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长白山木贼山柰素含量对反射光谱参数的响应

朱小迪;范秀华;李永慈   

  1. 北京林业大学, 北京 100083
  • 收稿日期:2008-07-04 修回日期:1900-01-01 出版日期:2009-04-10 发布日期:2009-04-10

Responses of kaempferol content in Hippochaete hiemale from Changbai Mountains to spectral reflectance parameters.

ZHU Xiao-di;FAN Xiu-hua;LI Yong-ci   

  1. Beijing Forestry University, Beijing 100083, China
  • Received:2008-07-04 Revised:1900-01-01 Online:2009-04-10 Published:2009-04-10

摘要: 对长白山地区木贼山柰素含量和反射光谱参数的关系进行了研究,探索了判别木贼药材质量优劣的快捷方法。根据反射光谱曲线(Unispec-Sc光谱分析仪)导出的若干参数,对木贼样品进行聚类分析,采用HPLC法分别测定木贼山柰素的含量,分析了反射光谱参数与山柰素含量的相关性。结果表明:蓝边面积(SDb)、绿峰反射率(Rg)等参数与山柰素含量呈显著负相关(r分别为-0.991,-0.982),530~570 nm波段的反射率与山柰素含量亦呈显著的负相关(︱r︱>0.95)。通过比较SDb、Rg,可简捷地判断长白山地区木贼质量的优劣。增强530~570 nm波段的入射光线,可刺激山柰素含量的增加,但需要大田实验进一步验证.

关键词: AM真菌, 抗旱性, 保护系统, 柑橘, 嫁接苗

Abstract: This paper studied the relationships between kaempferol content in Hippochaete hiemale from Changbai Mountains and spectral reflectance parameters, ai med to establish a shortcut method for judging the medicinal quality of this pla nt. According to the spectral reflectance parameters derived from the reflectanc e spectral curves determined by Unispec-Sc spectral analysis system, cluster an alysis was conducted for H. hiemale samples. The correlation analysis between the kaempferol content of this plant determined by HPLC and the spectral reflectanc e parameters showed that the kaempferol content had significant negative correla tions with the area of blue edge (SDb) (r=-0991) and the reflectance of gr een peak (Rg) (r=-0982), as well as with the reflectance of 530〖KG-*2〗-〖KG-*7〗570 nm ( ︱r︱>095). As a result, the medicinal quality of H. hiemale from Changbai Mountains could be judged rapidly by comparing the SDb and Rg. Fur thermore, to strengthen the incident light of 530〖KG-*2〗-〖KG-*7〗570 nm could stimulate the increase of kaempferol content in Hippochaete hiemale, but it needs to be confirmed by field test.

Key words: AM fungi, Drought tolerance, Protective system, Citrus, Grafting seedling