环境因子对苋菜碳、氮代谢关键酶及抗氧化酶活性的影响

生态学杂志 ›› 2005, Vol. ›› Issue (8): 925-929.

环境因子对苋菜碳、氮代谢关键酶及抗氧化酶活性的影响

隋益虎¹, 朱世东², 张子学¹, 胡德平¹, 舒英杰¹

1. 安徽技术师范学院农学系, 凤阳, 233100;
2. 安徽农业大学园艺系, 合肥, 230036

收稿日期:2004-09-10 修回日期:2004-11-29 出版日期:2005-08-10
通讯作者: 隋益虎,男,1970年生,硕士,副教授.主要从事蔬菜栽培与遗传育种的教学与研究,发表论文20篇.E-mail:suiyihu2004@126.com
基金资助:
安徽技术师范学院稳定人才自然科学基金资助项目(ZRC200433).

Effects of environmental factors on key carbon-nitrogen metabolizing and antioxidizing enzyme activities of Amaranthus

SUI Yihu¹, ZHU Shidong², ZHANG Zixue¹, HU Deping¹, SHU Yingjie¹

1. Department of Agronomy, Anhui Technical Teachers College, Fengyang 233100, China;
2. Department of Horticulture, Anhui Agricultural University, Hefei 230036, China

Received:2004-09-10 Revised:2004-11-29 Online:2005-08-10

摘要/Abstract

摘要： 研究了塑料大棚内光照强度、气温、地温、二氧化碳浓度及氮肥施量等5个环境因子、5个水平处理组合对春苋菜早熟栽培生理的影响。结果表明,在碳、氮代谢关键酶方面,不同氮水平下,光强、气温与硝酸还原酶(NR)活性(极)显著正相关,各处理组合中其活性次序为A(0网0膜)>B(0网1膜)>C(1网1膜)>D(2网1膜)>E(3网1膜),后4者分别比处理A(对照)降低了6.45%、34.21%、61.54%和63.78%;CO₂浓度、气温和地温(极)显著影响磷酸烯醇式丙酮酸羧化酶(PEPC)活性,处理B的PEPC平均活性最强,处理B、C、D、E的PEPC活性较A分别增加了59.69%、28.57%、-10.71%和-17.86%。在活性氧代谢方面,超氧化物歧化酶(SOD)活性与光强正相关,与CO₂浓度负相关,大小表现为A>B>C>D>E;过氧化物酶(POD)活性与光强、气温(极)显著负相关,为A<B<C<D<E;过氧化氢酶(CAT)活性与光强、气温(极)显著正相关,为B>A>C>D>E。POD在处理A、B、C中起保护作用,而在D、E中可能起伤害作用。各环境因子组合中,B(0网1膜:平均光强17800lx、地温21.2℃、气温23.5℃及CO₂浓度1500μmol·mol^-1)在施氮水平为N₅(20kg·667m^-2)时,各酶活协调性最好,可以实现苋菜的高产、高效和优质栽培。

关键词: 硝酸镧, ICPMS, 微核率, DNA断裂, 遗传毒理

Abstract: This paper studied the physiological indexes of Amaranthus cultivated in a big vinyl house in early spring under different combinations of five levels of sunlight intensity,air temperature,soil temperature,carbon dioxide concentration,and nitrogen fertilization.The results showed that for carbon and nitrogen metabolism,nitrate reductase activity (NRA) had significantly positive correlations with sunlight intensity and air temperature at different nitrogen application levels,and the order was A (no net and no film)>B (no net and one layer film)>C (one layer net and one film)>D (two layers net and one film)>E (three layers net and one film).Compared with A (control),the NRA of the other four treatments was decreased by 6.45%,34.21%,61.54% and 63.78% respectively.CO₂ concentration and air and soil temperature affected the activity of PEPC significantly.Of all treatments,the PEPC activity in treatment B was the highest,and that of B,C,D and E was 59.69%,28.57%,-10.71% and -17.86% higher than A,respectively.For the metabolism of active oxygen system,SOD activity was positively correlated with sunlight,but negatively correlated with CO₂ concentration,and the order was A>B>C>D>E.POD activity was negatively correlated with sunlight and air temperature,with the order of A<B<C<D<E,and CAT was positively correlated with sunlight and air temperature,with the order of B>A>C>D>E in all combinations.The enzyme activities of B with 20 kg·667 m^-2,17 800 lx sunlight intensity,21.2 ℃ soil temperature,23.5℃ air temperature and 1 500 μmol CO₂·mol^-1 were the most cooperative,which made the Amaranthus cultivation technical system of "high yield,high efficiency and excellent quality" be possible.

