-649
[19] Luo J-Y (雒珺瑜), Cui J-J (崔金杰), Zhang S (张帅), et al. The effects of temporal and spatial variation of exogenous protein of transgenic Bt cotton to insect resistance in saline-alkali soil. Acta Ecologica Sinica (生态学报), 2017, 37(16): 5474-5481 (in Chinese)
[20] Li M-Y (李茂营). Effect of NaCl on Growth and the Insect-resistance Efficiency of Transgenic Bt Cotton (Gossypium hirsutum L.) and Application Plant Regulator Alleviate the Adverse Effects. PhD Thesis. Beijing: China Agricultural University, 2014 (in Chinese)
[21] Benedict JH, Sachs ES, Altman DW, et al. Field performance of cottons expressing transgenic Cry1A insecticidal proteins for resistance to Heliothis virescens and Helicoverpa zea (Lepidoptera: Noctuidae). Journal of Economic Entomology, 1996, 89: 230-238
[22] Dong HZ, Li WJ. Variability of endotoxin expression in Bt transgenic cotton. Journal of Agronomy and Crop Science, 2007, 193: 21-29
[23] Stam M, Mol JNM, Kooter JM. The silence of genes in transgenic plants. Annals of Botany, 1997, 79: 3-12
[24] Xia L-Q (夏兰芹), Guo S-D (郭三堆). The expression of Bt toxin gene under different thermal treatments. Scientia Agricultura Sinica (中国农业科学), 2004, 37(11): 1733-1737 (in Chinese)
[25] Chen Y (陈源), Han Y (韩勇), Wang J (王俊), et al. Effects of high temperature on Bt proteins expression and nitrogen metabolic physiology in square of Bt cotton at the peak squaring stage. Chinese Journal of Applied Ecology (应用生态学报), 2014, 25(9): 2623-2628 (in Chinese)
[26] Chen DH, Ye GY, Yang CQ, et al. The effect of high temperature on the insecticidal properties of Bt cotton. Environmental and Experimental Botany, 2005, 53: 333-342
[27] Luo Z, Dong HZ, Li WJ, et al. Individual and combined effects of salinity and waterlogging on Cry1Ac expression and insecticidal efficacy of Bt cotton. Crop Protection, 2008, 27: 1485-1490
[28] Sadras VO. Compensatory growth in cotton after loss of reproductive organs. Field Crops Research, 1995, 40: 1-18
[29] Mason TG. Growth and abscission in sea island cotton. Annals of Botany, 1922, 36: 457-484
[30] Pettigrew WT, Adamczyk JJ. Nitrogen fertility and planting date effects on lint yield and Cry1Ac (Bt) endotoxin production. Agronomy Journal, 2006, 98: 691-697
[31] Zhang X (张祥), Ma A-L (马爱丽), Fang J (房静), et al. Effect of GA₃ and DPC on Bt protein expression and boll nitrogen metabolism of Bt transgenic cotton. Cotton Science (棉花学报), 2010, 22(2): 150-156 (in Chinese)

土壤盐分对Bt棉铃壳中Bt蛋白含量和氮代谢生理的影响

Effects of soil salinity on Bt protein content and nitrogen metabolic physiology in boll shell of Bt cotton

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价