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生态学杂志 ›› 2011, Vol. 30 ›› Issue (10): 2173-2178.

• 研究报告 • 上一篇    下一篇

外源一氧化氮供体硝普钠对黑麦草幼苗耐碱性的影响

刘建新**,王鑫,王瑞娟,贾海燕   

  1. 陇东学院生命科学与技术学院, 甘肃省高校陇东生物资源保护与利用省级重点实验室, 甘肃庆阳 745000
  • 出版日期:2011-10-08 发布日期:2011-10-08

Effects of exogenous nitric oxide donor sodium nitroprusside on alkali resistance of Lolium perenne seedlings.

LIU Jian-xin**,WANG Xin, WANG Rui-juan, JIA Hai-yan   

  1. College of Life Science and Technology, Longdong University/University Provincial Key Laboratory for Protection and Utilization of Longdong Bio-resources in Gansu Province, Qingyang 745000, Gansu, China
  • Online:2011-10-08 Published:2011-10-08

摘要: 采用营养液砂培方法,研究外源一氧化氮(NO)供体硝普钠(SNP)对NaHCO3胁迫下黑麦草幼苗生长、活性氧代谢和渗透溶质积累的影响。结果表明:60 μmol·L-1 SNP能够缓解100 mmol·L-1NaHCO3 迫对黑麦草幼苗生长的抑制作用,减缓NaHCO3胁迫导致的叶片O2产生速率、H2O2和丙二醛(MDA)含量的增加,提高NaHCO3胁迫下幼苗叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)、质膜H+-ATP酶的活性和谷胱甘肽(GSH)、可溶性糖、可溶性蛋白质和脯氨酸的含量及K+/Na+比,降低过氧化氢酶(CAT)活性和抗坏血酸(AsA)含量,对游离氨基酸含量影响不大。上述结果表明,NO可能通过激活抗氧化系统活性、促进渗透溶质积累和改善Na+、K+平衡等缓解碱胁迫对幼苗的伤害,从而提高黑麦草的耐碱性。

关键词: 重金属污染土壤, 物理化学技术, 植物修复

Abstract: A sand culture experiment was conducted to study the effects of nitric oxide (NO) donor sodium nitroprusside (SNP) on the growth, leaf active oxygen metabolism, and leaf osmotica accumulation of ryegrass (Lolium perenne L.) seedlings under aHCO3 stress. Under the stress of 100 mmol NaHCO3·L-1, applying 60 μmol·L-1 of SNP could alleviate the seedling’s growth inhibition, increase the leaf O2 generation rate and H2O2 and malondialdehyde contents, promote the leaf superoxide dismutase, guaiacol peroxidase, ascorbate peroxidase, and plasma membrane H+-ATPase activities, increase the leaf lutathione, soluble sugar, soluble protein, and proline contents and K+/Na+ ratio, and decrease the leaf catalase activity and ascorbic acid content. Little effects were observed on the leaf free amino content. These results suggested that SNP could alleviate the damage of alkali stress on Lolium perenne eedling leaves via activating the antioxidant system activity, promoting the osmotica accumulation, and improving the Na+ and K+ balance, and accordingly, promote the alkali resistance of the ryegrass plant.

Key words: Heavy metal contaminated soil, Physio-chemical technique, Phytoremediation