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铬对中国林蛙蝌蚪生长发育的毒性效应

李勃1,2,王维君1,李忻怡1,张育辉1**   

  1. (1陕西师范大学生命科学学院, 西安 710062; 2陕西省微生物研究所, 西安 710043)
  • 出版日期:2013-03-10 发布日期:2013-03-10

Toxicity effect of chromium on the growth and development of Rana chensinensis tadpoles.

LI Bo1,2, WANG Wei-jun1, LI Xin-yi1, ZHANG Yu-hui1**   

  1. (1College of Life Science, Shaanxi Normal University, Xi’an 710062, China; 2 Shaanxi Microbiology Institute, Xi’an 710043, China)
  • Online:2013-03-10 Published:2013-03-10

摘要: 为评价水域环境中铬元素对两栖动物幼体的急性毒性,将中国林蛙(Rana chensinensis) 28~29期蝌蚪分别暴露于30~35 mg·L-1 Cr(Ⅲ) 6个不同浓度和10~45 mg·L-1 Cr(Ⅵ) 6个不同浓度的水体中,分别在24、48、72和96 h统计蝌蚪的死亡率及半致死浓度(LC50)。结果表明:暴露24、48、72和96 h,Cr(Ⅲ)对蝌蚪的LC50分别为34.09±1.06、33.47±0.65、32.58±0.11和(32.05±0.20) mg·L-1,安全浓度(SC)为(3.21±0.02) mg·L-1;Cr(Ⅵ)对蝌蚪的LC50分别为91.97±5.32、51.19±4.62、35.79±1.40和(28.81±1.87) mg·L-1,安全浓度(SC)为(2.88±0.19) mg·L-1。观察表明:Cr(Ⅲ)的急性毒性是通过与蝌蚪皮肤表面的分泌物结合后粘附在鳃部,导致呼吸障碍致死;而Cr(Ⅵ)的强氧化性可导致蝌蚪的表皮溃变,鳃部萎缩致死;另外,将28~29期蝌蚪暴露于安全浓度(SC)以下的含铬水体进行慢性实验,通过检测蝌蚪的体长、体重和完全变态时间显示,低浓度的Cr(Ⅲ)和Cr(Ⅵ)对蝌蚪的生长发育仍具有一定的抑制作用,并可导致畸型发生,其作用强度呈现剂量效应,但时间累积效应不规律。

关键词: 刺槐人工林, 落叶灌木, 退耕草地, 沙蒿群落, 细根生物量, 生产量

Abstract: In order to evaluate the acute toxicity of chromium (Cr) on amphibian larvae, the Rana chensiensis tadpoles at the stages 28-29 were exposed to the water contaminated by 30-35 mg·L-1 of Cr(III) and 10-45 mg·L-1 of Cr(VI), with the death rate of the tadpoles and the sublethal concentration (LC50) of Cr(III) and Cr(VI) for 24, 48, 72, and 96 h determined, respectively. Both the Cr(III) and the Cr(VI) had obviously acute toxicity on the tadpoles. The LC50 of Cr(III) for 24, 48, 72, and 96 h was 34.09±1.06, 33.47±0.65, 32.58±0.11, and 32.05±0.20 mg·L-1, and the LC50 of Cr(VI) was 91.97±5.32, 51.19±4.62, 35.79±1.40, and 28.81±1.87 mg·L-1, respectively. The safe concentration (SC) of Cr(III) and Cr(VI) was 3.21±0.02 and 2.88±0.19 mg·L-1, respectively. The acute toxicity of Cr(III) was from its combination with the skin secretion of the tadpoles, forming a complex compound sticking on the gill chamber, and then, causing the tadpoles dead from breath obstacle; whereas the acute toxicity of the Cr(VI) was due to its strong oxidizing property, causing the tadpoles skin fester, gill atrophy, and ultimately, the mortality of the tadpoles. The assessment of the sublethal concentrations of Cr(III) and Cr(VI) on the growth and development of R. chensiensis tadpoles showed that Cr was a teratogenic substance which could restrain the growth and development of the tadpoles. This effect also had a doseeffect relationship with the Cr concentrations, but the time cumulative effect was irregular.

Key words: Robinia pseudoacacia plantation, deciduous shrubs, abandoned grassland, Artemisia desertorum community, fine root biomass,   production.