欢迎访问《生态学杂志》官方网站,今天是 分享到:

生态学杂志

• 研究报告 • 上一篇    下一篇

外源一氧化氮对盐胁迫下菊苣生长及渗透调节物质的影响

谷文英1,李兴正1,祈新梅1,高洪文2**   

  1. 1扬州大学动物科学与技术学院, 扬州 225009; 2中国农业科学院北京畜牧兽医研究所, 北京 100193)
  • 出版日期:2013-03-10 发布日期:2013-03-10

Effects of exogenous nitric oxide on the growth and osmotic regulation substances of Cichorium intybus L. under salt stress.

GU Wen-ying1, LI Xing-zheng1, QI Xin-mei1, GAO Hong-wen2**   

  1. (1College of Animal Science and Technology, Yangzhou University, Yangzhou 225009; 2Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193)
  • Online:2013-03-10 Published:2013-03-10

摘要: 利用室内砂培实验,研究了外源性一氧化氮(NO)供体硝普钠(SNP, 0.2 mmol·L-1)对不同浓度NaCl(140和280 mmol·L-1)胁迫下菊苣(Cichorium intybus L. cv. Commander)幼苗生长及渗透调节物质的影响。结果表明:与空白对照相比,随着盐胁迫时间的延长(6~15 d),菊苣叶面积、叶长、叶宽以及叶相对含水量(RWC)明显降低,而脯氨酸含量则显著升高(P<0.05)。HPLC分析表明,根中果糖、葡萄糖和蔗糖含量表现为先升高后下降;1-蔗果三糖和蔗果四糖的含量在低盐胁迫下明显下降,而在高浓度盐胁迫下则有所升高。SNP预处理不仅缓解了盐胁迫对菊苣叶的生长和RWC的抑制,而且使脯氨酸含量和蔗果四糖含量急剧增加(P<0.05),同时降低了果糖、葡萄糖和蔗糖的含量。这说明外源NO能通过增强菊苣幼苗的保水能力,促进渗透调节物质脯氨酸和果聚糖特别是蔗果四糖的生成而增强抵御盐胁迫的能力。

关键词: 转Bt水稻, 褐飞虱, 三唑磷, 溴氰菊酯, 再猖獗

Abstract: A sand culture experiment was conducted in a growth chamber to study the responses of the growth and osmotic regulation substances of Cichorium intybus L. cv. Commander seedlings to the exogenous NO donor, sodium nitroprusside (SNP, 0.2 mmol·L-1), under salt stress (140 and 280 mmol·L-1 of NaCl). As compared with the control, the leaf area, length, width, and relative water content (RWC) of seedlings  under the salt stress had an obvious decrease whereas the leaf proline content increased significantly (P<0.05), and the decrement and increment were timedependent. The highperformance liquid chromatography (HPLC) analysis further indicated that the fructose, glucose and sucrose contents in the seedling’s roots under salt stress decreased after an initial increase, and the root 1-kestose and nystose contents decreased sharply under the stress of 140 mmol·L-1 of NaCl but increased somewhat under the stress of 280 mmol·L-1 of NaCl. Pretreatment with SNP not only alleviated the inhibitory effects of salt stress on the leaf growth and RWC, but also made the leaf proline and nystose contents increased significantly (P<0.05) and the leaf fructose, glucose and sucrose contents decreased. It was suggested that exogenous NO could enhance the resistance of C. intybus seedlings against salt stress by increasing the seedling’s water retention capacity and promoting the synthesis of osmotic regulation substances proline and fructan, especially nystose.

Key words: transgenic Bt rice, Nilaparvata lugens, triazophos, deltamethrin, resurgence.