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南亚热带典型乡土阔叶人工林土壤微生物碳源利用及功能多样性

陈超凡1,吴水荣2,覃林1*,范垚城1,谭玲1,郭文福3#br#   

  1. (1广西大学林学院, 南宁 530004; 2中国林业科学研究院林业科技信息研究所, 北京 100091; 3中国林业科学研究院热带林业实验中心, 广西凭祥 532600)
  • 出版日期:2016-05-10 发布日期:2016-05-10

Soil microbial carbon source utilization and functional diversity of typical native broadleaved plantations in south subtropical China.

CHEN Chao-fan1, WU Shui-rong2, QIN Lin1*, FAN Yao-cheng1, TAN Ling1, GUO Wen-fu3#br#   

  1. (1College of Forestry, Guangxi University, Nanning 530004, China; 2Research Institute of Forestry Policy and Information, Chinese Academy of Forestry, Beijing 100091, China; 3Experimental Center of Tropical Forestry, Chinese Academy of Forestry, Pingxiang 532600, Guangxi, China).
  • Online:2016-05-10 Published:2016-05-10

摘要: 研究南亚热带典型乡土阔叶人工林土壤微生物碳源利用及功能多样性对红壤丘陵地区植被恢复重建树种的选择具有重要意义。本研究采用Biolog-Eco技术,对4种典型乡土阔叶人工林(红椎林、米老排林、火力楠林和山白兰林)和外来速生桉树人工林不同土壤深度(0~20、20~40和40~60 cm)土壤微生物的碳源利用及功能多样性进行分析。结果表明,5种林分土壤微生物对单一碳源利用的最佳培养时间为108 h。各林分土壤微生物对碳源的利用能力受时间因素的影响显著,并随土层深度的增加而下降;在培养108 h时,4种乡土阔叶人工林土壤微生物对碳源利用能力的差异不明显,但均高于桉树林。Shannon指数和Simpson指数表明,4种乡土阔叶人工林各土层的土壤微生物功能多样性均大于桉树林,但乡土阔叶人工林土壤微生物功能多样性在各土层的差别各异。对31种碳源吸光值进行主成分分析和聚类分析表明,糖类、氨基酸类、多聚物类、羧酸类和芳香类碳源是不同林分之间土壤微生物功能多样性差异的敏感碳源。

关键词: 米槠次生林, 皆伐, 采伐剩余物, 分解速率, 化学组成

Abstract: Understanding soil microbial carbon source utilization and functional diversity of typical native broadleaved artificial forests is of great importance in tree species selection for vegetation restoration and reconstruction of hilly red soil areas in south subtropical China. By using BiologEco technology, we studied the soil microbial carbon source utilization and functional diversity at different soil depths (0-20, 20-40 and 40-60 cm) of four typical native broadleaved planted forests (Castanopsis hystrix, Mytilaria laosensis, Michelia macclurei and Paramichelia bailonii) and exotic fastgrowing eucalyptus plantation in the Fubo Forest Farm in Pingxiang, Guangxi. The results showed that the optimum incubation time for the capacity of soil microbes using monocarbon source in the five planted forests was 108 h. The capacity of soil microbial carbon source utilization of the different planted forests increased with the incubation time, and decreased with the soil depth. At the incubation time of 108 h, the capacity of soil microbial carbon source utilization among the four native broadleaved planted forests had no significant difference, but it was higher than that of the eucalyptus plantation. The Shannon index and Simpson index indicated that the functional diversity of soil microbial community of the four native broadleaved planted forests were different at various soil depths, and they were higher than those of the eucalyptus plantation at the same soil horizon. The principal component analysis and cluster analysis of 31 carbon sources indicated that carbohydrate, amino acid, polymerizer, carboxylic acid and aromatic carbon were sensitive carbon sources to the functional diversity of soil microbial communities in the different planted forests.

Key words: Castanopsis carlesii secondary forest, clear-cutting, logging residue, decomposition rate, chemical composition.