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生态学杂志 ›› 2004, Vol. ›› Issue (2): 32-36.

• 研究报告 • 上一篇    下一篇

不同碳源对三种溶磷真菌溶解磷矿粉能力的影响

王光华1, 周克琴1, 金剑1, 潘相文1, 赵英2   

  1. 1. 中国科学院东北地理与农业生态研究所, 哈尔滨, 150040;
    2. 黑龙江省科技推广中心, 哈尔滨, 150001
  • 收稿日期:2003-03-01 修回日期:2003-04-30 出版日期:2004-04-10
  • 通讯作者: 王光华,男,1966年生,副研究员.主要从事作物生理生态和环境微生物研究,发表论文30余篇.E-mail:guanghuawang@hotmail.com通讯作者
  • 基金资助:
    国家“十五”科技攻关项目(2001BA007);中国科学院东北地理与农业生态研究所学科前沿领域资助项目(KZCX3-SW-NA-04)

Effect of different C sources on the solubilization of rock phosphate by three phosphate solubilizing fungi (PSF)

WANG Guanghua1, ZHOU Keqin1, JIN Jian1, PAN Xiangwen1, ZHAO Ying2   

  1. 1. Institute of Northeast Geography and Agricultural Ecology, Chinese Academy of Sciences, Harbin 150040, China;
    2. Technology Extension Center of Heilongjiang Province, Harbin 150001, China
  • Received:2003-03-01 Revised:2003-04-30 Online:2004-04-10

摘要: 通过液体培养法,对3种溶磷真菌利用葡萄糖、果糖、蔗糖、麦芽糖、淀粉和纤维素等碳源溶解宜昌产磷矿粉的试验,结果表明,菌株P2.3在供给葡萄糖时的溶磷能力最高,并在一定程度上能够利用长链碳源淀粉和纤维素为营养而溶磷;而高效溶磷菌株P66和P39溶磷的最佳碳源是果糖和麦芽糖,该两菌株利用淀粉和纤维素的溶磷效果很小,甚至不溶磷。3种溶磷真菌培养滤液pH值、可滴定酸含量与其溶磷量之间的相关性因菌株而异,差别很大。菌株P2.3培养滤液pH值、可滴定酸含量与其溶磷量之间相关性很低,但菌株P66和P39培养滤液pH值、可滴定酸含量与其溶磷量之间相关性却达到极显著水平(P <0.01)。结果表明,不同碳源对溶磷菌溶解磷矿粉能力影响很大,分析推断3种菌株产生的有机酸活化磷矿粉能力为P66> P39> P2.3。

关键词: 镉, 牙鲆, 蓄积, 排放, 总有机碳

Abstract: Effect of different C sources (glucose, fructose, sucrose, maltose, starch and cellulose) on solubilization of rock phosphate by 3 phosphate solubilizing fungal strains was studied in liquid media. Strain P2.3 had the highest capacity to solubilize rock phosphate when supplied with glucose, and it also had the ability to use long chain Csources such as starch and cellulose to solubilize rock phosphate. However the strains P66 and P39 had the highest phosphate solubilization activities with fructose and maltose supply. But those strains used a little or no starch and cellulose to solubilize rock phosphate. The relationship among pH, titratable acidity values and soluble Pconcentration of the culture filtrate depended on the fungal strains. When inoculated with P2.3, the relationship among each other was very low. But when using P66 and P39, higher correlations appeared among each other. The experimental results showed that the ability differed in using different Csources to solubilize rock phosphate due to different kinds of organic acids produced by different phosphate solubilizing fungi.The rock phosphate solubilized by organic acids produced by different PSF was P66>P39>P2.3.

Key words: Cadmium, Paralichthys olivaceus, Accumulation, Elimination, Total organic carbon

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