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玻璃化法超低温保存对大叶藻种子活力的影响

张凌宇,张沛东**,田璐,孙燕,张秀梅   

  1. (中国海洋大学水产学院, 山东青岛 266003)
  • 出版日期:2013-02-10 发布日期:2013-02-10

Effects of cryopreservation by vitrification on the viability of eelgrass Zostera marina seeds.

ZHANG Ling-yu, ZHANG Pei-dong**, TIAN Lu, SUN Yan, ZHANG Xiu-mei   

  1. (College of Fisheries, Ocean University of China, Qingdao 266003, Shandong, China)
  • Online:2013-02-10 Published:2013-02-10

摘要: 通过设置玻璃化法超低温保存的3个关键要素,即冰冻保护液处理、玻璃化溶液脱水时间和冷冻处理,研究了玻璃化法超低温保存对大叶藻种子活力的影响。结果表明:冰冻保护液处理和玻璃化溶液脱水时间对大叶藻种子活力无显著影响;冷冻处理组种子活力显著低于对照组,而冰冻保护液和玻璃化脱水处理对冷冻后种子活力的影响不显著,表明冰冻保护液和玻璃化溶液未对种子起到明显的保护作用;未冰冻保护液处理组玻璃化溶液脱水4 h时种子活力显著低于其他脱水时间,而冰冻保护液处理组脱水4 h时与其他脱水时间无明显不同,表明较长时间的玻璃化溶液脱水处理需使用冰冻保护液进行预处理。研究结果为超低温保存大叶藻种子提供了参考。

关键词: 有机胶结物质, 土壤团聚体, 理论模型

Abstract: In this paper, three key factors of cryopreservation by vitrification, i.e., cryoprotective solution application, dehydration time of vitrification solution, and rapid freezing, were installed to study the effects of cryopreservation by vitrification on the viability of Zostera marina seeds. Cryoprotective solution application and dehydration time had no significant effects on the viability of the seeds. When exposed to rapid freezing, the viability of the seeds was significantly lower than that of the control, while cryoprotective solution application and dehydration time showed no significant protective effects on the viability of the seeds. Without cryoprotective solution application, the viability of the seeds subjected to 4 h dehydration was significantly lower than that of the seeds subjected to other lengths of dehydration time. With cryoprotective solution application, no significant difference was found between the viability of the seeds subjected to 4 h dehydration and that of the seeds subjected to other lengths of dehydration time. These findings indicated the necessity of cryoprotective solution application to seeds prior to the long period dehydration by vitrification. This study provides a reference for the cryopreservation of Z. marina seeds.