关键词: 抗性基因, 实时荧光定量PCR, Tail-PCR, tetW侧翼序列

Abstract: The author considers that ecology is an objective reality. It is the structure formed by organism and its environment,as well as the function expressed by the structure. Three attributes of ecology, i.e., naturality, sociality and economy are preliminarily commented.

Key words: antibiotic resistance gene, real-time PCR, thermal asymmetric interlaced PCR (Tail-PCR), tetW flanking sequencing