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生态学杂志 ›› 2011, Vol. 30 ›› Issue (08): 1620-1626.

• 研究报告 • 上一篇    下一篇

外源NO对NaCl胁迫下长春花幼苗生物量和叶绿素荧光的影响

胡凡波,刘玲,隆小华,刘兆普   

  1. 南京农业大学资源与环境科学学院 江苏省海洋生物学重点实验室, 南京 210095
  • 出版日期:2011-08-08 发布日期:2011-08-08

Effects of exogenous nitric oxide on biomass and chlorophyll fluorescence of Catharanthus roseus seedlings under NaCl stress.

HU Fan-bo, LIU Ling, LONG Xiao-hua, LIU Zhao-pu**   

  1. Jiangsu Province Key Laboratory of Marine Biology, College of Resources and Environmental Science, Nanjing Agricultural University, Nanjing 210095, China
  • Online:2011-08-08 Published:2011-08-08

摘要: 在温室条件下采用盆栽法,研究了50 mmol·L-1 NaCl胁迫下5个不同浓度外源NO供体硝普钠(sodium nitroprusside,SNP)对长春花(Catharanthus roseus)幼苗生物量、叶绿素含量和叶绿素荧光的影响。结果表明:(1) 鲜重和干重均在S2(50 mmol·L-1 NaCl+0.1 mmol·L-1 SNP)处理下达到最高,分别较对照S0(50 mmol·L-1 NaCl)显著增加18.8%和13.9%。叶绿素a、叶绿素b和类胡萝卜素与生物量相似,均在S2处理下含量最高,但它们均与S0无显著差异,叶绿素a/b变化较为复杂。(2) 0.1 mmol·L-1 SNP缓解盐胁迫对PSⅡ反应中心的破坏效果最好,显著提高50 mmol·L-1 NaCl胁迫下长春花幼苗叶片的可变荧光(Fv)、最大荧光(Fm)、PSⅡ最大光化学效率(Fv/Fm)、PSⅡ潜在活性(Fv/Fo)、实际光化学效率(φPSⅡ)和光化学荧光猝灭系数(qP),降低了初始荧光(Fo)、非光化学荧光猝灭系数(qN)。综上所述,0.1 mmol·L-1 SNP能够通过降低盐胁迫对叶绿素的降解和对PSⅡ反应中心的破坏,促进幼苗生长,提高对50 mmol·L-1 NaCl的抗逆性。

关键词: 大麦, 镉, 养分, 生长, 品种

Abstract: A pot experiment in greenhouse was carried out to study the effects of different concentration exogenous NO donor (sodium nitroprusside, SNP) on the biomass, chlorophyll content, and chlorophyll fluorescence of Catharanthus roseus seedlings under the stress of 50 mmol NaCl·L-1. Among the treatments with SNP, the seedlings treated with 50 mmol NaCl·L-1 + 0.1 mmol SNP·L-1 had the highest  fresh weight and dry weight, being significantly higher (18.8% and 13.9%, respectively) than the control (50 mmol NaCl·L-1). Similar to biomass, the chlorophyll a, chlorophyll b, and carotenoid contents in treatment 50 mmol NaCl·L-1 + 0.1 mmol SNP·L-1 were the highest, but had no significant differences with the control. The variation trend of chlorophyll a/b was more complicated. Applying 0.1 mmol·L-1 of SNP had the best effect in mitigating the salt damage to PSⅡreaction center, which increased the fluorescence (Fv), maximal fluorescence (Fm), light energy transformation efficiency of PSⅡ (Fv/Fm), activity potential of PSⅡ (Fv/Fo), actual photochemical efficiency of PSⅡ (φPSⅡ), and photochemical quenching (qP) markedly, and decreased the minimal fluorescence (Fo) and non-photochemical quenching (qN) obviously. To sum up, 0.1 mmol·L-1 of SNP had the best effect in protecting chlorophyll and PSⅡ reaction center against salt damage, and also, could promote the growth of the seedlings and enhance their tolerance to 50 mmol·L-1 NaCl stress.

Key words: Barley (Hordeum vulgare), Cadmium, Nutrients, Growth, Cultivar