欢迎访问《生态学杂志》官方网站,今天是 分享到:

生态学杂志

• 研究报告 • 上一篇    下一篇

矢车菊素-3-葡萄糖苷对重金属镉胁迫后大鼠氧化损伤的缓解作用

李晔1,蔺怀1,2,张倩茹2*,白卫滨3#br#   

  1. 1沈阳大学环境学院, 沈阳 110044; 2中国科学院沈阳应用生态研究所, 沈阳 110016; 3暨南大学食品科学与工程系, 广州 510632)
  • 出版日期:2016-05-10 发布日期:2016-05-10

Ameliorative effect of cyanidin-3-glucoside on heavy metalinduced oxidative stress in the liver of SD rats.

LI Ye1, LIN Huai1,2, ZHANG Qian-ru2*, BAI Wei-bin3#br#   

  1. (1Environment College, Shenyang University, Shenyang 110044, China; 2Institute of Applied Ecology, Academy of Sciences, Shenyang 110016, China; 3Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China).
  • Online:2016-05-10 Published:2016-05-10

摘要: 为研究矢车菊素-3-葡萄糖苷(Cy-3-glu)对重金属镉胁迫后的脂质过氧化和氧化应激反应的缓解作用,将雄性SD大鼠随机分成4组(每组5只)分别灌服Cy-3-glu(100、200 mg·kg-1),同时腹腔注射重金属镉(Cd),收集组织样本进行测定。结果显示,灌服Cy-3-glu的SD大鼠能明显缓解由重金属镉胁迫导致的大鼠体重下降,并且肝脏、肾脏、睾丸等脏器指数随Cy-3-glu剂量的升高而呈显著或极显著上升;Cy-3-glu能显著改善由重金属镉引起的肝脏ROS、MDA含量升高;抗氧化酶系活性也随之发生改变,Cy-3-glu(200 mg·kg-1)保护组能够显著提高大鼠SOD酶活,但对于POD、CAT酶活性并未表现一定的量效关系。表明Cy-3-glu可以使肝脏的抗氧化潜力明显恢复,通过清除自由基、缓解脂质过氧化、激活相关抗氧化酶活性,从而降低机体的ROS、MDA含量,缓解由重金属镉引起的大鼠肝脏氧化应激反应,发挥保护作用。

关键词: 可溶性有机碳, 土柱, 淋溶, 吸附

Abstract: The present study examined the possible protective effect of Cyanidin-3-glucoside (Cy-3-glu) against Cdinduced damage on lipids peroxidation and stress biochemical parameters of rats. SpragueDawley rats were randomly divided into 4 groups of 5 animals each and treated orally with Cd (5 mg·kg-1),  Cy-3-glu (100 and 200 mg·kg-1) alone or in combination for 5 days. The result showed that the treatment with Cy-3-glu obviously alleviated the body weight loss of rat caused by heavy Cd stress, and the liver, kidney and testis viscera index were significantly or extremely significantly increased with Cy-3-glu dose. Cd exposure induced significant increase in the activities of reactive oxygen species (ROS), lipid per-oxidation levels (MDA) in the exposed groups compared to the control group, and the antioxidant enzymes activities also changed, but cotreatment with Cy-3-glu (200 mg·kg-1) significantly improved the damage induced by Cd and the SOD enzyme activities were significantly increased. The POD and CAT enzyme activities did not show concentrationresponse relationships. The antioxidant potential in liver was markedly restored with a significant decline in ROS and MDA levels. In conclusion, these results suggest that Cy-3-glu could protect the rat liver against Cd-induced injury by increasing the activities of antioxidant enzymes and reducing oxidative stress.

Key words: dissolved organic carbon, soil core, leach, adsorption.