关键词: 蔬菜种植年限, 末端限制性片段多态性分析, 实时荧光定量PCR, 群落结构和丰度

Abstract: Soil testing is the precondition of quantitative fertilization.To test the contents of soil available nutrients needs investments,and there are many errors when converting the tested values into soil nutrient application.Therefore,it is very significant on theory and practice to study principles and models of the semi-quantitative fertilization under the condition of no soil testing.The research results showed that ecological balanced fertilization model can be used as a semi-quantitative fertilization model.It has many strongpoints,such as complete functions,plenty of information,less parameter variations,easy for obtaining parameters,and precise in calculation,etc.

Key words: vegetable cultivation years, terminal restriction fragment length polymorphism (T-RFLP), real-time fluorescent quantitative PCR (real-time PCR), community structure and abundance.