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生态学杂志 ›› 2005, Vol. ›› Issue (1): 6-8,96.

• 研究报告 • 上一篇    下一篇

重金属铜抗性菌株的筛选及其生物学特性的研究

江春玉, 盛下放, 夏娟娟   

  1. 南京农业大学生命科学学院农业部农业环境微生物工程重点开放实验室, 南京, 210095
  • 收稿日期:2003-10-12 修回日期:2003-12-12 出版日期:2005-01-10
  • 通讯作者: 盛下放
  • 基金资助:
    国家自然科学基金项目(40371070);江苏省社会发展重点资助项目(BS2002044)

Screening of copper-resistance strains and their biological characters

JIANG Chunyu, SHENG Xiafang, XIA Juanjuan   

  1. Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2003-10-12 Revised:2003-12-12 Online:2005-01-10

摘要: 通过在培养基中加入一定浓度的Cu(CuSO4·5H2O,Cu200mg·L-1),从42份土壤样品中筛选得到2株具有很强Cu抗性的细菌B和G,经初步鉴定它们分别属于无色细菌属和芽胞杆菌属。将菌株B和G接种到添加Cu(150mg·L-1)的培养液中进行沉淀态铜的活化试验,结果表明,与不接菌对照相比,B和G菌株分别使培养液中有效铜含量增加475.5%和249.5%,培养液pH由6.5分别降低到4.2和5.0,菌株B和G的活化效能与其代谢产酸有关。B菌株和G菌株最适生长温度为28℃,最适pH分别为6~7和8,G菌株在5%NaCl下生长良好。除抗Cu外,两株菌对重金属Pb(400mg·L-1)、Cd(100mg·L-1)也具抗性。

关键词: 生物安全, 转基因逃逸, 生态风险, 生物多样性

Abstract: Copper-resistance (Cu 200 mg·L-1) strains B and G were screened out from 42 soil samples,which were primarily identified as Achromobacter and Bacillus.Strain B and G were inoculated to the solution containing Cu (150 mg·L-1 ) for activation tests.The results showed that in the liquid medium inoculated strain B and G,the contents of Cu2+ increased by 475.5% and 249.5% respectively compared with the control,and pH decreased from 6.5 to 4.2 and 5.0,respectively.The activation efficiency of the bacteria correlated to their ability to produce acid.The optimum growth conditions for them were: temperature 28 ℃ for both strain B and G,pH 6~7 for strain B and 8 for strain G.Strain G grew well in the medium containing 5% NaCl.Moreover,strain B and G can also be resistant to Pb2+ (400 mg·L-1 ) and Cd 2+ (100 mg·L-1).

Key words: Biosafety, Transgene escape, Ecological risk, Biodiversity

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