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海洋微生物ZD02信号降解酶基因aiiA克隆及其生物信息学分析

丁贤1**,孙威文2,张殿昌1,翁雄1,林黑着1,周世宁2   

  1. (1中国水产科学研究院南海水产研究所农业部南海渔业资源开发利用重点实验室, 广州 510300;  2 中山大学生命科学学院有害生物防治与资源利用国家重点实验室, 广州 510275)
  • 出版日期:2013-08-10 发布日期:2013-08-10

Cloning and bioinformatic analysis of signal-degrading enzyme gene aiiA from marine bacterium ZD02.

DING Xian1**, SUN Wei-wen2, ZHANG Dian-chang1, WENG Xiong1, LIN Hei-zhe1, ZHOU Shi-ning2   

  1. (1Key Laboratory of South China Sea Fisheries Resources Exploitation & Utilization, Ministry of Agriculture, China; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; 2 State Key Laboratory for Biocontrol, School of Life Sciences, Zhongshan (Sun Yatsen) University, Guangzhou 510275, China)
  • Online:2013-08-10 Published:2013-08-10

摘要: 根据细菌群体感应信号降解酶(AiiA)基因设计简并引物,从海洋微生物ZD02基因组中扩增编码AiiA的基因aiiA,筛选其阳性克隆,测序后分析其基因序列。结果表明:筛选到的阳性克隆ZD02aiiA序列全长753 bp(Genbank登录号:KC756214),存在一个开放阅读框(ORF),编码由250个氨基酸残基组成的多肽,预测分子量为28.1 kDa,蛋白等电点为4.78。由该序列推导得到的氨基酸残基序列含有一个保守结构域(Lactamase-B) (34AA~235AA),并预测了AiiA的三级结构。以上研究为该序列的重组表达及其相关活性研究奠定了基础。

关键词: 镉, 褪黑素,  , 化学形态, 水稻

Abstract: By using a pair of degenerate primers designed according to the known conserved sequence of aiiA, the gene aiiA encoding the quorum sensing signal degrading enzyme (AiiA) in the genome of marine bacterium ZD02 was amplified by PCR, and the PCR products (ZD02 aiiA) were cloned, sequenced, and analyzed. The ZD02 aiiA was 753 bp (Genbank accession number: KC756214), containing an open reading frame (ORF) which encoded a polypeptide chain AiiA of 250 amino acids residues with a molecular weight of 28.1 kDa and an isoelectric point value of 4.78. A conservative domain (Lactamase-B) (34AA-235AA) in the sequence was identified, and the 3D structure of deduced AiiA protein was further predicted. This study would provide a foundation for the expression of the sequence and its bio-activity analysis.

Key words:  , rice, melatonin, Cd, chemical form.