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生态学杂志 ›› 2011, Vol. 30 ›› Issue (10): 2197-2202.

• 研究报告 • 上一篇    下一篇

外源亚精胺对高温胁迫下黄瓜幼苗氮素代谢的影响

田婧,郭世荣**,孙锦,王丽萍,阳燕娟,李斌   

  1. 南京农业大学园艺学院, 农业部南方蔬菜遗传改良重点开放实验室, 南京 210095
  • 出版日期:2011-10-08 发布日期:2011-10-08

Effects of exogenous spermidine on nitrogen metabolism of cucumber seedlings  under high temperature stress.

TIAN Jing, GUO Shi-rong**, SUN Jin, WANG Li-ping, YANG Yan-juan, LI Bin   

  1. College of Horticulture, Nanjing Agricultural University; Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, Nanjing 210095, China
  • Online:2011-10-08 Published:2011-10-08

摘要: 以较为耐热的黄瓜品种‘津春4号’为试材,在人工气候箱中,采用石英砂培加营养液浇灌的栽培方式,研究了外源亚精胺(Spd)对高温胁迫(42 ℃)下黄瓜幼苗氮素代谢的影响。结果表明:短期高温胁迫处理,尤其是4 h内,植株硝态氮含量降低而铵态氮含量升高;外源Spd预处理使幼苗体内硝态氮和铵态氮含量升高且硝酸还原酶(NR)活性增强。较长期高温胁迫处理下,幼苗根系中硝态氮含量升高但向地上部运输受阻,根系NR钝化,根系和叶片中铵态氮含量均显著升高;高温胁迫下喷施Spd,除进一步促进根系吸收硝态氮且向地上部运输外,根系和叶片NR活性亦有所升高,从较长期的效果看,外源Spd还具有防止铵态氮过度积累、促进幼苗体内氮素代谢趋于正常的作用。

关键词: 天然次生林, 林种, 结构, 层次分析

Abstract: Taking the moderate heat-tolerant cucumber cultivar ‘Jinchun 4’ as test material, a quartz sand culture experiment was conducted in a growth chamber to investigate the effects of exogenous spermidine (Spd) on the nitrogen metabolism of cucumber seedlings under high temperature (42 ℃) stress. Short term high temperature stress, especially within 4 hours, decreased the plant nitrate N content while increased the plant ammonium N content, whereas pretreated with exogenous Spd increased the plant nitrate N and ammonium N contents and nitrate reductase (NR) activity. Longer term high temperature stress increased the root nitrate N content but blocked the nitrate N translocation to shoot, deactivated root NR activity, and increased the ammonium N content in root and leaf significantly. Foliar spraying Spd under high temperature stress promoted the nitrate N translocation from root to shoot, and increased the NR activity in root and leaf. From the viewpoint of long-term effect, exogenous Spd could have the effects of preventing the excessive accumulation of ammonium N in cucumber seedlings, and of promoting the normalization of the  nitrogen metabolism of cucumber seedlings under high temperature stress.

Key words: Natural secondary forest, Forest species, Structure, Analytic hierarchy process