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常用化学杀螨剂对两种生防真菌孢子的相容性测定

姜渝;冯明光   

  1. 浙江大学应用昆虫学研究所,杭州 310029
  • 收稿日期:2005-08-30 修回日期:2006-02-28 出版日期:2006-07-18 发布日期:2006-07-18

Compatibility of two fungal biocontrol agents conidia with commercial chemical acaricides

JIANG Yu; FENG Mingguang   

  1. Institute of Applied Entomology, Zhejiang University, Hangzhou 310029, China
  • Received:2005-08-30 Revised:2006-02-28 Online:2006-07-18 Published:2006-07-18

摘要: 用营养液萌发法和平板萌发法测定了10种常用化学杀螨剂与球孢白僵菌(Beauveria bassiana)和玫烟色拟青霉(Paecilomyces fumosoroseus)分生孢子的生物相容性.结果表明,营养液萌发法比平板法灵敏.以24 h内的孢子萌发率作为指标,供试杀螨剂的常用浓度及其5和10倍稀释液对活孢率的影响在不同杀螨剂间差异极显著,并在两种真菌间也存在差异.三唑锡、浏阳霉素、三氯杀螨醇和阿维菌素对生防真菌孢子的抑制力很强,不宜混用.速螨酮、克螨特、乐斯本、尼索朗及双甲脒等杀螨剂与真菌孢子的相容性优良,可与菌剂田间混用.将速螨酮、克螨特和乐斯本分别按3种浓度与白僵菌的孢子悬乳剂混配后在4 ℃恒温和室内变温下长期共贮,结果显示,3种杀螨剂对孢子活力的影响迥异,混配制剂在室内变温下的贮存期均未达到12个月,离商品化的要求差距较大.乐斯本的长期相容性相对较优,与菌剂低浓度混配可在常温下贮存6.5个月,中浓度和低浓度混配在4 ℃下可贮存12个月.

关键词: 木质部, 阔叶红松林, 非结构性碳水化合物, 韧皮部, 碳分配, 长白山

Abstract: In this paper, the biological compatibility of fungal biocontrol agents Beauveria bassiana and Paecilomyces fumosoroseus conidia with 10 commercial chemical acaricides were assayed, based on the conidial germination rates in nutritional liquid and on SDAY plate. The results showed that nutritional liquid was more available than SDAY plate in the assay. So far as the 24 h conidial viability concerned, there were significant differences among the test acaricides with the concentrations of recommended for field spray and 5 and 10 fold dilutions, as well as between the two fungal agents. Since acaricides azocyclotin, liuyangmycin, dicofol and avermectin had strong inhibitory effects on the 24 h germination rate of both fungal agents conidia, their combined application with fungal agents was unsuitable for mite control. However, the combined application of pyridaben, propargite, chlorpyrifos, hexythiazox or amitraz with either B. bassiana or P. fumosoroseus was practical, because of their shortterm compatibility. When the mixtures of oil-based B. bassiana formulation with the three concentrations of pyridaben, propargite and chlorpyrifos were stored at 4 ℃ or at ambient temperature for 12 months, none of the three chemicals was considered to be good enough for a combined formulation due to the great variability in long-term compatibility. Nevertheless, chlorpyrifos exhibited an encouraging long-term compatibility with B. bassiana, because its low concentration in the fungal formulation did not affect the conidial viability during a 6.5-month period of storage at ambient temperature. When stored at 4 ℃ in dark, the B. bassiana formulation containing low or medium concentration of chlorpyrifos retained the conidial viability of >90% for up to 12 months.

Key words: phloem, carbon allocation, non-structural carbohydrates, xylem, broadleaved Korean pine forest, Changbai Mountain.