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生态学杂志 ›› 2012, Vol. 31 ›› Issue (01): 106-111.

• 研究报告 • 上一篇    下一篇

太子参连作对根际土壤微生物的影响

林茂兹1,2**,王海斌2,林辉锋2   

  1. 1福建师范大学福清分校生物与化学工程系, 福建福清 350300;2福建农林大学农业生态研究所, 福州 350002
  • 出版日期:2012-01-08 发布日期:2012-01-08

Effects of Pseudostellariae heterophylla continuous cropping on rhizosphere soil microorganisms. 

LIN Mao-zi1,2**, WANG Hai-bin2, LIN Hui-feng2   

  1. 1 Biological and Chemical Department, Fuqing Branch of Fujian Normal University, Fuqing 350300, Fujian, China;2 Institute of Agroecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Online:2012-01-08 Published:2012-01-08

摘要: 采用微生物培养法和末端限制性片断长度多态性(T-RFLP)技术分析连作对太子参根际土壤微生物多样性的影响。结果表明: 连作导致太子参根际土壤细菌和好气性自生固氮菌数量极显著下降,相反,真菌、放线菌、厌气性纤维素分解菌数量极显著增加,而硝化细菌数量变化不显著。T-RFLP分析显示:与太子参-水稻-太子参轮作的土壤相比,太子参连作的土壤细菌种(属)略有减少,其中致病菌和病原菌种(属)增多,并出现一些具拮抗功能的链霉菌属(种);真菌种(属)则表现出上升的趋势,但未检索到与植物致病相关的真菌种(属)。

关键词: 太白红杉, 空间分布, 聚集分布型, 尺度

Abstract: By adopting microbiological culture method and terminal restriction fragment length polymorphism (T-RFLP) technique, this paper analyzed the effects of Pseudostellariae heterophylla continuous cropping on the microbial community structure in the rhizosphere soil. Compared with the rotation system of P. heterophylla-rice-P. heterophylla, the P.heterophylla continuous cropping led to a significant decrease of the amounts of bacteria and aerobic nitrogen-fixing bacteria in rhizospheric soil, but increased the amounts of fungi, actinomycetes, and anaerobic cellulose-decomposing bacteria significantly. No significant change was observed in the amount of nitrifying bacteria. Under P.heterophylla continuous cropping, soil bacterial species (genus) had a slight decrease, in which, the pathogenic and nosogenetic bacteria species (genus) increased, and some Streptomyces genus (species) with antagonistic function were found. The fungi species (genus) had an increasing trend, but none of the plant pathogenic fungi species (genus) was observed.

Key words: Larix chinensis population, Spatial distribution, Clustering pattern type, Scale