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O3和干旱胁迫对元宝枫叶片气孔特征的复合影响

文志1,2,王丽1**,王效科2,李丽2,崔健2   

  1. 1中南大学生命科学学院, 长沙 410013; 2中国科学院生态环境研究中心城市与区域生态国家重点实验室, 北京 100085)
  • 出版日期:2014-03-10 发布日期:2014-03-10

Combined effects of ozone and drought on leaf stomata of Acer truncatum.

WEN Zhi1,2, WANG Li1**, WANG Xiao-ke2, LI Li2, CUI Jian2   

  1. (1School of Life Sciences, Central South University, Changsha 410013, China; 2State Key Laboratory of Urban and Regional Ecology, Research Center for EcoEnvironmental Sciences, Chinese Academy of Sciences, Beijing 100085, China)
  • Online:2014-03-10 Published:2014-03-10

摘要: 利用开顶式气室(OTC)研究了O3胁迫(空气O3浓度加118 μg·m-3)、干旱胁迫(土壤水分保持在田间持水量的40%~50%)以及O3与干旱复合胁迫(空气O3浓度加118 μg·m-3和土壤水分保持在田间持水量的40%~50%)对元宝枫(Acer truncatum Bunge.)叶片气孔特征的影响。结果表明:O3胁迫41 d时气孔密度变化不明显,62和110 d时显著增加(P<0.05);干旱胁迫和复合胁迫下气孔密度增加显著(P<0.05);O3、干旱及复合胁迫处理110 d气孔面积、周长、长度和宽度明显减小(P<0.05),气孔指数明显增加(P<0.05);各胁迫气孔开度均明显减小(P<0.05),O3和干旱对气孔开度影响存在协同作用,干旱减小了O3胁迫下的气孔开度。O3和干旱胁迫下气孔特征响应不同表明影响气孔的机制不同,O3对气孔影响具有累积效应,干旱可限制O3摄入量从而减小了O3对植物的伤害。

关键词: 种植时间, 粗放经营, 毛竹,  , 多样性, 速效钾, 有效磷, 土壤细菌和真菌群落, DGGE

Abstract: An open-top chambers (OTCs) experiment was carried on the combined effects of ozone and drought on leaf stomata of Acer truncatum Bunge. Four treatments were designed: control (ambient air without drought stress), drought (ambient air with 40-50% of soil waterholding capacity), enhanced ozone (ambient air with additional 118 μg·m-3 ozone without drought stress), and enhanced ozone and drought (ambient air with additional 118 μg·m-3 ozone and 40-50% of soil waterholding capacity). 41 days after treatment, the stomatal density did not change significantly under ozone exposure, but increased significantly under drought stress and combined stress (P<0.05). 41 and 62 days after treatment with ozone and drought stresses, there were no significant differences in stomatal area, length and width among treatments (P>0.05), while 110 days after treatment with ozone and drought, the stomatal area, length and width were smaller than those of the control (P<0.05). Compared with the control, stomatal index increased significantly 110 days after treatment by ozone and drought (P<0.05). The stomatal opening degree reduced significantly when A. truncatum was exposed to enhanced ozone and drought (P<0.05). These results suggested that accumulating effects were reflected on stomatal characteristics of A. truncatum under ozone stresses. Ozone and drought stresses influenced the stomatal characteristics interactively. The synergistic effect of ozone and drought stresses was evidenced on the stomatal aperture. It was suggested that drought could protect plants against ozone-induced damage by causing the closure of stomata and preventing the entry of ozone into the leaves.

Key words: Phyllostachys edulis, extensive management, cultivation history, diversity, available K, available P., soil bacterial and fungal communities, DGGE