欢迎访问《生态学杂志》官方网站,今天是 分享到:

生态学杂志

• 研究报告 • 上一篇    下一篇

催乳素受体与黑线仓鼠胎产仔数的关联性

薛慧良,王东宽,徐金会,徐来祥**   

  1. (曲阜师范大学生命科学学院, 曲阜 273165)
  • 出版日期:2013-11-10 发布日期:2013-11-10

Correlation of prolactin receptor with litter size of Striped Hamster (Cricetulus barabensis).

XUE Hui-liang, WANG Dong-kuan, XU Jin-hui, XU Lai-xiang**   

  1. (College of Life Sciences, Qufu Normal University, Qufu 273165, Shandong, China)
  • Online:2013-11-10 Published:2013-11-10

摘要:

黑线仓鼠是生态系统的主要功能群之一,胎产仔数是影响黑线仓鼠种群数量的一个主要因素,催乳素受体(PRLR)又是控制动物胎产仔数的一个主效基因,但PRLR与黑线仓鼠胎产仔数的关联性还不清楚。本研究以具有胎产仔数记录的90只黑线仓鼠为材料,检测PRLR 5′端的SNP,并分析单倍型结构,同时检测试验个体发情期卵巢PRLR的表达量。分析了PRLR 5′端的单倍型结构和发情期卵巢PRLR的表达量与胎产仔数的关联性。结果表明,在黑线仓鼠PRLR 5′端共检测到3个多态性位点,5种单倍型结构;在不同的单倍型结构间,胎产仔数均存在显著差异;除H1和H2之间、H4和H5之间外,其余单倍型间,黑线仓鼠发情期卵巢PRLR的表达量均存在显著差异;并且发情期卵巢PRLR的表达量与胎产仔数存在显著正相关(r=0.982)。因此,在PRLR 5′端检测到的多态性位点显著影响黑线仓鼠的胎产仔数,这为有效控制黑线仓鼠种群数量从分子水平上提供了理论依据。

 

关键词: 刺槐, 磷, 碳, 氮, 林龄, 黄土高原

Abstract: Striped Hamster (Cricetulus barabensis) is one of the main functional groups in the regulation of cropland ecosystems. Litter size is one of the main factors affecting the population fluctuation of Striped Hamster, and prolactin receptor (PRLR) is the main effective gene controlling the litter size of animals. However, how the PRLR works on the population fluctuation of Striped Hamster is still unclear. In this study, ninety Striped Hamster individuals with the records of litter size were taken as the test materials to detect the SNP of the 5′ end of PRLR, analyze the haplotype structure, and examine the expression quantity of PRLR in ovaries during the oestrum, aimed to understand the correlations of the haplotype structure of the 5′ end of PRLR and the expression quantity of PRLR in ovaries during the oestrum with the litter size. A total of 3 polymorphic loci and 5 haplotypes (H1, H2, H3, H4, and H5) were detected in the 5′ end of the PRLR. Among the 5 halotypes structure, there existed significant difference in the litter size. Except between H1 and H2 and between H4 and H5, the expression quantity in the PRLR in ovaries during the oestrum had significant difference among the other haplotypes, and this expression quantity was significantly positively correlated with litter size (r= 0.982). Therefore, the polymorphic loci detected in the 5′ end of PRLR significantly affected the litter size, which could provide theoretical basis at molecular level for the effective control of Striped Hamster population.

Key words: carbon, Loess Plateau., nitrogen, plantation age, Robinia pseudoacacia, phosphorus