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生态学杂志 ›› 2009, Vol. 28 ›› Issue (12): 2574-2578.

• 研究报告 • 上一篇    下一篇

含油废水处理工艺中细菌多样性

李凤梅1,2;黄翠霞3;郭书海1**;张玲妍1   

  1. 1中国科学院沈阳应用生态研究所|沈阳 110016;2中国科学院研究生院|北京 100039;3铁岭师范高等专科学院|辽宁铁岭 112000
  • 出版日期:2009-12-10 发布日期:2009-12-10

Bacterial diversity in oil wastewater during its treatment process.

LI Feng-mei1,2|HUANG Cui-xia3;GUO Shu-hai1|ZHANG Ling-yan1   

  1. 1Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China|2Graduate University of Chinese Academy of Sciences, Beijing 100039, China;3Tieling Teachers College, Tieling 112000, Liaoning, China
  • Online:2009-12-10 Published:2009-12-10

摘要: 从辽河油田曙光污水处理厂含油废水样品中直接抽提细菌总DNA,并对总DNA中16S rRNA V3可变区序列作PCR扩增、变性梯度凝胶电泳(DGGE),与常规水质分析相结合对系统中不同处理阶段细菌种群多样性和水质之间的关系进行分析。结果表明,处理系统的不同处理阶段细菌的多样性有明显差异,既存在共同的细菌种属,也存在着各自独特的细菌种属,且细菌种群的多样性与水质CODcr和总石油烃(TPH)的浓度呈负相关,细菌种群的多样性越高,CODcr和TPH的浓度越低,反之则越高。在处理过程中,随着样品中细菌多样性的增加,种群结构之间的相似性指数(Cs)逐渐升高,最后形成了稳定的种群结构。

关键词: 土壤微生物, 土壤酶活性, 施肥

Abstract: The total DNA was directly extracted from the oil wastewater of Shuguang Wastewater Treatment Plant in Liaohe Oilfield, and by the methods of PCR-DGGE and conventional water quality analysis, the relationships between the bacterial diversity in the wastewater during its treatment process and the water quality were analyzed. There were significant differences in the bacterial communities at different treatment stages, not only some common bacterial species, but also the unique bacteria population. The bacterial diversity was negatively correlated with the CODcr and TPH concentrations in wastewater. The higher the CODcr and TPH concentrations, the lower the bacterial diversity was. During treatment process, the similarity coefficient (Cs) of bacterial communities in the wastewater increased with enhancing bacterial diversity, and finally formed a stable population structure.

Key words: Soil microbial biomass, Soil enzyme activity, Fertilization