Abstract: Productivity is low and unstable in dryland farms of central Gansu, Northwest China. Conservational tillage is an important way for the sustainable development of agriculture. The effects of different tillage measures on soil moisture infiltration, evaporation, crop yield and water use efficiency (WUE) were investigated in this study based on a long-term experiment since 2001 in Longzhong. There were six treatments, i.e. conventional tillage with no straw (T), no-till with straw cover (NTS), no-till with no straw cover (NT), conventional tillage with straw incorporated (TS), conventional tillage with plastic mulch (TP), and no-till with plastic mulch (NTP), with annual rotation of spring wheat and pea. The results showed that compared with T, soil bulk density of NTS decreased and total porosity of soil increased significantly in wheat and pea land. Compared with conventional tillage, conservation tillage reduced soil infiltration rate in 0-5 cm in the pea field by 56.2%. Conservational tillage siginificantly increased soil saturated water conductivity in both wheat and pea lands. Compared with T, the saturated water conductivity in NTS was significantly increased by 52.8%-107.1%. Conservational tillage siginificantly reduced soil evaporation during growing season. Compared with T, the evapotranspiration of NTP, TP and NTS was significantly reduced by 14.4%-50.8%. The soil evaporation after rain was also decreased. Conservational tillage improved crop yield and water use efficiency by 9.5%-62.8% and 0.4%-50.9%, respectively. Therefore, conservational tillage could increase water use efficiency and crop yield in dryland farming area of central Gansu, Northwest China.