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一氧化氮在微波预处理提高小麦幼苗抗镉胁迫中的作用

邱宗波**,张曼曼,郭君丽,雷梦云   

  1. (河南师范大学生命科学学院, 河南新乡 453007)
  • 出版日期:2013-07-10 发布日期:2013-07-10

Role of nitric oxide in improving cadmium tolerance of microwave-pretreated wheat seedlings.

QIU Zong-bo**, ZHANG Man-man, GUO Jun-li, LEI Meng-yun   

  1. (College of Life Science, Henan Normal University, Xinxiang, Henan 453007, China)
  • Online:2013-07-10 Published:2013-07-10

摘要: 为了探讨一氧化氮在微波预处理提高小麦抗镉胁迫中的作用,利用微波(126 mW·cm-2)辐照小麦种子10 s,待其长至一叶一心时,用150 μmol·L-1 CdCl2胁迫其幼苗,并通过添加一氧化氮专一清除剂cPTIO(0.5%,w/v),研究一氧化氮对微波预处理小麦幼苗脂质过氧化、抗氧化酶活性、光合色素含量及幼苗生长的影响。结果表明:微波预处理可使镉胁迫的小麦幼苗丙二醛含量显著降低(P<0.05),超氧化物歧化酶、过氧化物酶、过氧化氢酶活性、一氧化氮、叶绿素a、叶绿素b、类胡萝卜素含量和株高、根长、地上部干重、根干重显著增加(P<0.05);经过适当剂量微波辐照镉胁迫小麦幼苗再加外源cPTIO处理能有效逆转微波对镉胁迫小麦幼苗的防护作用。说明微波对镉胁迫损伤小麦幼苗起到一定的防护作用主要是通过NO起作用。

关键词: 转基因作物, Bt, 毒素蛋白, 土壤性质

Abstract: To approach whether nitric oxide (NO) in microwave pretreatment can improve wheat seedling’s cadmium tolerance, wheat seeds were pretreated with microwave radiation (126 mW·cm-2)  for 10 s, and the seedlings were treated with 150 μmol·L-1 of CdCl2 and 0.5% (w/v) of cPTIO (a NO scavenger), aimed to study the effects of NO on the lipid peroxidation, antioxidant enzyme activities, photosynthesis pigment concentrations, and growth of microwavepretreated wheat seedlings under cadmium stress. The 10 s microwave pretreatment could significantly decrease the malondialdehyde content, while increase the superoxide dismutase, peroxidase, catalase activities, the concentrations of nitric oxide, chlorophyll a, chlorophyll b, and carotenoid, and the plant height, root length, and shoot and root dry weight of the seedlings under cadmium stress. The amelioration effect of the microwave pretreatment on the cadmium stress was effectively reversed by the addition of cPTIO, suggesting that NO was involved in the microwave pretreatment-induced cadmium tolerance of wheat seedlings, and microwave pretreatment did trigger NO production which could lead to the cadmium tolerance of wheat seedlings.