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不同砧穗组合葡萄植株对部分根区干旱的生理生化响应

綦伟1,3;厉恩茂1,2;翟衡1,2;王晓芳1;杜远鹏1   

  1. 1山东农业大学园艺科学与工程学院, 山东泰安 271018; 2山东农业大学作物生物学国家重点实验室, 山东泰安 271018;3山东省蓬莱市葡萄与葡萄酒局, 山东蓬莱 265600
  • 收稿日期:2006-12-12 修回日期:1900-01-01 出版日期:2008-02-21 发布日期:2008-02-21

Physiological and biochemical responses of different scion/rootstock combinations grapevine to partial rootzone drought.

QI Wei1,3; LI En-mao1,2; ZHAI Heng1,2; WANG Xiao-fang1; DU Yuan-peng1    

  1. 1College of Horticultural Science and Engineering, Shandong Agricultural University, Tai’an 271018, Shandong, China; 2State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, Shandong, China; 3Penglai Bureau of Vine and Wine, Penglai 265600, Shandong, China

  • Received:2006-12-12 Revised:1900-01-01 Online:2008-02-21 Published:2008-02-21

摘要: 利用自制木箱对嫁接在3309C、420A和110R砧木上的玛瓦斯亚葡萄(M)进行双侧根区交替灌溉(AI)和单侧灌溉(UI)两种水分胁迫处理,以探讨不同葡萄砧穗组合对干旱的适应能力.结果表明:水分胁迫导致葡萄叶片ABA浓度大幅度增加,AI和UI的3种组合平均分别提高了267.5%和394.7%,而气孔导度和蒸腾速率显著下降.UI处理的3种组合叶片SOD和CAT活性显著增加,脯氨酸(Pro)含量极显著增加,且均以M/110R增幅较大,M/3309C增幅较小;而AI处理的SOD和CAT活性增加较小,但Pro含量显著增加.干旱胁迫导致葡萄叶片质膜透性、MDA和H2O2含量极显著增加,以M/3309C增加最多,M/110R增加较少.不同砧穗组合适应干旱逆境的能力主要取决于砧木品种,110R适应干旱逆境的能力强于420A和3309C,其对应的嫁接植株的生长量亦呈同样趋势.与根区单侧灌溉相比,双侧根区交替灌溉对植株造成的伤害较小,是可推广的节水灌溉方式.

关键词: 生态特征, 环境因子, 恢复模式, 苔草草丘, 太阳岛湿地

Abstract: By using self-made wooden boxes with two separated zones, the grapevine Vitis vinifera cv. Malvasia (M) grafted on rootstocks 3309C, 420A and 110R, respectively, was planted, and the physiological and biochemical responses of these scion/rootstock combinations to bilateral alternative irrigation (AI) and unilateral irrigation (UI) were studied. The results showed that in treatments AI and UI, the average leaf ABA content of test scion/rootstock combinations increased by 267.5% and 394.7%, respectively, while stomatal conduction and transpiration decreased markedly. In treatment UI, the leaf SOD and CAT activities and Pro content were notably enhanced, with the greatest increment in M/110R and followed by in M/420A and M/3309C; while in treatment AI, the leaf SOD and CAT activities of test scion/rootstock combinations enhanced slightly and Pro content increased markedly. UI induced a remarkable increase of leaf relative electronic conductivity and MDA and H2O2 contents, with the highest increment in M/3309C and the lowest in M/110R. In summary, the drought resistance of different scion/rootstock combinations grapevine mainly depended on the variety of rootstock. 110R had a higher drought-resistance than 420A and 3309C. Comparing with unilateral irrigation, bilateral alternative irrigation had lesser damage to grapevine, being a profitable water-saving irrigation technique.

Key words: ecological characteristics, environmental factor, restoration method, Carex tussock, Sun Island wetland