关键词: 黄瓜, 抗寒性, 三唑酮, 生物量, 膜脂过氧化, 脯氨酸

Abstract: Accurate detection of populations virus-carrying rate and individuals rice stripe virus (RSV) content of small brown planthopper (Laodelphax striatellu) is one of the important references to predict whether the rice stripe disease could outbreak under elevated CO₂ concentration. In this study, qualified RT-PCR assay (qRT-PCR) was employed to detect the RSV content in L. striatellu under different CO₂ concentrations. Among the test three CO₂ concentrations (370, 470, and 570 μl·L^-1), 470 μl·L^-1 of CO₂ was most favorable to the multiplication of RSV in L. striatellu. Compared with the commonly used ELISA (enzyme-linked immunosorbnent assay) and DIBA (dot immunobinding assay), qRT-PCR was more rapid, sensitive, and specific, and didn’t need antiserum preparation, being easily to be popularized.

Key words: Cucumber, Chilling injury resistance, Triadimefon, Biomass, Membrane lipid peroxidation, Pro-line