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生态学杂志 ›› 2005, Vol. ›› Issue (3): 330-334.

• 测定与方法 • 上一篇    下一篇

植物络合素的测定方法

赵迪1,2, 夏立江1, 朱永官2   

  1. 1. 中国农业大学资源与环境学院, 北京, 100094;
    2. 中国科学院生态环境研究中心, 北京, 100085
  • 收稿日期:2004-03-20 修回日期:2004-06-21 出版日期:2005-03-10
  • 通讯作者: 朱永官
  • 基金资助:
    国家重点基础研究发展规划项目(2002CB410808);国家自然科学基金资助项目(40225002)

Analysis methods of phytochelatins

ZHAO Di1,2, XIA Lijiang1, ZHU Yongguan2   

  1. 1. College of Resource and Environment, China Agricultural University, Beijing 100094, China;
    2. Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
  • Received:2004-03-20 Revised:2004-06-21 Online:2005-03-10

摘要: 植物络合素是一类富含巯基,并能够与重金属离子相结合的多肽.植物络合素具有共同的结构:[γ-Glu-Cys]n Gly(n=2~11).植物络合素是植物重金属抗性的要素之一.植物络合素的测定是一种植物重金属抗性机理的研究技术.介绍了植物络合素测定的理论,并综述了现有的植物络合素测定的方法:分光比色法、凝胶过滤法、液相色谱法和毛细管电泳法.其中,着重阐述了液相色谱法在植物络合素测定中的应用.

关键词: 桧叶金发藓, 泥炭沼泽, 无性系, 固有年际标记, 年龄结构

Abstract: Phytochelatins is a kind of peptides rich in cysteine,which can bind the ion of heavy metals.Phytochelatins has a common structure: [γ-Glu-Cys ]nGly (n=2~11),and it is one of the key factors of plant heavy metal tolerance mechanism.Its analysis methods can be applied to study plant heavy metal tolerance mechanism.The article introduced the basic theory of phytochelatins analysis,and analysis methods including Spectrophotometer,HPLC and CE,among which the HPLC was presented in detail.

Key words: Polytrichum juniperum, Mire, Clone, Innate annual marker, Age structure

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