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恒温与变温对不同生活史阶段斑马鱼热耐受性的影响

王国强1,夏继刚2*   

  1. (1西安文理学院师范学院, 西安 710065; 2进化生理与行为学实验室, 重庆市动物生物学重点实验室, 重庆师范大学生命科学学院, 重庆 401331)
  • 出版日期:2019-07-10 发布日期:2019-07-10

Effects of constant and diel-fluctuating temperature on thermal tolerance of zebrafish at different life-history stages.

WANG Guo-qiang1, XIA Ji-gang2*   

  1. (1Teachers’ College, Xi’an University, Xi’an 710065, China; 2Laboratory of Evolutionary Physiology and Behavior, Chongqing Key Laboratory of Animal Biology, College of Life Sciences, Chongqing Normal University, Chongqing 401331, China).
  • Online:2019-07-10 Published:2019-07-10

摘要: 为了探究恒温与变温以及生活史阶段对斑马鱼(Danio rerio)热耐受性的影响,将新孵出的斑马鱼仔鱼、幼鱼以及性成熟雌鱼与雄鱼分别于恒温(28 ℃)和变温(26~30 ℃)条件下驯养2周,之后,采用临界温度法测定不同温度驯化下仔鱼、幼鱼以及雌鱼和雄鱼的临界高温CTmax、致死高温LTmax、临界低温CTmin、致死低温LTmin等热耐受性参数。结果表明:驯化温度、生活史阶段以及二者交互作用对CTmaxLTmaxCTminLTmin均影响显著(P<0.05),驯化温度和性别对斑马鱼成鱼的热忍耐参数无显著性影响(P> 0.05);仔鱼的热耐受性较差,低于幼鱼和成鱼,表现为CTmaxLTmax低而CTminLTmin高,这可能主要与不同生活史阶段实验鱼的生活习性及其栖息环境有关;变温对幼鱼和成鱼热耐受性无显著影响,但导致仔鱼体长增加、热忍耐范围变窄,提示仔鱼生长和热耐受性可能存在权衡。

关键词: 人-水耦合系统, 社会水文学, 水资源的可持续利用, 双向互馈

Abstract: To examine the effects of constant and diel-fluctuating temperature on thermal tolerance of zebrafish (Danio rerio) at different lifehistory stages, the newly hatched fry, juvenile fish, and sexually mature females and males were acclimated to constant temperature (28 ℃) and diel-fluctuating temperature (26-30 ℃) for two weeks. Then, the critical thermal maxima (CTmax), lethal thermal maxima (LTmax), critical thermal minima (CTmin), and lethal thermal minima (LTmin) of zebrafish at different life-history stages were measured using the critical thermal methodology of Becker and Genoway. Acclimation temperature, life-history stage, and their interactions had significant effects on thermal tolerance of zebrafish (P<0.05), whereas acclimation temperature and gender had no significant effect on thermal tolerance of adult zebrafish (P>0.05). The thermal tolerance of larvae was weaker than that of juvenile and adult fish, with lowerCTmax and LTmax and higher CTmin and LTmin. These characteristics might be related to the living habits and habitat environment of fishes at different life-history stages. The diel-fluctuating temperature had no effect on the thermal tolerance of juvenile and adult fish, but it increased body length and decreased thermal endurance scope in larvae, suggesting a tradeoff between growth and thermal tolerance.

Key words: socio-hydrology, coupled human-water system, sustainable utilization of water resources, two-way feedbacks.