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水分含量对紫色土自养微生物丰度与活性的影响

魏万玲,程永毅,周志峰,王明霞*   

  1. (西南大学资源环境学院, 重庆 400715)
  • 出版日期:2020-02-10 发布日期:2020-02-10

Effects of water content on the abundance and activity of autotrophic microbes in purple soil.

WEI Wan-ling, CHENG Yong-yi, ZHOU Zhi-feng, WANG Ming-xia*   

  1. (College of Resources and Environment, Southwest University, Chongqing 400715, China).
  • Online:2020-02-10 Published:2020-02-10

摘要: 通过室内培养试验,研究土壤水分对紫色土自养微生物丰度与活性的影响。土壤含水量(质量百分比)设置为4个处理,分别为10%(W10)、15%(W15)、25%(W25)及淹水(WL),于室温下培养28天。分别于培养的第3、7、14、28天取样,测定土壤有机碳含量、cbbL基因(1,5-二磷酸核酮糖羧化酶大亚基基因)丰度、RubisCO(1,5-二磷酸核酮糖羧化酶)酶活性,并分析它们之间的相关性。结果表明:土壤cbbL基因丰度于培养的前14天呈现随含水量增加而升高的趋势,且淹水处理(WL)在整个培养期间其cbbL丰度均处于最高水平。各处理土壤的RubisCO酶活性在培养初期(第3天)无显著差异,培养后期(第28天)低含水量(W10和W15)处理中RubisCO酶活性显著低于高含水量的W25及WL处理(P<0.05)。单因素重复检测方差分析结果表明,含水量显著影响土壤有机碳含量(F=11.91,P=0.03)、cbbL丰度(F=42.33,P<0.01)和RubisCO酶活性(F=51.55,P<0.01)。相关性分析表明,土壤cbbL丰度与RubisCO酶活性呈显著正相关(R2=0.23,P<0.01),而它们均与土壤有机碳含量呈显著负相关。综上,土壤水分含量是影响紫色土自养微生物丰度和活性的关键因子,它们均随含水量升高而增加,此结果可为揭示土壤碳转化对土壤湿度变化的响应机制提供重要的基础信息。

关键词: 去除率, 总氮, 化学需氧量, pH, 总磷, 湿地植物组合

Abstract: With cbbL (ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) large-subunit gene) and RubisCO (1,5-bisphosphate ribulose carboxylase) enzyme as markers, we investigated the effects of soil water content on the abundance and activity of soil autotrophic microbes in an indoor incubation experiment for 28 days. There were four treatments with different soil water contents (mass percentage of water to dry soil), including 10% (W10), 15% (W15), 25% (W25), and waterlogging (WL). Soil samples were harvested after 3, 7, 14, and 28 days of incubation, with soil organic carbon content, cbbL abundance, and RubisCO enzyme activity being measured. The results showed that soil cbbL abundance increased with increasing soil water content during the first 14 days. WL treatment had the highest cbbL abundance during the whole period of incubation. At the initial stage of incubation (day 3), there was no significant difference of soil RubisCO enzyme activity among these treatments (P<0.05). At the end of incubation (day 28), however, RubisCO enzyme activity in the treatments with lower water content (W10 and W15) was significantly lower than that in the treatments with higher water content (W25 and WL; P<0.05). Soil organic carbon content (F=11.91,P=0.03), cbbL abundance (F=42.33,P<0.01), and RubisCO enzyme activity (F=51.55,P<0.01) significantly varied among different treatments. Soil cbbL abundance and RubisCO enzyme activity were positively correlated (R2=0.23,P<0.01), and both of them were negatively correlated with soil organic carbon content. Our results indicated that soil water content played an important role in controlling the abundance and activity of soil autotrophic microbes, which should be valuable for further mechanistic understanding of responses of soil carbon transformation to soil moisture variation.

Key words: total phosphorus, wetland plant combination, pH., removal efficiency, total nitrogen, chemical oxygen demand