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大气O3浓度升高对毛竹光合生理的影响

李迎春1,李应1,2,陈双林1**,庄明浩1,郭子武1,杨清平1   

  1. (1中国林业科学研究院亚热带林业研究所, 浙江富阳 311400; 2贵州大学林学院, 贵阳 550025)
  • 出版日期:2013-02-10 发布日期:2013-02-10

Impacts of elevated atmospheric ozone concentration on the photosynthetic physiology of Phyllostachys edulis.

LI Ying-chun1, LI Ying1,2,CHEN Shuang-lin1**, ZHUANG Ming-hao1, GUO Zi-wu1, YANG Qing-ping1   

  1. (1 Research Institute of Subtropical Forestry, Chinese Forestry Academy, Fuyang 311400, Zhejiang, China; 2 Forestry College, Guizhou University, Guiyang 550025, China)
  • Online:2013-02-10 Published:2013-02-10

摘要: 以毛竹(phyllostachys edulis)为试材,运用开顶式气室(OTCs)模拟了4个大气O3浓度情景,分别为CF(背景大气经活性炭过滤后的处理,22~25 nL·L-1)、NF(直接将背景大气输入气室内,40~45 nL·L-1)、T1(O3平均浓度100 nL·L-1,92~106 nL·L-1)、T2(O3平均浓度150 nL·L-1,142~160 nL·L-1),分析大气O3浓度对毛竹叶片光合色素、气体交换参数、光合参数的影响。结果表明:不同浓度O3处理的毛竹叶片PnTr日变化均呈单峰型,随着O3浓度的升高,叶片GsCiLs日变化规律趋于简单,WUE变化趋于平缓。与环境背景大气比较,高浓度O3(≥100 nL·L-1)条件下叶片PnLsWUE日均值和Chl、Chla、Chlb和Car含量均显著降低,GsTr日均值显著提高,但对Ci日均值及叶片光合色素组成影响并不明显。T1、T2处理下,毛竹的PnTrPPED、VpdLTairCaRH环境因子间均呈显著或极显著相关,GsPPED无显著相关,而与VpdLTairCaRH环境因子间均呈显著或极显著相关。研究表明:O3浓度100、150 nL·L-1时分别会造成毛竹叶片发生气孔、非气孔限制,气孔对环境条件的反应变得不敏感,影响了正常的调节反馈机制,增加了水分蒸散,光合色素降解或合成受阻,对毛竹光合作用产生严重的负面效应,不利于毛竹干物质积累。

关键词: 堆腐系统, 循环率, 有机肥N、P利用率

Abstract: In this study, open-top chambers (OTCs) were employed to approach the impacts of elevated atmospheric ozone (O3) concentration on the photosynthesis of Phyllostachys edulis. Five treatments were installed, i.e., CF (background atmosphere after filtrated by activated carbon, 22-25 nL·L-1), NF (directly introduce the background atmosphere to the gas chamber, 40-45 nL·L-1), T1 (92-106 nL·L-1 of O3, averagely 100 nL·L-1), T2 (142-160 nL·L-1 of O3, averagely 150 nL·L-1). Under different O3 concentrations, the diurnal variations of P. edulis leaf Pn and Tr represented onepeak curves. With the increasing concentration of O3, the diurnal variations of the leaf Gs, Ci, and Ls trended to be simplified, while that of WUE became stable gradually. Under higher concentrations (≥ 100 nL·L-1) O3, the diurnal means of Pn, Ls, and WUE and the contents Chl, Chl-a, Chl-b, and Car decreased significantly, those of Gs and Tr had a significant increase, while the diurnal mean of Ci and the composition of photosynthetic pigment changed less. In treatments T1 and T2, the Pn,Tr, and Gs were significantly correlated with the environmental factors VpdL, Tair, Ca, and RH, and the Pn and Tr were significantly correlated with the PPED while the Gs was less correlated with the PPED. All the results indicated that 100 and 150 nL·L-1 of O3 could induce the leaf stomatal or nonstomatal limitation of P. edulis, respectively, and the stoma became insensitive to the environment, which impacted the leaf’s normal feedback mechanisms, increased leaf evapotranspiration, and inhibited the degradation or synthesis of photosynthetic pigments, exerting serious negative effects on the photosynthesis and dry matter accumulation of P. edulis.