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生态学杂志 ›› 2005, Vol. ›› Issue (7): 845-847.

• 研究简报 • 上一篇    下一篇

双加氧酶活力对细菌降解菲的指示作用

许华夏1, 李培军1, 巩宗强1, 刘宛1, 钟鸣2, 陈素华1   

  1. 1. 中国科学院沈阳应用生态研究所, 沈阳, 110016;
    2. 沈阳农业大学, 沈阳, 110161
  • 收稿日期:2004-10-24 修回日期:2004-12-16 出版日期:2005-07-10
  • 通讯作者: 许华夏,女,1956年生.主要从事环境污染生态、环境微生物及生态毒理学方面的研究工作,发表论文40余篇.
  • 基金资助:
    国家自然科学基金重点项目(20337010);国家“863”计划项目(2004AA649060);国家自然科学基金资助项目(20277040).

Indicative function of dioxygenase activities on the degradation of phenanthrene by bacteria

XU Huaxia1, LI Peijun1, GONG Zongqiang1, LIU Wan1, ZHONG Ming2, CHEN Suhua 1   

  1. 1. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;
    2. Shenyang Agricultural University, Shenyang 110161, China
  • Received:2004-10-24 Revised:2004-12-16 Online:2005-07-10

摘要: 在液体培养基中选用两种石油降解细菌进行菲降解实验,研究了菲降解率和双加氧酶活力的变化.结果表明,菲降解率受其浓度的影响,当菲浓度为100mg·L-1时,其降解率为最高.而菲浓度高于100mg·L-1时,其降解率下降.实验发现在菲浓度为50~250 mg·L-1条件下,细菌的双加氧酶活力与其菲的降解率存在较好的相关性,对细菌降解菲具有指示作用,可将双加氧酶活力作为菲降解率变化的评价指标.

关键词: 大豆, 蚕豆, 根系, 形态参数, 空间分布

Abstract: Experiments on degradation of phenanthrene by two species of petroleum-degrading bacteria and the change of dioxygenase activities were carried out.The results indicated that when the phenanthrene concentration was 100 mg稬-1, phenanthrenee degradation was the highest.When the phenanthrene concentration was higher than 100 mg稬-1,the degradation decreased.There was a significant correlation between phenanthrene degradation and dioxygenase activities when the phenanthrene concentration ranged from 50 to 250 mg稬-1.The dioxygenase activities could be used as an effective assessment index of phenanthrene degradation process and intensity.

Key words: Soybean, Faba bean, Root system, Configuration parameter, Spatial distribution

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