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生态学杂志 ›› 2011, Vol. 30 ›› Issue (02): 315-319.

• 研究报告 • 上一篇    下一篇

多环芳烃降解菌的筛选与降解能力测定

袁 爽1梁成华1**李凤梅2,3郭书海2   

  1. 1沈阳农业大学, 沈阳 110866;2中国科学院沈阳应用生态研究所, 沈阳 110016;3中国科学院研究生院,北京 100049
  • 出版日期:2011-02-08 发布日期:2011-02-08

Screening of PAHs-degrading bacterial strains and determination of their capability in degrading PAHs.

YUAN Shuang1, LIANG Cheng-hua1**, LI Feng-mei2,3, GUO Shu-hai2   

  1. 1Shenyang Agricultural University, Shenyang 110866, China|2Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China|3Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • Online:2011-02-08 Published:2011-02-08

摘要: 从本溪多环芳烃(PAHs)污染土壤中经富集培养筛选出8株PAHs降解菌,研究了8株菌及其等比例混合培养对菲、芘和苯并[a]芘的降解能力。结果表明,在28 ℃,培养基中菲、芘和苯并[a]芘的浓度分别为50、50和5 mg·L-1的复合底物条件下,培养28 d后,菌株B3的降解效果最好,对菲、芘和苯并[a]芘的降解率分别为88.4%、54.0%和68.4%,8株菌的混合培养对菲、芘和苯并[a]芘的降解率分别为87.7%、35.3%和42.0%;经生理生化实验和16S rRNA序列比对,初步鉴定B3菌为假单胞菌属(Pseudomonas sp.)。

关键词: 潮间带, 群落多样性, 群落, 长山列岛

Abstract: Eight PAHs-degrading bacterial strains were screened out from the enriched culture with PAHs-contaminated soils in Benxi City of Liaoning Province, Northeast China, and the capability of the strains in degrading phenanthrene, phyrene, and benzo[a]pyrene with an initial concentration of 50, 50, and 5 mg·L-1 in medium was determined after 28-day incubation at 28 ℃. Among the strains screened out, B3 showed the best degrading effect, with the degradation rate of phenanthrene, phyrene, and benzo[a]pyrene being 88.4%, 54.0%, and 68.4%, while the degradation rate of these compounds by the mixed 8 strains was 87.7%, 35.3%, and 42.0%, respectively. The strain B3 was identified as Pseudomonas sp., based on its physiological and biochemical characteristics and the sequence analysis of 16S rRNA.

Key words: Intertidal zone, Community diversity, Community, Changshan archipelago