Key words: La(NO3)3, ICP-MS, Micronucleus rate, DNA cleavage, Genetic toxicity

中图分类号:

S636.4

隋益虎, 朱世东, 张子学, 胡德平, 舒英杰. 环境因子对苋菜碳、氮代谢关键酶及抗氧化酶活性的影响[J]. 生态学杂志, 2005, (8): 925-929.

SUI Yihu, ZHU Shidong, ZHANG Zixue, HU Deping, SHU Yingjie. Effects of environmental factors on key carbon-nitrogen metabolizing and antioxidizing enzyme activities of Amaranthus[J]. cje, 2005, (8): 925-929.

参考文献

[1] 山东农学院,西北农学院.1982.植物生理学实验指导[M].济南:山东科学技术出版社,109～112.
[2] 马瑞昌,宋书娟,喻梅辉.1998.冰草属牧草硝酸还原酶的活性与其产量营养的关系[J].中国草地,(2):52～58.
[3] 王羽梅,任安祥,潘春香,等.2003.不同浓度NaCl对苋菜幼苗生长、光合速率、离子吸收和甜菜碱含量的影响[J].植物生理学通讯,39(1):13～16.
[4] 卢纹岱.2003.SPSS for Windows统计分析(第2版)[M].北京:电子工业出版社,145～249.
[5] 华东师范大学.1983.植物生理学实验指导[M].北京:人民教育出版社,73～75.
[6] 孙谷畴.1988.苋菜的光合特性[J].广西植物,8(3):279～284.
[7] 余让才,李明启,范燕萍.1997.高等植物硝酸还原酶的光调控[J].植物生理学通讯,33(1):61～65.
[8] 吴时敏.1999.苋菜的开发利用[J].农村实用工程技术,(3):29～30.
[9] 宋建民,田纪春,赵世杰.1998.植物光合碳和氮代谢之间的关系及其调节[J].植物生理学通讯,34(3):230～236
[10] 张文军,葛超.2000.NBT光还原法、邻苯三酚自氧化法测定SOD酶活性的比较[J].河北职业技术师范学院学报,14(2):68～70.
[11] 杨淑慎,高俊凤.2001.活性氧、自由基与植物的衰老[j] .西北植物学报,21(2):215～220.
[12] 肖深根,刘志敏,宋勇,等.2000.苋菜品种资源光合特性研究[J].长江蔬菜,(10):33～35.
[13] 肖深根,刘志敏,宋勇,等.2000.苋菜品种资源的分类研究[J].湖南农业大学学报,26(4):274 277.
[14] 林植芳,林桂珠,李双顺.1988.苋菜叶片和叶绿体消除活性氧能力的变化[J].植物生理学通讯,24(5):28～31.
[15] 林植芳,彭长连,林桂珠.2000.活性氧对苋菜磷酸烯醇式丙酮羧化酶活性的影响[J].植物生理学报,26(1):27～32.
[16] 林植芳.1998.叶龄对苋菜光合作用特性的影响[J].植物学通报,15(1):41～44.
[17] 武之新,徐玉鹏.1997.苋属作物在沧州区域的生态适应性[J].生态学杂志,16(1):63～64.
[18] 施教耐,吴敏贤,查静娟.1979.植物磷酸烯醇式丙酮羧化酶的研究[J].植物生理学报,5(3):225 235.
[19] 焦进安.1987.植物磷酸烯醇式丙酮羧化酶的多种生理功能[J].植物生理学通讯,23(1):40～43.
[20] Ayodele Ⅵ. 2000. Influence of soil water stress at different physiological stages on growth and seed yield of Amaranthus species[J]. Acta Hortic., 537 (2): 767 ～ 772.
[21] Candella MI, Fishet EG, Hewitt EF. 1957. Some factors affecting the activity of nitrate reductase in cauliflower plants grown with different nitrogen sources and molybdenum levels in sand culture[J]. Plant Physiol., 32: 280～ 288.
[22] Gaikwad DK, Chavan PD. 1999. Effect of salinity on carbohydrate status of three Amaranthus species[J]. Acta Bot. Hungarica,42(1):111～117.
[23] Parvathi K. 2000. Illumination increase the affinity of phyosphoenolpyruvate carboxylase to bicarbonate in leaves of a C4 plant,Amaranthus h ypochondriacus [J]. Plant Cell Physiol., 41 (8):905 ～910.
[24] Matta FM, Loos RA, Rodrigues R, et al. 2001. Actual and potential photosynthetic rates of tropical crop species [J]. Rev.Brasileira de Fisiol . Veget ., 13(1) :24～32.
[25] Serri GH, Berti DM, Vidal P, et al. 2000. Response to nitrogen fertilizer and nitrogen use efficiency in Amaranth [J]. Cienciae Investigat . Agraria, 26(3): 155 ～ 162.