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• 研究报告 • 上一篇    下一篇

3株尖孢镰刀菌遗传多样性的ISSR分析

张述义1,李新凤2,韦晓艳2,王建明2**   

  1. 1山西省农业科学院旱地农业研究中心, 太原 030006; 2山西农业大学农学院, 山西太谷 030801)
  • 出版日期:2013-05-10 发布日期:2013-05-10

Genetic diversity of 33 Fusarium oxysporum strains: An ISSR analysis.

ZHANG Shu-yi1, LI Xin-feng2,WEI Xiao-yan2,WANG Jian-ming2**   

  1. (1Center of Dryland Agricultural Research, Shanxi Academy of Agricultural Science, Taiyuan 030006, China; 2Shanxi Agricultural University, Taigu 030801, Shanxi, China)
  • Online:2013-05-10 Published:2013-05-10

摘要: 为明确尖孢镰刀菌种内各菌株间的遗传差异与亲缘关系,采用ISSR分子标记技术对33株地理来源不同的尖孢镰刀菌进行了遗传多样性分析。结果表明:利用筛选出的11条引物扩增出105条条带,其中多态性条带91条,多态性位点比例为86.7%;遗传相似性与聚类分析结果〖JP2〗供试菌株间的遗传相似系数为0.606~0.962,平均0.756,当遗传相似系数为0.962时,供试的33株菌可被全部区分开。表明,尖孢镰刀菌基因组在SSR区域具有丰富的多态性。寄主来源相同的供试菌株间的遗传相似性与其地理来源有一定的相关性。

关键词: 耕作方式, 复播大豆, 光合特性, 经济效益

Abstract:

To clarify the genetic difference and phylogenic relationship among the strains of Fusarium oxysporum, the genetic diversity of 33 F. oxysporum strains from different geographic origin was examined by inter-simple sequence repeats (ISSR). A total of 105 bands were amplified with 11 selected ISSR primers, 91 (86.7%) of which were polymorphic. The cluster analysis based on the ISSR data showed that the genetic similarity among the strains ranged from 0.606 to 0.962, with an average of 0.756, and all the strains could be distinguished at 0.962, which indicated that the SSR loci in F. oxysporum genomes were rich in polymorphism. There existed definite correlations between the genetic similarity and geographic origin of the strains isolated from the same hosts.
 

Key words: tillage method, quadric sowing soybean, photosynthetic characteristics, economic benefit.