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生态学杂志 ›› 2009, Vol. 28 ›› Issue (08): 1489-1493.

• 研究报告 • 上一篇    下一篇

广东北江流域部分野生淡水鱼类种质资源调查

黄志坚;何建国;唐晶晶;徐晓鹏;郑锦卿   

  1. 中山大学海洋学院/生命科学学院
  • 出版日期:2009-08-10 发布日期:2009-08-10

Germplasm resource investigation of wild freshwater fish in Beijiang River of Guangdong.

HUANG Zhi-Jian;HE Jian-Guo;TANG Jing-Jing;XU Xiao-Feng;ZHENG Jin-Qing   

  1. School of Marine Sciences,School of Life Sciences,Sun Yat-sen University
  • Online:2009-08-10 Published:2009-08-10

摘要: 为了解广东省北江流域野生淡水鱼类种质资源和遗传多样性,于2007—2008年开展了广东省北江流域部分野生淡水鱼类的调查,运用线粒体DNA D-loop测序方法开展遗传多样性分析,测定采集鱼类的mtDNA D-loop基因序列并提交GenBank数据库,运用相关软件分析序列并建立聚类树。采集的野生淡水鱼类32种,隶属于3目10科27属,其中鲤形目鱼类21种,占65.6%,鲈形目鱼类4种,占12.5%,鲇形目鱼类7种,占21.9%。在鲤形目鱼类中,鳅科、平鳍鳅科、爬鳅科共12种,占鲤形目鱼类的57.1%。调查结果表明,北江流域存在丰富的野生淡水鱼类物种多样性,须采取有效措施保护北江流域淡水鱼类资源。测定的线粒体DNA D-loop序列均提交GenBank数据库获得序列号:EU380208-EU380236,EU697088-EU697148。测序结果和聚类树分析表明,线粒体DNA D-loop可作为鱼类种类鉴定的分子标记,分析结果印证了传统形态分类学的知识,提供了分子生物学证据。

关键词: 植被制图, 3S, 混淆矩阵, 精度分析, 梅里雪山

Abstract: In order to understand the germplasm resources and genetic diversity of wild freshwater fish in Beijiang River of Guangdong Province; an investigation was made in 2007-2008. Thirty-two species of wild freshwater fish were collected; belonging to three orders; ten families; and twenty-seven genera; among which; there were twenty-one species of Cypriniformes; four species of Perciformes; and seven species of Siluriformes. Of the twenty-one species of Cypriniformes; there were twelve species belonging to Cobitidae; Homalopteridae; Gastromyzonidae; and Balitoridae. The mtDNA D-loop sequences of test freshwater fish were determined; and submitted to GenBank (Accession numbers EU380208-EU380236 and EU697088-EU697148). Molecular phylogenetic trees were constructed by using the maximum parsimony method. All the results showed that Beijiang River had abundant freshwater fish resource; and mtDNA D-loop could be a good molecular marker for the fish species identification.

Key words: Vegetation map, 3S technology, Error matrix, Accuracy analysis, Meili Snow Mountain